YTHDF2-IN-1
Based on 1 Customer Validation
YTHDF2-IN-1 (Compound CK-75) is a selective YTHDF2 inhibitor (Kd = 26.2 μM). YTHDF2-IN-1 binds to a small hydrophobic pocket on the YTH domain of YTHDF2, disrupting the interaction between YTHDF2 and m6A-modified RNA. YTHDF2-IN-1 induces cell cycle arrest and Apoptosis. YTHDF2-IN-1 is applicable to research on chronic myeloid leukemia, colon cancer and choriocarcinoma.
For research use only. We do not sell to patients.
- Purity: 99.42%
- Formula: C21H14N2O4
- Molecular Weight:358.35
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Epigenetics Compound Library
HY-L005
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Anti-Cancer Compound Library
HY-L025
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Anti-Colorectal Cancer Compound Library
HY-L103
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Highly Selective Inhibitors Library
HY-L158
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Highly Selective Activators Library
HY-L159
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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Epigenetics Compound Library Mini
HY-L005M
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Protein Kinase Compound Library
HY-L196
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
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YTHDF2 26.2 μM (Kd) |
YTHDF2-IN-1 (100 μM; 30 min) inhibits the interaction between the purified YTHDF2 YTH domain and m6A RNA in FP assays, with an inhibition rate of 82.3% at a concentration of 100 μM[1].
YTHDF2-IN-1 binds to the purified YTH domain of YTHDF2 in MST assays, with a Kd value of 26.2 μM[1].
YTHDF2-IN-1 (10-25 μM; 24 h) induces concentration-dependent G0/G1 cell cycle arrest in K562 cells[1].
YTHDF2-IN-1 (25-100 μM; 24 h) induces concentration-dependent apoptosis in K562 cells[1].
YTHDF2-IN-1 (0.4-100 μM; 72 h) exhibits micromolar cytotoxicity against K562, HCT116 and JAR cancer cells in MTT assays, with corresponding IC50 values of 29.7 μM, 33.3 μM and 47.5 μM after 72 h of treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:K562 human cancer cells
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Concentration:10-25 μM
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Incubation Time:24 h
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Result:Caused >70% of cells to accumulate in the G0/G1 phase at 25 μM.
Induced concentration-dependent G0/G1 phase cell cycle arrest.
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Cell Line:K562 human cancer cells
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Concentration:25, 50, 100 μM
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Incubation Time:24 h
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Result:Induced total apoptosis in 28.2% of cells at 25 μM, 34.9% at 50 μM, and 45.4% at 100 μM.
Chemical Information
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Appearance Solid
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Molecular Weight 358.35
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Formula C21H14N2O4
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Color Yellow to orange
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SMILES
O=C(O)C1=CC=C(N2N=CC(C(C3=C4C=CC=CC4=CC=C3O)=O)=C2)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (139.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7906 mL | 13.9528 mL | 27.9057 mL | 69.7642 mL |
| 5 mM | 0.5581 mL | 2.7906 mL | 5.5811 mL | 13.9528 mL | |
| 10 mM | 0.2791 mL | 1.3953 mL | 2.7906 mL | 6.9764 mL | |
| 15 mM | 0.1860 mL | 0.9302 mL | 1.8604 mL | 4.6509 mL | |
| 20 mM | 0.1395 mL | 0.6976 mL | 1.3953 mL | 3.4882 mL | |
| 25 mM | 0.1116 mL | 0.5581 mL | 1.1162 mL | 2.7906 mL | |
| 30 mM | 0.0930 mL | 0.4651 mL | 0.9302 mL | 2.3255 mL | |
| 40 mM | 0.0698 mL | 0.3488 mL | 0.6976 mL | 1.7441 mL | |
| 50 mM | 0.0558 mL | 0.2791 mL | 0.5581 mL | 1.3953 mL | |
| 60 mM | 0.0465 mL | 0.2325 mL | 0.4651 mL | 1.1627 mL | |
| 80 mM | 0.0349 mL | 0.1744 mL | 0.3488 mL | 0.8721 mL | |
| 100 mM | 0.0279 mL | 0.1395 mL | 0.2791 mL | 0.6976 mL |
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