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Referencia número: HY-P10812

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CP61

CP61 is a CtBP1 and CtBP2 inhibitor that binds to dimeric CtBP1 and allosterically inhibits NADH (HY-113355) binding, disrupting the NADH-dependent dimerization of CtBP1 and CtBP2, inducing aberrant mitosis and micronucleus formation, thereby inhibiting cell proliferation and colony formation. CP61 can be used in breast cancer research.

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No. CAS: 1631984-43-4
Fòrmula: C₅₀H₇₃N₁₃O₁₂S
Peso molecular:1080.26

Almacenamiento:

Please store the product under the recommended conditions in the Certificate of Analysis.

CP61 Chemical Structure

No. CAS: 1631984-43-4

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- In Vitro
Chemical Information
Pureza y Documentación
Referencias
Calculators
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Actividad biológica

Descripciòn

IC₅₀ & Target

^[1]

CtBP1

CtBP2

In Vitro

Anti-cancerCell Cycle RegulationCarbohydrate MetabolismOthers

Anti-cancer

Cell Cycle Regulation

CP61 introduces a cysteine by mutating its own SGW sequence to CGW and is conjugated via a disulfide bond to the N-terminally Cys-modified HIV TAT cell-penetrating peptide (GRKKRRQRRRPPQ), thereby achieving intracellular delivery of the cyclic peptide. The resulting CP61-TAT retains CFP-CtBP1 in the cytoplasm of COS-7 cells without altering total CtBP1/CtBP2 protein levels; it dose-dependently increases the proportion of micronuclei in MCF-7 cells, raises the fraction of abnormal mitosis and prolongs mitotic duration, and inhibits MCF-7 cell proliferation, and this effect is reproducible in ER-negative highly metastatic MDA-MB-231 cells^[1].

Carbohydrate Metabolism

Cell Cycle Regulation

Anti-cancer

CP61 (50 μM; microinjection) selectively increases abnormal mitosis and prolongs mitotic duration in highly glycolytic MCF-7 cells, but has no such effect in MDA-MB-453 cells with lower glycolysis^[1].

Others

CP61 inhibits CtBP2 homodimerization in ELISA assays, IC₅₀ = 19 μM^[1].
CP61 dose-dependently disrupts the GST-CtBP1/His-CtBP1 complex in the presence of 10 μM NADH^[1].
CP61 allosterically and dose-dependently reduces the CtBP1-NADH FRET signal (Trp 285 nm, NADH 425 nm), and the maximal FRET is suppressed under saturating NADH, suggesting that it does not occupy the NADH pocket^[1].
CP61 shows no inhibitory effect on other Rossmann fold-containing NAD⁺/NADH-dependent dehydrogenases at concentrations up to 100 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

No. CAS 1631984-43-4
Peso molecular 1080.26
Fòrmula C₅₀H₇₃N₁₃O₁₂S
Sequence

cyclo(Ser-Gly-Trp-Thr-Val-Val-Arg-Met-Tyr)
Sequence Shortening

cyclo(SGWTVVRMY)
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (293 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Birts CN, et al. A cyclic peptide inhibitor of C-terminal binding protein dimerization links metabolism with mitotic fidelity in breast cancer cells. Chemical science. 2013 Aug 01;4(8):3046-3057. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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