7ACC1
Based on 10 publication(s) in Google Scholar
7ACC1 (DEAC; Coumarin D 1421; D 1421) is a monocarboxylate transporter 1 (MCT-1)/MCT-4 specifc blocker. 7ACC1 attenuates renal cancer cell proliferation, migration, invasion and down-regulates the levels of MCT1/MCT4 expression and extracellular lactate. 7ACC1 is promising for research of cancers.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.94%
- No. CAS: 50995-74-9
- Fòrmula: C14H15NO4
- Peso molecular:261.27
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 7ACC1
More- Cell Metab. 2021 Oct 5;33(10):2021-2039.e8. [Abstract]
- Microbiome. 2022 Dec 15;10(1):226. [Abstract]
- Cancer Cell Int. 2019 Jun 28:19:170. [Abstract]
- J Biol Chem. 2022 Feb;298(2):101554. [Abstract]
- Exp Cell Res. 2022 Mar 15;412(2):113044. [Abstract]
- Cancer Med. 2018 Sep;7(9):4690-4700. [Abstract]
- Aesthetic Plast Surg. 2024 Dec;48(23):5136-5146. [Abstract]
- bioRxiv. 2026 Mar 10.
- J Oncol. 2022 Sep 26;2022:3659714. [Abstract]
- Research Square Preprint. 2021 Aug.
Customer Validation & Images
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WB
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In Vivo Efficacy Study
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Cell Migration/Invasion Assay
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Cell Imaging/Staining
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WB
Actividad biológica
Descripciòn
IC50 & Target
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MCT1 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 4T1 | IC50 |
>100 μM
Compound: 7
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Antiproliferative activity against mouse 4T1 cells assessed as inhibition of cell proliferation by MTT assay
Antiproliferative activity against mouse 4T1 cells assessed as inhibition of cell proliferation by MTT assay
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[PMID: 34626786] |
| HepG2 | IC50 |
>100 μM
Compound: 10
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Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
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[PMID: 28666736] |
| MCF-10A | IC50 |
>100 μM
Compound: 7
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Cytotoxicity against human MCF-10A cells assessed as inhibition of cell proliferation by MTT assay
Cytotoxicity against human MCF-10A cells assessed as inhibition of cell proliferation by MTT assay
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[PMID: 34626786] |
| MCF7 | IC50 |
>100 μM
Compound: 10
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Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
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[PMID: 28666736] |
| MDA-MB-231 | IC50 |
>100 μM
Compound: 10
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Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay
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[PMID: 28666736] |
| MDA-MB-231 | IC50 |
>100 μM
Compound: 7
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Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation by MTT assay
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation by MTT assay
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[PMID: 34626786] |
| MDA-MB-231 | IC50 |
>50 μM
Compound: 11
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Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
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[PMID: 37602711] |
| SiHa | EC50 |
1.8 μM
Compound: 17
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Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay
Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay
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[PMID: 24095010] |
| SiHa | IC50 |
0.86 μM
Compound: 17
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Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting
Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting
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[PMID: 24095010] |
| SiHa | IC50 |
250 nM
Compound: 17
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Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 12 mins by liquid scintillation counting
Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 12 mins by liquid scintillation counting
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[PMID: 24095010] |
| SK-HEP1 | IC50 |
>100 μM
Compound: 10
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Antiproliferative activity against human SKHEP1 cells after 48 hrs by MTT assay
Antiproliferative activity against human SKHEP1 cells after 48 hrs by MTT assay
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[PMID: 28666736] |
| WiDr | IC50 |
>100 μM
Compound: 7
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Antiproliferative activity against human WiDr cells assessed as inhibition of cell proliferation by MTT assay
Antiproliferative activity against human WiDr cells assessed as inhibition of cell proliferation by MTT assay
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[PMID: 34626786] |
In Vitro
7ACC1 (10 μM, 0-96 h) attenuates the viability, decreases cell migration and invasion in co-culturing conditions of 786-O cells and HUVECs[1].
7ACC1 (10 μM, 24 h) inhibits the protein expressions of MCT1 and MCT4, down-regulates the extracellular lactate levels in co-culturing conditions of 786-O cells and HUVECs[1].
7ACC1 (0-1 μM, 24 h) inhibit the influx but not the efflux of lactate in cancer cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:786-O cells and HUVECs
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Concentration:10 µM
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Incubation Time:24 h
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Result:Attenuated the protein expressions of MCT1 and MCT4 in co-culturing of 786-O cells and HUVECs.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:mice bearing tumors, including cervix cancer SiHa xenografts but also tumors derived from human colon cancer cells HCT116 and bladder cancer cells UM-UC3[2]
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Dosage:3 mg/kg
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Administration:i.p., daily for 12 days
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Result:Led to significant SiHa and HCT116 tumor growth delays in mice bearing tumors.
- RNA extraction experimental
- BrdU Incorporation Assay
- Cell migration
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Cell invasion
- MTT Cell Proliferation Assay
Emission (Em)
445
Excitation (Ex)
345
Chemical Information
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No. CAS 50995-74-9
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Appearance Solid
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Peso molecular 261.27
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Fòrmula C14H15NO4
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Color Orange to red
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SMILES
O=C(C1=CC2=CC=C(N(CC)CC)C=C2OC1=O)O
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Synonyms
DEAC; Coumarin D 1421; D 1421
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (10)
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Journal Impact Factor
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Most Recent
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Cell Metab
The thermogenic activity of adjacent adipocytes fuels the progression of ccRCC and compromises anti-tumor therapeutic efficacy. [Abstract]2021 Oct 5;33(10):2021-2039.e8. PMID: 34508696 -
Microbiome
Limosilactobacillus reuteri and caffeoylquinic acid synergistically promote adipose browning and ameliorate obesity-associated disorders. [Abstract]2022 Dec 15;10(1):226. PMID: 36517893
7ACC1 purchased from MedChemExpress. Usage Cited in: Microbiome. 2022 Dec 15;10(1):226. [Abstract]
In parallel, along with the absence of cold resistance and browning of iWAT by 7ACC1 intervention, the increasing trend of MCT1 expression was also diminished.
7ACC1 purchased from MedChemExpress. Usage Cited in: Microbiome. 2022 Dec 15;10(1):226. [Abstract]
In parallel, along with the absence of cold resistance and browning of iWAT by 7ACC1 intervention, the increasing trend of MCT1 expression was also diminished.
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Cancer Cell Int
Monocarboxylate transporter 1 and monocarboxylate transporter 4 in cancer-endothelial co-culturing microenvironments promote proliferation, migration, and invasion of renal cancer cells. [Abstract]2019 Jun 28:19:170. PMID: 31297034
7ACC1 purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2019 Jun 28:19:170. [Abstract]
At 24 h after culturing, both 786-O cells and HUVECs showed better healing in co-culturing mode than that in single-culturing mode. Blocking MCT1 and MCT4 by supplementation of 7ACC1 (10 μM) in the culture medium markedly decreased migration of both 786-O cells and HUVECs in co-culturing mode, but it did not affect migration of cells in the single-culturing mode.
7ACC1 purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2019 Jun 28:19:170. [Abstract]
In the transwell chamber invasion assay, 786-O cells at a density of 1 × 104 cells/well were seeded in the upper chamber in serum-free medium supplemented with or without 10 µM 7ACC1, while 786-O cells or HUVECs at a density of 4 × 104 cells/well were seeded in the lower chamber in complete culture medium. 24 h after culturing, the 786-O cells that penetrated the membrane were counted under a microscope. Representative images show the membrane-invaded 786-O cells (magnification of ×200).
7ACC1 purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2019 Jun 28:19:170. [Abstract]
At 24 h after incubation with the MCTs blocker 7ACC1 (10 μM), the protein expressions of MCT1 and MCT4 were attenuated to the similar levels as that in single culturing condition.
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J Biol Chem
Mitochondrial pyruvate carrier inhibitors improve metabolic parameters in diet-induced obese mice. [Abstract]2022 Feb;298(2):101554. PMID: 34973337 -
Exp Cell Res
ZEB1 induces ROS generation through directly promoting MCT4 transcription to facilitate breast cancer. [Abstract]2022 Mar 15;412(2):113044. PMID: 35093305 -
Cancer Med
Downregulation of MCT4 for lactate exchange promotes the cytotoxicity of NK cells in breast carcinoma. [Abstract]2018 Sep;7(9):4690-4700. PMID: 30051648
7ACC1 purchased from MedChemExpress. Usage Cited in: Cancer Med. 2018 Sep;7(9):4690-4700. [Abstract]
The protein expression of MCT4 in the murine breast cancer cell line 4T1 treated with 7ACC1 (0.1 mM) or transfected with different ShMCT4 vectors.
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Aesthetic Plast Surg
Poly-L-Lactic Acid Reduces the Volume of Dermal Adipose Tissue Through its Metabolite Lactate. [Abstract]2024 Dec;48(23):5136-5146. PMID: 39060798 -
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J Oncol
2022 Sep 26;2022:3659714. PMID: 36199799 -
Solvente y solubilidad
In Vitro:
DMSO : 33.33 mg/mL (127.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Guo C, et al. Monocarboxylate transporter 1 and monocarboxylate transporter 4 in cancer-endothelial co-culturing microenvironments promote proliferation, migration, and invasion of renal cancer cells. Cancer Cell Int. 2019 Jun 28;19:170. [Content Brief]
[2]. Draoui N, et al. Antitumor activity of 7-aminocarboxycoumarin derivatives, a new class of potent inhibitors of lactate influx but not efflux. Mol Cancer Ther. 2014 Jun;13(6):1410-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8275 mL | 19.1373 mL | 38.2746 mL | 95.6865 mL |
| 5 mM | 0.7655 mL | 3.8275 mL | 7.6549 mL | 19.1373 mL | |
| 10 mM | 0.3827 mL | 1.9137 mL | 3.8275 mL | 9.5686 mL | |
| 15 mM | 0.2552 mL | 1.2758 mL | 2.5516 mL | 6.3791 mL | |
| 20 mM | 0.1914 mL | 0.9569 mL | 1.9137 mL | 4.7843 mL | |
| 25 mM | 0.1531 mL | 0.7655 mL | 1.5310 mL | 3.8275 mL | |
| 30 mM | 0.1276 mL | 0.6379 mL | 1.2758 mL | 3.1895 mL | |
| 40 mM | 0.0957 mL | 0.4784 mL | 0.9569 mL | 2.3922 mL | |
| 50 mM | 0.0765 mL | 0.3827 mL | 0.7655 mL | 1.9137 mL | |
| 60 mM | 0.0638 mL | 0.3190 mL | 0.6379 mL | 1.5948 mL | |
| 80 mM | 0.0478 mL | 0.2392 mL | 0.4784 mL | 1.1961 mL | |
| 100 mM | 0.0383 mL | 0.1914 mL | 0.3827 mL | 0.9569 mL |
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