Bornesitol
Bornesitol is a natural cytotoxic agent found in Michelia compressa var. lanyuensis. Bornesitol exhibits cytotoxic and antiproliferative activities, and can be used in melanoma research.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- CAS No.: 484-71-9
- Formule: C7H14O6
- Masse moléculaire:194.18
-
Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
- Protocole
- Pureté et documentation
- Références
-
Help & FAQs
Activité biologique
Description
In Vitro
Bornesitol (1-100 μM; 24 h) shows no cytotoxicity against B16F10 melanoma cells at concentrations of 1 μM or 10 μM after 24 h of incubation, but induces cytotoxicity at a concentration of 100 μM[1].
Bornesitol (5 μM; 48 h) weakly inhibits tyrosinase activity in B16F10 melanoma cells after incubation at 5 μM for 48 h, with an inhibition rate of 3.06%[1].
Bornesitol (5 μM; 48 h) exerts only an extremely weak inhibitory effect on melanin content in B16F10 melanoma cells, with an inhibition rate of 0.89% after 48 h of incubation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
-
Cell Line:B16F10 melanoma cells
-
Concentration:1 μM; 10 μM; 100 μM
-
Incubation Time:24 h
-
Result:Did not reduce B16F10 cell viability relative to vehicle control at 1 μM and 10 μM.
Caused cytotoxicity, reducing cell viability relative to vehicle control at 100 μM.
Chemical Information
-
CAS No. 484-71-9
-
Masse moléculaire 194.18
-
Formule C7H14O6
-
SMILES
O(C)[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O
-
Structure Classification
-
Initial Source
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Bornesitol Related Classifications
HY-N10575 Related Classifications
Powered by Bioz