DMOG
Based on 86 publication(s) in Google Scholar
DMOG (Dimethyloxallyl Glycine) is a cell permeable and competitive inhibitor of HIF-PH, which results in HIF-1α stabilisation and accmulation in vitro and in vivo. DMOG is an α-ketoglutarate analogue and inhibits α-KG-dependent hydroxylases. DMOG acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal. DMOG induces cell autophagy.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 89464-63-1
- Formula: C6H9NO5
- Molecular Weight:175.14
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DMOG
More- Cell Res. 2021 Sep;31(9):980-997. [Abstract]
- Bioact Mater. 2020 Oct 26;6(4):1175-1188. [Abstract]
- Autophagy. 2026 May;22(5):1021-1043. [Abstract]
- Sci Immunol. 2026 Apr 3;11(118):eaeb6484. [Abstract]
- Redox Biol. 2026 Feb:89:104008. [Abstract]
- Mol Cell. 2026 Jun 25:S1097-2765(26)00378-3. [Abstract]
- Theranostics. 2020 Jun 12;10(16):7409-7421. [Abstract]
- Acta Pharm Sin B. 2025 Sep;15(9):4673-4691. [Abstract]
- Adv Sci (Weinh). 2025 Aug;12(29):e17128. [Abstract]
- Adv Sci (Weinh). 2025 Jul;12(25):e2414504. [Abstract]
- Cell Rep Med. 2026 Apr 29:102784. [Abstract]
- Cell Death Differ. 2026 Jun 24. [Abstract]
- Biomaterials. 2022 Jun;285:121530. [Abstract]
- Cell Death Differ. 2021 May;28(5):1593-1609. [Abstract]
- Cell Death Dis. 2026 Mar 30;17(1):430. [Abstract]
- J Transl Med. 2023 Mar 27;21(1):224. [Abstract]
- Chin Med J (Engl). 2025 Sep 17. [Abstract]
- Oncogene. 2023 Jan;42(3):184-197. [Abstract]
- BMC Med. 2024 Feb 5;22(1):57. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2025;19(8):101517. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2022;13(4):1057-1072. [Abstract]
- Am J Chin Med. 2025;53(8):2561-2578. [Abstract]
- ACS Appl Mater Interfaces. 2023 Jan 11;15(1):364-377. [Abstract]
- Cell Rep. 2026 Jan 28;45(2):116922. [Abstract]
- Cell Rep. 2024 Nov 10;43(11):114915. [Abstract]
- Cell Rep. 2022 Apr 12;39(2):110656. [Abstract]
- Br J Pharmacol. 2022 Apr;179(7):1304-1318. [Abstract]
- Front Immunol. 2018 Jul 23:9:1667. [Abstract]
- Redox Rep. 2026 Dec 31;31(1):2667673. [Abstract]
- Antioxid Redox Signal. 2025 Aug;43(4-6):189-214. [Abstract]
- J Ethnopharmacol. 2024 Aug 10:330:118235. [Abstract]
- Antioxid Redox Signal. 2022 Jul;37(1-3):40-53. [Abstract]
- Ecotoxicol Environ Saf. 2024 Jul 1:279:116481. [Abstract]
- Cell Mol Life Sci. 2024 Dec 27;82(1):21. [Abstract]
- Cell Mol Life Sci. 2024 Jun 17;81(1):270. [Abstract]
- Cell Mol Life Sci. 2022 Nov 30;79(12):611. [Abstract]
- Biochem Pharmacol. 2022 May;199:115017. [Abstract]
- Cancer Gene Ther. 2023 May;30(5):647-658. [Abstract]
- Mol Metab. 2023 Jul:73:101747. [Abstract]
- Drug Des Devel Ther. 2025 Aug 2:19:6647-6664. [Abstract]
- Eur J Pharmacol. 2026 Jan 12:1011:178480. [Abstract]
- Cancer Biol Ther. 2016 Jun 2;17(6):625-34. [Abstract]
- Biomolecules. 2025 Feb 10;15(2):256. [Abstract]
- Mucosal Immunol. 2022 Feb;15(2):268-278. [Abstract]
- Arthritis Res Ther. 2025 Sep 26;27(1):180. [Abstract]
- Transl Oncol. 2026 Jun 18:71:102847. [Abstract]
- Cell Signal. 2021 Nov:87:110140. [Abstract]
- Front Physiol. 2020 Jun 30:11:669. [Abstract]
- Clin Immunol. 2024 Aug:265:110285. [Abstract]
- Curr Issues Mol Biol. 2024 Mar 13;46(3):2386-2397. [Abstract]
- Epigenomes. 2022 Sep 30;6(4):32. [Abstract]
- J Biol Chem. 2015 Jan 16;290(3):1389-94. [Abstract]
- J Tradit Complement Med. 2025 Jun 14.
- Vet Res. 2025 Apr 30;56(1):94. [Abstract]
- J Neurotrauma. 2019 Dec 15;36(24):3394-3409. [Abstract]
- Arch Med Res. 2025 Aug 22;56(8):103295. [Abstract]
- J Dent Sci. 2021 Jul;16(3):937-947. [Abstract]
- Eur Cell Mater. 2021 Feb 3:41:142-152. [Abstract]
- J Reprod Immunol. 2026 Feb 11:174:104848. [Abstract]
- J Cancer. 2022 Mar 28;13(7):2061-2073. [Abstract]
- Neuroscience. 2019 Sep 1:415:31-43. [Abstract]
- Tissue Cell. 2026 Jun 17:103:103703. [Abstract]
- Am J Cancer Res. 2024 Feb 15;14(2):727-743. [Abstract]
- BMC Mol Cell Biol. 2024 Apr 23;25(1):13. [Abstract]
- Metallomics. 2024 Oct 4;16(10):mfae044. [Abstract]
- Acta Histochem. 2019 Aug;121(6):695-703. [Abstract]
- Reprod Sci. 2023 Mar;30(3):928-951. [Abstract]
- PLoS One. 2022 May 16;17(5):e0268445. [Abstract]
- Gene. 2024 Mar 10:898:148077. [Abstract]
- Thorac Cancer. 2019 Apr;10(4):676-685. [Abstract]
- Biochem Biophys Res Commun. 2019 Feb 12;509(3):797-802. [Abstract]
- ChemistrySelect. 2025 Mar 31.
- Cytotechnology. 2026 Jun;78(3):116. [Abstract]
- Mol Biol. 2026 Mar 10.
- Res Sq. 2026 Jun 7.
- SSRN. 2025 Nov 26.
- Wayne State University. 2025.
- University of California, Irvine. 2024.
- Biomed Pharmacother. 2024 May:174:116500. [Abstract]
- Environ Toxicol. 2024 Apr;39(4):2363-2373. [Abstract]
- Biomed Pharmacother. 2023 May:161:114534. [Abstract]
- Research Square Print. 2023 Mar 22.
- Research Square Print. 2022 May.
- Bioengineered. 2022 May;13(5):11907-11922 [Abstract]
- Oxid Med Cell Longev. 2022 Jan 5:2022:7420507. [Abstract]
- Hypoxia. 2016 Nov 8:4:147-159. [Abstract]
Customer Validation & Images
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Histological Imaging/Staining
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Biological Activity
HIF-1α prolyl hydroxylase[1]
DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system[1]. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 89464-63-1
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Appearance Solid
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Molecular Weight 175.14
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Formula C6H9NO5
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Color White to off-white
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SMILES
O=C(OC)CNC(C(OC)=O)=O
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Synonyms
Dimethyloxallyl Glycine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (86)
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Journal Impact Factor
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Most Recent
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Cell Res
The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. [Abstract]2021 Sep;31(9):980-997. PMID: 34012073 -
Bioact Mater
Dimethyloxallyl glycine/nanosilicates-loaded osteogenic/angiogenic difunctional fibrous structure for functional periodontal tissue regeneration. [Abstract]2020 Oct 26;6(4):1175-1188. PMID: 33163699 -
Autophagy
Phase separation of OPTN initiates mitophagy to orchestrate craniofacial bone mineralization. [Abstract]2026 May;22(5):1021-1043. PMID: 41692957 -
Sci Immunol
2026 Apr 3;11(118):eaeb6484. PMID: 41931597 -
Redox Biol
Porcine epidemic diarrhea virus promotes viral replication via ROS/HIF-1α-mediated glycolysis. [Abstract]2026 Feb:89:104008. PMID: 41512444 -
Mol Cell
Longitudinal monitoring of cytoplasmic RBP-RNA interactions and transcriptome in living cells by engineered protein nanocages. [Abstract]2026 Jun 25:S1097-2765(26)00378-3. PMID: 42349405 -
Theranostics
2020 Jun 12;10(16):7409-7421. PMID: 32642002 -
Acta Pharm Sin B
PARylation promotes acute kidney injury via RACK1 dimerization-mediated HIF-1 α degradation. [Abstract]2025 Sep;15(9):4673-4691. PMID: 41049746 -
Adv Sci (Weinh)
Pyruvate Carboxylase in Macrophages Aggravates Atherosclerosis by Regulating Metabolism Reprogramming to Promote Inflammatory Responses Through the Hypoxia-Inducible Factor-1 Signaling Pathway. [Abstract]2025 Aug;12(29):e17128. PMID: 40391718
DMOG purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug;12(29):e17128. [Abstract]
Male PCMKO mice were administered with AAV-PCSK9DY, followed by HFD feeding for 12 weeks and injected with HIF-1 stabilizer DMOG (8 mg per mouse) or saline for the last 3 weeks (n = 8). BMDMs of PCMKO mice were collected for examination. Representative images and collagen quantification of the aortic roots stained with Masson staining were shown. Scale bars: 250 μm.
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Adv Sci (Weinh)
Neuraminidase 1 Exacerbated Glycolytic Dysregulation and Cardiotoxicity by Destabilizing SIRT1 through Interactions with NRF2 and HIF1α. [Abstract]2025 Jul;12(25):e2414504. PMID: 40411250
DMOG purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul;12(25):e2414504. [Abstract]
NRCMs were administered with 1 mM DMOG for 24 h, and the protein levels of NEU1 as well as HIF1α protein expression were measured by western blot.
DMOG purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul;12(25):e2414504. [Abstract]
NRCMs were administered with 1 μM DOX and/or 1 mM DMOG for 24 h, and the mRNA levels of NEU1 were measured by RT-qPCR (n = 3).
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Cell Rep Med
BCAT1-dependent HIF-1α stabilization is a targetable metabolic vulnerability in hepatocellular carcinoma. [Abstract]2026 Apr 29:102784. PMID: 42061407 -
Cell Death Differ
2026 Jun 24. PMID: 42337094 -
Biomaterials
Injectable immunomodulation-based porous chitosan microspheres/HPCH hydrogel composites as a controlled drug delivery system for osteochondral regeneration. [Abstract]2022 Jun;285:121530. PMID: 35504181 -
Cell Death Differ
CRL4DCAF8 dependent opposing stability control over the chromatin remodeler LSH orchestrates epigenetic dynamics in ferroptosis. [Abstract]2021 May;28(5):1593-1609. PMID: 33288900 -
Cell Death Dis
2026 Mar 30;17(1):430. PMID: 41912489 -
J Transl Med
Cholesterol suppresses human iTreg differentiation and nTreg function through mitochondria-related mechanisms. [Abstract]2023 Mar 27;21(1):224. PMID: 36973679 -
Chin Med J (Engl)
Kaempferide inhibited progression of osteoarthritis by targeting the HIF-1 signaling pathway. [Abstract]2025 Sep 17. PMID: 40960306 -
Oncogene
GPER-mediated stabilization of HIF-1α contributes to upregulated aerobic glycolysis in tamoxifen-resistant cells. [Abstract]2023 Jan;42(3):184-197. PMID: 36400971 -
BMC Med
Epigenetic drug screening for trophoblast syncytialization reveals a novel role for MLL1 in regulating fetoplacental growth. [Abstract]2024 Feb 5;22(1):57. PMID: 38317232 -
Cell Mol Gastroenterol Hepatol
A Critical Role for the Mitochondrial Pyruvate Carrier in Hepatic Stellate Cell Activation. [Abstract]2025;19(8):101517. PMID: 40239806 -
Cell Mol Gastroenterol Hepatol
2022;13(4):1057-1072. PMID: 34902628 -
Am J Chin Med
Cannabidiol Reprograms Glucose Metabolism in Colorectal Adenocarcinoma by Targeting HIF-1α/LDHA Pathway. [Abstract]2025;53(8):2561-2578. PMID: 41219135 -
ACS Appl Mater Interfaces
Multifunctional Electrospinning Polyhydroxyalkanoate Fibrous Scaffolds with Antibacterial and Angiogenesis Effects for Accelerating Wound Healing. [Abstract]2023 Jan 11;15(1):364-377. PMID: 36577512 -
Cell Rep
α-Ketoglutarate couples cellular metabolism to developmental growth via hydroxylation-dependent degradation of Yorkie. [Abstract]2026 Jan 28;45(2):116922. PMID: 41610010 -
Cell Rep
Metabolic disruption exacerbates intestinal damage during sleep deprivation by abolishing HIF1α-mediated repair. [Abstract]2024 Nov 10;43(11):114915. PMID: 39527478 -
Cell Rep
An oxygen-adaptive interaction between SNHG12 and occludin maintains blood-brain barrier integrity. [Abstract]2022 Apr 12;39(2):110656. PMID: 35417709 -
Br J Pharmacol
Inhibition of DNA methylation de-represses peroxisome proliferator-activated receptor-γ and attenuates pulmonary fibrosis. [Abstract]2022 Apr;179(7):1304-1318. PMID: 34378791 -
Front Immunol
Hypoxia Exacerbates Inflammatory Acute Lung Injury via the Toll-Like Receptor 4 Signaling Pathway. [Abstract]2018 Jul 23:9:1667. PMID: 30083155
DMOG purchased from MedChemExpress. Usage Cited in: Front Immunol. 2018 Jul 23:9:1667. [Abstract]
1 mM DMOG treatment for 8 h was used to upregulate the HIF-1α protein level in NR8383 cells.
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Redox Rep
Macrophage metabolic reprogramming via HIF-1α-glycolysis drives osteoblast ferroptosis and bone loss through an IL-6-STAT3-dependent redox axis. [Abstract]2026 Dec 31;31(1):2667673. PMID: 42071169 -
Antioxid Redox Signal
Targeting the HNRNPA2B1/HDGF/PTN Axis to Overcome Radioresistance in Non-Small Cell Lung Cancer. [Abstract]2025 Aug;43(4-6):189-214. PMID: 40643435 -
J Ethnopharmacol
Exploration of the underlying mechanism of Astragaloside III in attenuating immunosuppression via network pharmacology and vitro/vivo pharmacological validation. [Abstract]2024 Aug 10:330:118235. PMID: 38648891 -
Antioxid Redox Signal
Reversal of Epigenetic Peroxisome Proliferator-Activated Receptor-γ Suppression by Diacerein Alleviates Oxidative Stress and Osteoarthritis in Mice. [Abstract]2022 Jul;37(1-3):40-53. PMID: 35196878 -
Ecotoxicol Environ Saf
Manganese overexposure results in ferroptosis through the HIF-1α/p53/SLC7A11 pathway in ICR mouse brain and PC12 cells. [Abstract]2024 Jul 1:279:116481. PMID: 38788562 -
Cell Mol Life Sci
Exogenous acetate attenuates inflammatory responses through HIF-1α-dependent glycolysis regulation in macrophage. [Abstract]2024 Dec 27;82(1):21. PMID: 39725781 -
Cell Mol Life Sci
Uncovering the role of TET2-mediated ENPEP activation in trophoblast cell fate determination. [Abstract]2024 Jun 17;81(1):270. PMID: 38886218 -
Cell Mol Life Sci
An active glutamine/α-ketoglutarate/HIF-1α axis prevents pregnancy loss by triggering decidual IGF1+GDF15+NK cell differentiation. [Abstract]2022 Nov 30;79(12):611. PMID: 36449080 -
Biochem Pharmacol
Hydroquinone destabilizes BIM mRNA through upregulation of p62 in chronic myeloid leukemia cells. [Abstract]2022 May;199:115017. PMID: 35346662 -
Cancer Gene Ther
Nogo-B receptor increases glycolysis and the paclitaxel resistance of estrogen receptor-positive breast cancer via the HIF-1α-dependent pathway. [Abstract]2023 May;30(5):647-658. PMID: 36241702 -
Mol Metab
Proline hydroxylase 2 (PHD2) promotes brown adipose thermogenesis by enhancing the hydroxylation of UCP1. [Abstract]2023 Jul:73:101747. PMID: 37279828 -
Drug Des Devel Ther
Cinobufagin Inhibits Invasion and Migration of Non-Small Cell Lung Cancer via Regulating Glucose Metabolism Reprogramming in Tumor-Associated Macrophages. [Abstract]2025 Aug 2:19:6647-6664. PMID: 40771860 -
Eur J Pharmacol
Nobiletin ameliorates intervertebral disc degeneration by upregulating HIF-1α to inhibit endoplasmic reticulum stress-induced apoptosis. [Abstract]2026 Jan 12:1011:178480. PMID: 41397628 -
Cancer Biol Ther
HIF-1α inhibition by 2-methoxyestradiol induces cell death via activation of the mitochondrial apoptotic pathway in acute myeloid leukemia. [Abstract]2016 Jun 2;17(6):625-34. PMID: 27082496 -
Biomolecules
Hypoxia Regulates the Proliferation and Apoptosis of Coronary Artery Smooth Muscle Cells Through HIF-1α Mediated Autophagy in Yak. [Abstract]2025 Feb 10;15(2):256. PMID: 40001559 -
Mucosal Immunol
2022 Feb;15(2):268-278. PMID: 35013572 -
Arthritis Res Ther
2025 Sep 26;27(1):180. PMID: 41013704 -
Transl Oncol
Loss of the RAGE function in hypoxic conditions exacerbates the malignant progression of lung adenocarcinoma via damage-associated molecular pattern signaling. [Abstract]2026 Jun 18:71:102847. PMID: 42314516 -
Cell Signal
Regulatory effects of HIF-1α and HO-1 in hypoxia-induced proliferation of pulmonary arterial smooth muscle cells in yak. [Abstract]2021 Nov:87:110140. PMID: 34478827 -
Front Physiol
Notch3 Modulates Cardiac Fibroblast Proliferation, Apoptosis, and Fibroblast to Myofibroblast Transition via Negative Regulation of the RhoA/ROCK/Hif1α Axis. [Abstract]2020 Jun 30:11:669. PMID: 32695015 -
Clin Immunol
USP18 induction regulates immunometabolism to attenuate M1 signal-polarized macrophages and enhance IL-4-polarized macrophages in systemic lupus erythematosus. [Abstract]2024 Aug:265:110285. PMID: 38880201 -
Curr Issues Mol Biol
Dimethyloxalylglycine Suppresses SREBP1c and Lipogenic Gene Expressions in Hepatocytes Independently of HIF1A. [Abstract]2024 Mar 13;46(3):2386-2397. PMID: 38534767 -
Epigenomes
Modulation of DNA Methylation/Demethylation Reactions Induced by Nutraceuticals and Pollutants of Exposome Can Promote a C > T Mutation in the Breast Cancer Predisposing Gene PALB2. [Abstract]2022 Sep 30;6(4):32. PMID: 36278678 -
J Biol Chem
The tumor suppressor pVHL down-regulates never-in-mitosis A-related kinase 8 via hypoxia-inducible factors to maintain cilia in human renal cancer cells. [Abstract]2015 Jan 16;290(3):1389-94. PMID: 25451921
DMOG purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2015 Jan 16;290(3):1389-94. [Abstract]
The hypoxia induced the expression of NEK8. Cells are treated with CoCl2 (100 μM) or DMOG (100 μM) for 6 h or cultured for 12 h in hypoxia (1.0% oxygen). HIF-1α and NEK8 are analyzed by immunoblotting, GAPDH is employed as a loading control.
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Vet Res
Neospora caninum hijacks host PFKFB3-driven glycolysis to facilitate intracellular propagation of parasites. [Abstract]2025 Apr 30;56(1):94. PMID: 40307939 -
J Neurotrauma
Stabilization of Hypoxia Inducible Factor-1α by Dimethyloxalylglycine Promotes Recovery from Acute Spinal Cord Injury by Inhibiting Neural Apoptosis and Enhancing Axon Regeneration. [Abstract]2019 Dec 15;36(24):3394-3409. PMID: 31232175 -
Arch Med Res
Mechanism of SENP1-Mediated Regulation of Liver Sinusoidal Endothelial Cells to Promote Regeneration Via the HIF-1α Signaling Pathway. [Abstract]2025 Aug 22;56(8):103295. PMID: 40848695 -
J Dent Sci
Immunomodulatory effect of dimethyloxallyl glycine/nanosilicates-loaded fibrous structure on periodontal bone remodeling. [Abstract]2021 Jul;16(3):937-947. PMID: 34141108 -
Eur Cell Mater
The effect of hyaluronic acid on nucleus pulposus extracellular matrix production through hypoxia-inducible factor-1α transcriptional activation of CD44 under hypoxia. [Abstract]2021 Feb 3:41:142-152. PMID: 33533018 -
J Reprod Immunol
Immunomodulation of peripheral blood NK cells by sialic acid expressed on syncytialized BeWo cells. [Abstract]2026 Feb 11:174:104848. PMID: 41691902 -
J Cancer
EGLN1 induces tumorigenesis and radioresistance in nasopharyngeal carcinoma by promoting ubiquitination of p53 in a hydroxylase-dependent manner. [Abstract]2022 Mar 28;13(7):2061-2073. PMID: 35517429 -
Neuroscience
Imaging Hypoxic Stress and the Treatment of Amyotrophic Lateral Sclerosis with Dimethyloxalylglycine in a Mice Model. [Abstract]2019 Sep 1:415:31-43. PMID: 31344397 -
Tissue Cell
SENP1 drives glycolytic reprogramming to promote malignant progression of gastric cancer via deSUMOylation of HIF-1α. [Abstract]2026 Jun 17:103:103703. PMID: 42378852 -
Am J Cancer Res
SPATA20 deficiency enhances the metastatic and angiogenic potential of cancer cells by promoting HIF-1α synthesis. [Abstract]2024 Feb 15;14(2):727-743. PMID: 38455399 -
BMC Mol Cell Biol
mTOR signaling pathway regulation HIF-1 α effects on LPS induced intestinal mucosal epithelial model damage. [Abstract]2024 Apr 23;25(1):13. PMID: 38654163 -
Metallomics
2024 Oct 4;16(10):mfae044. PMID: 39317669 -
Acta Histochem
Inhibitor of growth 4 (ING4) inhibits hypoxia-induced EMT by decreasing HIF-1α and snail in HK2 cells. [Abstract]2019 Aug;121(6):695-703. PMID: 31239073 -
Reprod Sci
2023 Mar;30(3):928-951. PMID: 36042151 -
PLoS One
The protective effects of HIF-1α activation on sepsis induced intestinal mucosal barrier injury in rats model of sepsis. [Abstract]2022 May 16;17(5):e0268445. PMID: 35576220 -
Gene
HIF signaling overactivation inhibits lateral line neuromast development through Wnt in zebrafish. [Abstract]2024 Mar 10:898:148077. PMID: 38097093 -
Thorac Cancer
Mitochondrial NDUFA4L2 protein promotes the vitality of lung cancer cells by repressing oxidative stress. [Abstract]2019 Apr;10(4):676-685. PMID: 30710412 -
Biochem Biophys Res Commun
VHL regulates NEK1 via both HIF-2α pathway and ubiquitin-proteasome pathway in renal cancer cell. [Abstract]2019 Feb 12;509(3):797-802. PMID: 30635121 -
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Cytotechnology
PX-12 exhibits cytotoxic and anti-migration effects in colorectal cancer cells under Hypoxia-like conditions induced by Dimethyloxalylglycine. [Abstract]2026 Jun;78(3):116. PMID: 42145842 -
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Biomed Pharmacother
Chrysin inhibits ferroptosis of cerebral ischemia/reperfusion injury via regulating HIF-1α/CP loop. [Abstract]2024 May:174:116500. PMID: 38555815 -
Environ Toxicol
2024 Apr;39(4):2363-2373. PMID: 38156404 -
Biomed Pharmacother
Chrysin protects against cerebral ischemia-reperfusion injury in hippocampus via restraining oxidative stress and transition elements. [Abstract]2023 May:161:114534. PMID: 36933376 -
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Bioengineered
β-Patchoulene represses hypoxia-induced proliferation and epithelial-mesenchymal transition of liver cancer cells. [Abstract]2022 May;13(5):11907-11922 PMID: 35546067 -
Oxid Med Cell Longev
Celastrol Protects against Cerebral Ischemia/Reperfusion Injury in Mice by Inhibiting Glycolysis through Targeting HIF-1 α/PDK1 Axis. [Abstract]2022 Jan 5:2022:7420507. PMID: 35035665 -
Hypoxia
2016 Nov 8:4:147-159. PMID: 27878135
Solvent & Solubility
DMSO : 125 mg/mL (713.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (285.49 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 150 mg/mL (856.46 mM); Clear solution; Need ultrasonic
Protocol
To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Two groups of mice (C57Bl6) are used. One group (n=11) receives dimethyloxalylglycine (DMOG) i.p. (8 mg in 0.5 mL saline) on days 1, 3, 5, 7 and 9 while the animals in the other group are injected with sterile saline (0.5 mL) at the same intervals (n=6). A third group is treated with DMOG without ligation (n=4) and four unoperated mice serve as controls. After 11 days mice are terminally anaesthetized and the extensor digitorum longus (EDL) and tibialis anterior (TA) muscles excised.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Baader E, et al. Inhibition of prolyl 4-hydroxylase by oxalyl amino acid derivatives in vitro, in isolated microsomes and in embryonic chicken tissues. Biochem J. 1994 Jun 1;300 (Pt 2):525-30. [Content Brief]
[2]. Milkiewicz M, et al. Inhibition of endogenous HIF inactivation induces angiogenesis in ischaemic skeletal muscles of mice. J Physiol. 2004 Oct 1;560(Pt 1):21-6. [Content Brief]
[3]. Schultz K, et al. Prolyl hydroxylase 2 deficiency limits proliferation of vascular smooth muscle cells by hypoxia-inducible factor-1{alpha}-dependent mechanisms. Am J Physiol Lung Cell Mol Physiol. 2009 Jun;296(6):L921-7. [Content Brief]
[4]. Li X, et al. Up-regulation of hypoxia-inducible factor-1α enhanced the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):112-8. [Content Brief]
[5]. Singh A, et al. Hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitors induce autophagy and have a protective effect in an in-vitro ischaemia model.Sci Rep. 2020 Jan 31;10(1):1597. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.7097 mL | 28.5486 mL | 57.0972 mL | 142.7429 mL |
| 5 mM | 1.1419 mL | 5.7097 mL | 11.4194 mL | 28.5486 mL | |
| 10 mM | 0.5710 mL | 2.8549 mL | 5.7097 mL | 14.2743 mL | |
| 15 mM | 0.3806 mL | 1.9032 mL | 3.8065 mL | 9.5162 mL | |
| 20 mM | 0.2855 mL | 1.4274 mL | 2.8549 mL | 7.1371 mL | |
| 25 mM | 0.2284 mL | 1.1419 mL | 2.2839 mL | 5.7097 mL | |
| 30 mM | 0.1903 mL | 0.9516 mL | 1.9032 mL | 4.7581 mL | |
| 40 mM | 0.1427 mL | 0.7137 mL | 1.4274 mL | 3.5686 mL | |
| 50 mM | 0.1142 mL | 0.5710 mL | 1.1419 mL | 2.8549 mL | |
| 60 mM | 0.0952 mL | 0.4758 mL | 0.9516 mL | 2.3790 mL | |
| 80 mM | 0.0714 mL | 0.3569 mL | 0.7137 mL | 1.7843 mL | |
| 100 mM | 0.0571 mL | 0.2855 mL | 0.5710 mL | 1.4274 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DMOG Related Classifications
HY-15893 Related Classifications
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