C14-4
Based on 1 Customer Validation
C14-4 is an ionizable lipid utilized for the synthesis of lipid nanoparticles (LNPs). C14-4 enhances mRNA delivery, enabling the effective transport of mRNA to primary human T cells, which in turn induces functional protein expression. C14-4 demonstrates high transfection efficiency while maintaining low cytotoxicity.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.26%
- No. CAS: 2639634-80-1
- Fòrmula: C84H173N5O7
- Peso molecular:1365.30
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Almacenamiento:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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Metabolism/Protease Compound Library
HY-L012
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Lipid Compound Library
HY-L043
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Liposome Library
HY-L214
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Ionizable Lipid Compound Library
HY-L251
Actividad biológica
C14-4 (30 ng C14-4 LNPs/60000 cells, 48 h) promotes the expression of luciferase in primary human T cell Jurkat cells by enhancing mRNA delivery with low cytotoxicity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Jurkat cells
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Concentration:30 ng C14-4 LNPs/60000 cells
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Incubation Time:48 h
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Result:Promoted the expression of luciferase and had minimal cytotoxicity.
Chemical Information
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No. CAS 2639634-80-1
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Appearance Liquid (Density: 0.946±0.06 g/cm3)
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Peso molecular 1365.30
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Fòrmula C84H173N5O7
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Color Colorless to light yellow
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SMILES
OC(CCCCCCCCCCCC)CN(CCN1CCN(CC1)CCOCCN(CC(CCCCCCCCCCCC)O)CC(CCCCCCCCCCCC)O)CCOCCN(CC(CCCCCCCCCCCC)O)CC(CCCCCCCCCCCC)O
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Synonyms
C14-494
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
Ethanol : 100 mg/mL (73.24 mM; Need ultrasonic)
DMSO : 100 mg/mL (73.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (269 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 0.7324 mL | 3.6622 mL | 7.3244 mL | 18.3110 mL |
| 5 mM | 0.1465 mL | 0.7324 mL | 1.4649 mL | 3.6622 mL | |
| 10 mM | 0.0732 mL | 0.3662 mL | 0.7324 mL | 1.8311 mL | |
| 15 mM | 0.0488 mL | 0.2441 mL | 0.4883 mL | 1.2207 mL | |
| 20 mM | 0.0366 mL | 0.1831 mL | 0.3662 mL | 0.9155 mL | |
| 25 mM | 0.0293 mL | 0.1465 mL | 0.2930 mL | 0.7324 mL | |
| 30 mM | 0.0244 mL | 0.1221 mL | 0.2441 mL | 0.6104 mL | |
| 40 mM | 0.0183 mL | 0.0916 mL | 0.1831 mL | 0.4578 mL | |
| 50 mM | 0.0146 mL | 0.0732 mL | 0.1465 mL | 0.3662 mL | |
| 60 mM | 0.0122 mL | 0.0610 mL | 0.1221 mL | 0.3052 mL |
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