3-Pentanol
Based on 1 Customer Validation
3-Pentanol is an active organic compound produced by plants and is a component of insect-released pheromones. 3-Pentanol can trigger plant immunity against microbial pathogens and pests in crops by activating the SA and JA signaling pathways.
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- Pureza: 99.76%
- No. CAS: 584-02-1
- Fòrmula: C5H12O
- Peso molecular:88.15
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Almacenamiento:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Actividad biológica
3-pentanol (100 nM, 7 days) activates SA and JA signaling pathways, promoting expression of immune-related genes in Arabidopsis thaliana[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Leaf tissue of Arabidopsis thaliana
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Concentration:100 nM
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Incubation Time:7 days
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Result:Significantly upregulated PDF1.2 and PR1 gene transcription levels.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Arabidopsis thaliana
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Dosage:100 nM and 10 µM
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Administration:Administered via gaseous exposure using the I-plate system (Petri dish divided into two separated compartments), exposed for 7 days, followed by pathogen inoculation
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Result:Significantly reduced disease severity in Arabidopsis thaliana.
Chemical Information
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No. CAS 584-02-1
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Appearance Liquid (Density: 0.8 g/cm3)
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Peso molecular 88.15
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Fòrmula C5H12O
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Color Colorless to light yellow
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SMILES
CCC(O)CC
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Structure Classification
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Initial Source
the ambrosia beetle, Megaplatypus mutatus
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (1134.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (28.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (28.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (271 KB)
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SDS (605 KB)
- English - EN (605 KB)
- Français - FR (605 KB)
- Deutsch - DE (605 KB)
- Norwegian - NO (605 KB)
- Español - ES (605 KB)
- Swedish - SV (605 KB)
- Italian - IT (605 KB)
- Korean - KR (605 KB)
- Portuguese - PT (605 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Geun C Song, et al. Gaseous 3-pentanol primes plant immunity against a bacterial speck pathogen, Pseudomonas syringae pv. tomato via salicylic acid and jasmonic acid-dependent signaling pathways in Arabidopsis. Front Plant Sci. 2015 Oct 6;6:821. [Content Brief]
[2]. Song GC, et al. Gaseous 3-pentanol primes plant immunity against a bacterial speck pathogen, Pseudomonas syringae pv. tomato via salicylic acid and jasmonic acid-dependent signaling pathways in Arabidopsis. Front Plant Sci. 2015 Oct 6;6:821. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 11.3443 mL | 56.7215 mL | 113.4430 mL | 283.6075 mL |
| 5 mM | 2.2689 mL | 11.3443 mL | 22.6886 mL | 56.7215 mL | |
| 10 mM | 1.1344 mL | 5.6721 mL | 11.3443 mL | 28.3607 mL | |
| 15 mM | 0.7563 mL | 3.7814 mL | 7.5629 mL | 18.9072 mL | |
| 20 mM | 0.5672 mL | 2.8361 mL | 5.6721 mL | 14.1804 mL | |
| 25 mM | 0.4538 mL | 2.2689 mL | 4.5377 mL | 11.3443 mL | |
| 30 mM | 0.3781 mL | 1.8907 mL | 3.7814 mL | 9.4536 mL | |
| 40 mM | 0.2836 mL | 1.4180 mL | 2.8361 mL | 7.0902 mL | |
| 50 mM | 0.2269 mL | 1.1344 mL | 2.2689 mL | 5.6721 mL | |
| 60 mM | 0.1891 mL | 0.9454 mL | 1.8907 mL | 4.7268 mL | |
| 80 mM | 0.1418 mL | 0.7090 mL | 1.4180 mL | 3.5451 mL | |
| 100 mM | 0.1134 mL | 0.5672 mL | 1.1344 mL | 2.8361 mL |
3-Pentanol Related Classifications
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