M1001
Based on 1 Customer Validation
M1001 is a weak hypoxia-inducible factor-2α (HIF-2α) agonist. M1001 can bind to the HIF-2α PAS-B domain, with a Kd of 667 nM. M1001 can be used in chronic kidney disease research.
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- Pureza: 99.51%
- No. CAS: 874590-32-6
- Fòrmula: C17H17N3O2S
- Peso molecular:327.40
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Kd: 667 nM (HIF-2α)[1]
M1001 (10 μM) treatment shows increased expression of HIF-2 target genes in 786-O cells[1].
M1001 (10 μM) binds to HIF-2α and has the properties of a weak agonist[1].
M1001 (1-10 μM) reduces VHL binding to HIF-2α[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:786-O cells
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Concentration:10 µM
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Incubation Time:
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Result:Increased the expression of HIF-2 target genes modestly in 786-O cells.
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Cell Line:HEK293T cells
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Concentration:1 and 10 µM
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Incubation Time:
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Result:Reduced the physical association between HIF-2α and VHL.
Chemical Information
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No. CAS 874590-32-6
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Appearance Solid
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Peso molecular 327.40
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Fòrmula C17H17N3O2S
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Color Light green to green
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SMILES
O=S1(C2=C(C=CC=C2)C(NC3=C(N4CCCC4)C=CC=C3)=N1)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvente y solubilidad
DMSO : 250 mg/mL (763.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.33 mg/mL (7.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.33 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (23.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0544 mL | 15.2718 mL | 30.5437 mL | 76.3592 mL |
| 5 mM | 0.6109 mL | 3.0544 mL | 6.1087 mL | 15.2718 mL | |
| 10 mM | 0.3054 mL | 1.5272 mL | 3.0544 mL | 7.6359 mL | |
| 15 mM | 0.2036 mL | 1.0181 mL | 2.0362 mL | 5.0906 mL | |
| 20 mM | 0.1527 mL | 0.7636 mL | 1.5272 mL | 3.8180 mL | |
| 25 mM | 0.1222 mL | 0.6109 mL | 1.2217 mL | 3.0544 mL | |
| 30 mM | 0.1018 mL | 0.5091 mL | 1.0181 mL | 2.5453 mL | |
| 40 mM | 0.0764 mL | 0.3818 mL | 0.7636 mL | 1.9090 mL | |
| 50 mM | 0.0611 mL | 0.3054 mL | 0.6109 mL | 1.5272 mL | |
| 60 mM | 0.0509 mL | 0.2545 mL | 0.5091 mL | 1.2727 mL | |
| 80 mM | 0.0382 mL | 0.1909 mL | 0.3818 mL | 0.9545 mL | |
| 100 mM | 0.0305 mL | 0.1527 mL | 0.3054 mL | 0.7636 mL |
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