Referencia número: HY-124617

AMXT-1501

11 Cited Publications

Revisión del cliente

Based on 11 publication(s) in Google Scholar

AMXT-1501 is a Bacterial agent and polyamine transport system inhibitor. AMXT-1501 targets membrane phospholipids and exhibits antibacterial activity against a variety of Gram-positive and Gram-negative multidrug-resistant bacteria. AMXT-1501 inhibits capsular biosynthesis in Streptococcus pneumoniae. AMXT-1501 targets ornithine decarboxylase and polyamines to inhibit the proliferation of neuroblastoma cells. AMXT-1501 in combination with DFMO (HY-B0744) induces Apoptosis in neuroblastoma cells. AMXT-1501 is applicable to research related to multidrug-resistant bacterial infections, pneumococcal infections, Streptococcus pneumoniae infections, and neuroblastoma.

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Pureza: 99.44%
No. CAS: 441022-64-6
Fòrmula: C₃₂H₆₈N₆O₂
Peso molecular:568.92

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

AMXT-1501 Chemical Structure

No. CAS: 441022-64-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Ensayo clínico
Chemical Information
Publications (11)
Solvente y solubilidad
- In Vitro
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target

Polyamine transport^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
MDA-MB-231	IC₅₀	57 μM Compound: 11, D-Lys(C(16)acyl)-Spm	Cytotoxicity against human MDA-MB-231 cells after 6 days by MTS/PMS dye assay Cytotoxicity against human MDA-MB-231 cells after 6 days by MTS/PMS dye assay	[PMID: 19281226]
MDA-MB-231	IC₅₀	62 μM Compound: 10	Cytotoxicity against human MDA-MB-231 cells after 6 days by MTS/PMS dye assay Cytotoxicity against human MDA-MB-231 cells after 6 days by MTS/PMS dye assay	[PMID: 19281226]

In Vitro

AMXT-1501 (1.56-50 μM) exhibits dose-dependent antibacterial activity against a variety of Gram-positive and Gram-negative multidrug-resistant (MDR) bacterial isolates, with MIC₅₀/MIC₉₀ values ranging from 3.13 to 12.5 μM^[1].
AMXT-1501 (8× MIC; 0-24 h) exhibits rapid and potent bactericidal activity against planktonic MRSA, ESBL-producing E. coli, and CR E. coli^[1].
AMXT-1501 (1-4× MIC; 0.5-24 h) reduces biofilm formation in a dose-dependent manner in most tested Staphylococcus aureus (MSSA and MRSA) and Enterococcus faecalis strains, causes severe damage to the cell membranes of MRSA and CR E. coli, increases membrane permeability and depolarizes them, thereby leading to bacterial cell death^[1].
AMXT-1501 (3.125-50 μg/mL; 90 s) binds directly to the bacterial membrane phospholipids CL and PG^[1].
AMXT-1501 (7.4 μM; cultured until OD₆₀₀ reaches 0.2-0.5) inhibits capsular polysaccharide biosynthesis by 61% in Streptococcus pneumoniae serotype 2 (strain D39), depletes intracellular polyamines and glucuronic acid, and simultaneously increases intracellular glucose levels^[2].
AMXT-1501 (7.4 μM) regulates gene expression in Streptococcus pneumoniae D39, thereby enhancing the biosynthesis of polyamines and glucose, inhibiting ATP production and fatty acid synthesis, and upregulating stress response pathways^[3].
AMXT-1501 (0.39-50 μM; 48 h) potently inhibits the viability of human neuroblastoma cell lines BE (2)-C, SMS-KCNR and SH-SY5Y in vitro, with IC₅₀ values of 17.69 μM, 17.72 μM and 14.13 μM, respectively^[4].
AMXT-1501 (2.5 μM; 96 h) induced apoptosis in BE (2)-C, SMS-KCNR and SH-SY5Y human neuroblastoma cells when used in combination with DFMO^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[4]

Cell Line:

BE(2)-C, SMS-KCNR, SH-SY5Y
Concentration:

0.39-50 μM
Incubation Time:

48 h
Result:

Induced cytotoxicity with IC₅₀ values of 17.69 μM (BE(2)-C), 17.72 μM (SMS-KCNR), and 14.13 μM (SH-SY5Y).

Apoptosis Analysis^[4]

Cell Line:

BE(2)-C, SMS-KCNR, SH-SY5Y
Concentration:

2.5 μM+2.5 mM DFMO
Incubation Time:

96 h
Result:

Induced apoptosis.
Reduced the amount of uncleaved PARP and increased the amounts of cleaved PARP and cleaved caspase-3.

In Vivo

AMXT-1501 (20 mg/kg; i.p.; every 12 h; 3 days) significantly reduces the size of skin abscesses and decreases MRSA loads in BALB/c mice^[1].
AMXT-1501 (20 mg/kg; i.p.; every 12 h; 3 days) significantly improves the survival rate of BALB/c mice with bacterial abdominal infection and reduces the bacterial load of CRE E. coli in their lung and liver tissues^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c (female, 6-8-week-old, 18-22 g) (S. aureus YUSA145 (MRSA)(1×107 CFU))^[1]
Dosage:

20 mg/kg
Administration:

i.p.; every 12 h; 3 days
Result:

Reduced skin abscess areas significantly.
Lowered bacterial loads in abscess tissue (log₁₀ CFU/g) significantly.

Animal Model:

BALB/c (female, 6-8-week-old, 18-22 g) (E. coli ECO2219 (CRE)(2×10⁹ CFU))^[1]
Dosage:

20 mg/kg
Administration:

i.p.; every 12 h; 3 days
Result:

Achieved a 50% survival rate through day 7, which was higher than the survival rate in tigecycline-treated mice.
Lowered bacterial loads in lung tissue (log₁₀ CFU/g) significantly.
Lowered bacterial loads in liver tissue (log₁₀ CFU/g) significantly.

Ensayo clínico

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

No. CAS 441022-64-6
Appearance Solid
Peso molecular 568.92
Fòrmula C₃₂H₆₈N₆O₂
Color White to off-white
SMILES

CCCCCCCCCCCCCCCC(NCCCC[C@@H](N)C(NCCCNCCCCNCCCN)=O)=O
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (11)

All Cell Proliferation/Viability AssayCell Imaging/StainingWB2D/3D Cell Culture and Differentiation

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Journal Impact Factor

Immunity

Polyamines regulate adaptive antitumor immunity by functional specialization of regulatory T cells. [Abstract]

2025 Aug 12;58(8):2019-2034.e11. PMID: 40749666
Nat Immunol

Epigenetic reprogramming of T cell metabolism restores function and enhances anti-tumor immunity in lung cancer. [Abstract]

2026 Jun;27(6):1268-1281. PMID: 42120791
Nat Commun

Polyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer. [Abstract]

2024 Mar 19;15(1):2461. PMID: 38504107
Cell Death Differ

Anaplastic Lymphoma Kinase signaling stabilizes SLC3A2 expression via MARCH11 to promote neuroblastoma cell growth. [Abstract]

2024 Jul;31(7):910-923. PMID: 38858548

AMXT-1501 purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2024 Jul;31(7):910-923. [Abstract]

IC50s of AMXT-1501 (4, 8, 12, 16, 20, 24 μM) in CLB-BAR, CLB-GE, SK-N-BE(2) and SH-SY5Y cells after 5 d treatment.

AMXT-1501 purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2024 Jul;31(7):910-923. [Abstract]

Immunoblot analysis of neuronal differentiation markers (RET and DLG2) in SK-N-BE(2) and SH-SY5Y cells treated with AMXT-1501 (0 or 8 µM) and RA (0 or 5 µM) for 48 h or 24 h .

AMXT-1501 purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2024 Jul;31(7):910-923. [Abstract]

Cells after AMXT-1501 (0 or 8 µM) and RA (0 or 5 µM) treatment as indicated for 48 h or 24 h.
Neuro Oncol

Polyamine acetylation mediates crosstalk between cancer cells and myeloid cells to promote mesenchymal/plurimetabolic states in glioblastoma. [Abstract]

2025 May 27:noaf128. PMID: 40424600
Emerg Microbes Infect

AMXT-1501 targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria. [Abstract]

2024 Dec;13(1):2321981. PMID: 38422452
Icahn School of Medicine at Mount Sinai

2025.
The University of Texas at San Antonio

2025.
bioRxiv

2025 January 26.
bioRxiv

Genetically encoded fluorescent reporter for polyamines. [Abstract]

2024 Nov 17:2024.08.24.609500. PMID: 39253442

AMXT-1501 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Nov 17:2024.08.24.609500. [Abstract]

Representative fluorescence images of cells upon indicated treatments. AMXT-1501 (0.5 μM; polyamine import inhibitor), DFMO (1 mM), and spermidine (5 μM).
Research Square Preprint

2024 Mar 19.

Solvente y solubilidad

In Vitro:

H₂O : 100 mg/mL (175.77 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7577 mL

8.7886 mL

17.5772 mL

5 mM

0.3515 mL

1.7577 mL

3.5154 mL

10 mM

0.1758 mL

0.8789 mL

1.7577 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (274 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Zheng J, et al. AMXT-1501 targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria. Emerg Microbes Infect. 2024;13(1):2321981. [Content Brief]

[2]. Ayoola MB, et al. Difluoromethylornithine (DFMO) and AMXT 1501 inhibit capsule biosynthesis in pneumococci. Sci Rep. 2022;12(1):11804. Published 2022 Jul 12. [Content Brief]

[3]. Ayoola MB, Shack LA, Phanstiel O 4th, Nanduri B. Impact of Difluoromethylornithine and AMXT 1501 on Gene Expression and Capsule Regulation in Streptococcus pneumoniae. Biomolecules. 2024 Feb 2;14(2):178. [Content Brief]

[4]. Samal K, Zhao P, Kendzicky A, Yco LP, McClung H, Gerner E, Burns M, Bachmann AS, Sholler G. AMXT-1501, a novel polyamine transport inhibitor, synergizes with DFMO in inhibiting neuroblastoma cell proliferation by targeting both ornithine decarboxylase and polyamine transport. Int J Cancer. 2013 Sep 15;133(6):1323-33. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	1.7577 mL	8.7886 mL	17.5772 mL	43.9429 mL
	5 mM	0.3515 mL	1.7577 mL	3.5154 mL	8.7886 mL
	10 mM	0.1758 mL	0.8789 mL	1.7577 mL	4.3943 mL
	15 mM	0.1172 mL	0.5859 mL	1.1718 mL	2.9295 mL
	20 mM	0.0879 mL	0.4394 mL	0.8789 mL	2.1971 mL
	25 mM	0.0703 mL	0.3515 mL	0.7031 mL	1.7577 mL
	30 mM	0.0586 mL	0.2930 mL	0.5859 mL	1.4648 mL
	40 mM	0.0439 mL	0.2197 mL	0.4394 mL	1.0986 mL
	50 mM	0.0352 mL	0.1758 mL	0.3515 mL	0.8789 mL
	60 mM	0.0293 mL	0.1465 mL	0.2930 mL	0.7324 mL
	80 mM	0.0220 mL	0.1099 mL	0.2197 mL	0.5493 mL
	100 mM	0.0176 mL	0.0879 mL	0.1758 mL	0.4394 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

AMXT-1501

11 Cited Publications

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AMXT-1501

Anti-Infection Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Clinical Compound Library

Drug Repurposing Compound Library

Antibacterial Compound Library

Orally Active Compound Library

Anti-Cancer Metabolism Compound Library

Cell Death Library

Anti-Drug-Resistant Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

Clinical Compound Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) AMXT-1501

Actividad biológica

Chemical Information

Publications (11)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

AMXT-1501

Anti-Infection Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Clinical Compound Library

Drug Repurposing Compound Library

Antibacterial Compound Library

Orally Active Compound Library

Anti-Cancer Metabolism Compound Library

Cell Death Library

Anti-Drug-Resistant Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

Clinical Compound Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) AMXT-1501

Actividad biológica

Chemical Information

Publications (11)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

AMXT-1501 Related Classifications

HY-124617 Related Classifications