Sennoside A
Based on 6 publication(s) in Google Scholar
Sennoside A is an anthraquinone glycoside found in senna (Cassia angustifolia). Sennoside A is an HIV-1 inhibitor (IC50=3.8 μM) that inhibits HIV-1 replication. Sennoside A also inhibits HIV-1 reverse transcriptase (RT)-related DNA polymerase (RDDP) and ribonuclease H (Ribonuclease H) with IC50s of 1.9 μM and 5.3 μM, respectively.
For research use only. We do not sell to patients.
- Purity: 99.66%
- CAS No.: 81-27-6
- Formula: C42H38O20
- Molecular Weight:862.74
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Sennoside A
More- Int Immunopharmacol. 2023 Jul:120:110290. [Abstract]
- J Enzyme Inhib Med Chem. 2025 Dec;40(1):2501743. [Abstract]
- Naunyn Schmiedebergs Arch Pharmacol. 2025 May;398(5):5405-5415. [Abstract]
- Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- J Mol Histol. 2025 Dec 29;57(1):15. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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IF
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ELISA
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Histological Imaging/Staining
All DNA/RNA Synthesis Isoforms
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Biological Activity
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HIV-1 |
DNA Polymerase |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
68.2 μM
Compound: 7
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Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
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[PMID: 17949858] |
| HepG2 | IC50 |
51.6 μM
Compound: 7
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Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
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[PMID: 17949858] |
Sennoside A inhibits different variants of RDDP and RNase H. Inhibits different variants of RDDP with IC50s of 78 μM (K103N RT), 21.3 μM (Y181C RT), and 64 μM (Y188L RT), respectively. Inhibits different variants of RNase H with IC50s of 18.4 μM (N474A RT) and 17.7 μM (Q475A RT), respectively[3].
Infects Jurka cells with HIV-1 recombinant CAT virus, which is pseudotyped with the envelope glycoprotein from the HXBc2 laboratory-adapted T-tropic virus. Sennoside A (5-20 μM; 72 h) significantly inhibits CAT activity in infected cell[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Sennoside A also reduces inflammation and increases tight junction proteins in the ileum of genetically defective mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 81-27-6
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Appearance Solid
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Molecular Weight 862.74
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Formula C42H38O20
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Color Light yellow to yellow
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SMILES
O=C(C(C=C1[C@@]([C@]2([H])C3=C(C(O[C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)=CC=C3)C(C5=C(O)C=C(C(O)=O)C=C25)=O)([H])C6=C7C(O[C@H]8[C@@H]([C@H]([C@@H]([C@@H](CO)O8)O)O)O)=CC=C6)=CC(O)=C1C7=O)O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (6)
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Journal Impact Factor
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Most Recent
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Int Immunopharmacol
Sennoside A restrains TRAF6 level to modulate ferroptosis, inflammation and cognitive impairment in aging mice with Alzheimer's Disease. [Abstract]2023 Jul:120:110290. PMID: 37216800 -
J Enzyme Inhib Med Chem
Natural product sennoside B disrupts liquid-liquid phase separation of SARS-CoV-2 nucleocapsid protein by inhibiting its RNA-binding activity. [Abstract]2025 Dec;40(1):2501743. PMID: 40371698 -
Naunyn Schmiedebergs Arch Pharmacol
Sennoside A represses the malignant phenotype and tumor immune microenvironment of non-small cell lung cancer cells by inhibiting the TRAF6/NF-κB pathway. [Abstract]2025 May;398(5):5405-5415. PMID: 39549059 -
Integr Cancer Ther
Sennoside A Modulates the Ferroptosis and Immune Evasion of Oral Squamous Cell Carcinoma Cells Through Inhibiting the NF-κB Pathway. [Abstract]2025 Jan-Dec:24:15347354251375464. PMID: 40958203
Sennoside A purchased from MedChemExpress. Usage Cited in: Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464. [Abstract]
The cell viability was detected by CCK-8 assays after HOK cells were treated with 0, 6.25, 12.5, 25, 50, 100, 200 and 400 μM of Sennoside A (SA) for 48 hours.
Sennoside A purchased from MedChemExpress. Usage Cited in: Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464. [Abstract]
The relative protein expression of GPX4 and xCT was examined by western blot after SCC7 and CAL27 cells were treated with 25, 50 and 100 μM Sennoside A (SA) for 48 hours.
Sennoside A purchased from MedChemExpress. Usage Cited in: Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464. [Abstract]
The relative lipid ROS level was examined after SCC7 and CAL27 cells were incubated with 25, 50 and 100 μM Sennoside A (SA) for 48 hours.
Sennoside A purchased from MedChemExpress. Usage Cited in: Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464. [Abstract]
Measurement of IFN-γ, IL-2 and TNF-α by ELISA incubated with 25, 50 and 100 μM Sennoside A (SA).
Sennoside A purchased from MedChemExpress. Usage Cited in: Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464. [Abstract]
Pathological examination by HE staining, and the expression levels of GPX4, PD-L1, and IFN-γ in tumor tissues were detected by immunohistochemistry treated with Sennoside A (SA) (10 mg/kg, i.p.).
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Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
J Mol Histol
Sennoside A alleviates sepsis-triggered acute kidney injury via activating SIRT1 signaling pathway. [Abstract]2025 Dec 29;57(1):15. PMID: 41460380
Solvent & Solubility
DMSO : 100 mg/mL (115.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 6.25 mg/mL (7.24 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 6.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Rama Reddy NR, et al. Next Generation Sequencing and Transcriptome Analysis Predicts Biosynthetic Pathway of Sennosides from Senna (Cassia angustifolia Vahl.), a Non-Model Plant with Potent Laxative Properties. PLoS One. 2015 Jun 22;10(6):e0129422. [Content Brief]
[2]. Esposito F, et al. Sennoside A, derived from the traditional chinese medicine plant Rheum L., is a new dual HIV-1 inhibitor effective on HIV-1 replication. Phytomedicine. 2016 Nov 15;23(12):1383-1391. [Content Brief]
[3]. Esposito F, et al. Sennoside A, derived from the traditional chinese medicine plant Rheum L., is a new dual HIV-1 inhibitor effective on HIV-1 replication. Phytomedicine. 2016 Nov 15;23(12):1383-1391. [Content Brief]
[4]. Wei Z, et al. Gut Bacteria Selectively Altered by Sennoside A Alleviate Type 2 Diabetes and Obesity Traits. Oxid Med Cell Longev. 2020 Jun 25;2020:2375676. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1591 mL | 5.7955 mL | 11.5910 mL | 28.9774 mL |
| 5 mM | 0.2318 mL | 1.1591 mL | 2.3182 mL | 5.7955 mL | |
| 10 mM | 0.1159 mL | 0.5795 mL | 1.1591 mL | 2.8977 mL | |
| 15 mM | 0.0773 mL | 0.3864 mL | 0.7727 mL | 1.9318 mL | |
| 20 mM | 0.0580 mL | 0.2898 mL | 0.5795 mL | 1.4489 mL | |
| 25 mM | 0.0464 mL | 0.2318 mL | 0.4636 mL | 1.1591 mL | |
| 30 mM | 0.0386 mL | 0.1932 mL | 0.3864 mL | 0.9659 mL | |
| 40 mM | 0.0290 mL | 0.1449 mL | 0.2898 mL | 0.7244 mL | |
| 50 mM | 0.0232 mL | 0.1159 mL | 0.2318 mL | 0.5795 mL | |
| 60 mM | 0.0193 mL | 0.0966 mL | 0.1932 mL | 0.4830 mL | |
| 80 mM | 0.0145 mL | 0.0724 mL | 0.1449 mL | 0.3622 mL | |
| 100 mM | 0.0116 mL | 0.0580 mL | 0.1159 mL | 0.2898 mL |
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