DOTAP mesylate
Based on 23 publication(s) in Google Scholar
DOTAP (1,2-Dioleoyl-3-trimethylammonium-propane) mesylate is a cationic lipid reagent, a cationic derivative of trimethylammonium, linked to two 18-carbon fatty acid tails, each with a single olefin group. DOTAP methylsulfat can self-assemble with negatively charged ions (such as DNA) to form complexes, which can be adsorbed to the cell membrane surface and enter the cell by electrostatic interaction and endocytosis, respectively. DOTAP mesylate promotes endosomal membrane fusion with its own hydrophobic domain, releases DNA into the cytoplasm, and exerts gene delivery function. DOTAP mesylate can be widely used in research fields such as gene therapy, cell transfection, and non-viral vector design
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- Pureza : 99.95%
- No. CAS: 252769-92-9
- Fòrmula: C43H83NO7S
- Peso molecular:758.19
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Almacenamiento:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DOTAP mesylate
More- Signal Transduct Target Ther. 2025 Mar 24;10(1):101. [Abstract]
- Immunity. 2026 Feb 10;59(2):270-287.e11. [Abstract]
- Exploration. 2024 Jun 26;5(1):20240002. [Abstract]
- Nat Immunol. 2025 Oct;26(10):1660-1672. [Abstract]
- J Adv Res. 2026 May 4:S2090-1232(26)00378-4. [Abstract]
- J Nanobiotechnology. 2024 Nov 27;22(1):733. [Abstract]
- Adv Sci (Weinh). 2026 Sep 16:e77817.
- Cell Rep Med. 2026 Mar 17;7(3):102667. [Abstract]
- Carbohydr Polym. 2026 Jul 1:383:125313. [Abstract]
- Asian J Pharm Sci. 2026 Jan 10.
- J Control Release. 2026 May 23:396:115047. [Abstract]
- Adv Healthc Mater. 2023 Jan;12(6):e2202412. [Abstract]
- Biofabrication. 2026 Aug 17;18(3):035046.
- Pharmaceutics. 2023 Apr 30;15(5):1379. [Abstract]
- Mikrochim Acta. 2026 Jul 1;193(7):501.
- Nanoscale. 2023 Nov 16;15(44):17825-17838. [Abstract]
- PLoS Pathog. 2026 Mar 17;22(3):e1014062. [Abstract]
- Microbiol Spectr. 2025 Nov 5;13(12):e0143825. [Abstract]
- bioRxiv. 2026 May 4.
- bioRxiv. 2026 Apr 27.
- bioRxiv. 2025 Oct 1:2025.10.01.679765. [Abstract]
- University of Washington. 2025.
- SSRN. 2024 Nov 12.
Customer Validation & Images
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Gel Electrophoresis
Actividad biológica
Descripciòn
In Vitro
Different mass ratios of DOTAP mesylate (different mass ratios; 4 hours transfection + 24 hours culture) and DOPE (HY-112005) have different optimal transfection efficiencies. In Huh7 and AGS cells, the optimal ratio is T1P0 (1:0)/T3P1 (3:1), and in A549 cells, the optimal ratio is T1P1 (1:1)/T3P1 (1:3), which is independent of liposome size or zeta potential[1].
DOTAP mesylate mixed with lipids in certain ratios (e.g., 1:2, 1:1, 2:1) is more efficient than DOTAP mesylate alone in transfecting DNA, and still maintains transfection activity at 80% serum concentration. And the optimal transfection ratio of DOTAP mesylate to DNA is 12:1[2].
DOTAP mesylate (30 min DNase treatment) DNA-lipid complexes remain stable to DNase I digestion in the presence of serum, and the addition of cholesterol does not affect the structural integrity of the complex[2].
DOTAP mesylate (containing 1% NBD-DOTAP mesylate label; 2 h transfection) in flow cytometric analysis, cholesterol-modified liposomes bind to cells at a significantly higher rate in serum than pure DOTAP methylsulfat liposomes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 252769-92-9
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Appearance Solid
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Peso molecular 758.19
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Fòrmula C43H83NO7S
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Color White to off-white
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SMILES
S(C)(=O)(=O)[O-].C(OC(CCCCCCC/C=C\CCCCCCCC)=O)(COC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C
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Synonyms
1,2-Dioleoyl-3-trimethylammonium-propane mesylate
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (23)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Intranasal prime-boost RNA vaccination elicits potent T cell response for lung cancer therapy. [Abstract]2025 Mar 24;10(1):101. PMID: 40122855 -
Immunity
Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells. [Abstract]2026 Feb 10;59(2):270-287.e11. PMID: 41672043 -
Exploration
A pH responsive nanocomposite for combination sonodynamic-immunotherapy with ferroptosis and calcium ion overload via SLC7A11/ACSL4/LPCAT3 pathway. [Abstract]2024 Jun 26;5(1):20240002. PMID: 40040833 -
Nat Immunol
Delaying pyroptosis with an AI-screened gasdermin D pore blocker mitigates inflammatory response. [Abstract]2025 Oct;26(10):1660-1672. PMID: 40954252 -
J Adv Res
4-Octyl itaconate-induced Nrf2 nuclear translocation mitigates Caspase-11-dependent noncanonical macrophage pyroptosis and acute lung injury. [Abstract]2026 May 4:S2090-1232(26)00378-4. PMID: 42092588 -
J Nanobiotechnology
Sele-targeted siRNA liposome nanoparticles inhibit pathological scars formation via blocking the cross-talk between monocyte and endothelial cells: a preclinical study based on a novel mice scar model. [Abstract]2024 Nov 27;22(1):733. PMID: 39593088 -
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Cell Rep Med
Engineering lipid nanoparticle-stabilized emulsions for spatiotemporal mRNA delivery and enhanced T cell immunity. [Abstract]2026 Mar 17;7(3):102667. PMID: 41850240 -
Carbohydr Polym
Hyaluronic acid-engineered infection-responsive liposomes achieve biofilm penetration and synergistic photothermal-photodynamic antibacterial therapy. [Abstract]2026 Jul 1:383:125313. PMID: 42067348 -
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J Control Release
Antimicrobial peptide-derived lipid nanoparticles enable lung-targeted mRNA therapy for multidrug-resistant bacterial pneumonia. [Abstract]2026 May 23:396:115047. PMID: 42178062 -
Adv Healthc Mater
2023 Jan;12(6):e2202412. PMID: 36412002 -
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Pharmaceutics
MiR-126-Loaded Immunoliposomes against Vascular Endothelial Inflammation In Vitro and Vivo Evaluation. [Abstract]2023 Apr 30;15(5):1379. PMID: 37242620
DOTAP mesylate purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2023 Apr 30;15(5):1379. [Abstract]
(A) Identification of miR-126 conjugates using gel retardation assay. 1, miR-126; 2, Va-CLs; 3–8, DOTAP (DOTAP chloride):miR-126 = 16:1, 24:1, 32:1, 40:1, 48:1 and 56:1 incubated for 30 min at room temperature to obtain Va-CLs/miR-126 and CLs/miR-126 complexes. (B) The gray value of each miR-126 band was calculated
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Nanoscale
Differential cellular responses to FDA-approved nanomedicines: an exploration of albumin-based nanocarriers and liposomes in protein corona formation. [Abstract]2023 Nov 16;15(44):17825-17838. PMID: 37850423 -
PLoS Pathog
LL-37 selectively targets Plasmodium-infected erythrocytes and exhibits antimalarial activity. [Abstract]2026 Mar 17;22(3):e1014062. PMID: 41843625 -
Microbiol Spectr
Lipid nanoparticle-delivered mRNA vaccine encoding the MOMP of Chlamydia psittaci elicits protective immune responses in BALB/c mice. [Abstract]2025 Nov 5;13(12):e0143825. PMID: 41190885 -
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bioRxiv
Genus-specific remodeling of carbon and energy metabolism facilitates acetoclastic methanogenesis in Methanosarcina spp. and Methanothrix spp. [Abstract]2025 Oct 1:2025.10.01.679765. PMID: 41256488 -
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Solvente y solubilidad
In Vitro:
Ethanol : 33.33 mg/mL (43.96 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (2.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: 2 mg/mL (2.64 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2 mg/mL (2.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Pureza y Documentación
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Ficha de datos (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Kim BK, et al. DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes. Biochim Biophys Acta. 2015 Oct;1848 (10 Pt A) :1996-2001. [Content Brief]
[2]. Crook K, et al. Inclusion of cholesterol in DOTAP transfection complexes increases the delivery of DNA to cells in vitro in the presence of serum. Gene Ther. 1998 Jan;5 (1) :137-43. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 1.3189 mL | 6.5947 mL | 13.1893 mL | 32.9733 mL |
| 5 mM | 0.2638 mL | 1.3189 mL | 2.6379 mL | 6.5947 mL | |
| 10 mM | 0.1319 mL | 0.6595 mL | 1.3189 mL | 3.2973 mL | |
| 15 mM | 0.0879 mL | 0.4396 mL | 0.8793 mL | 2.1982 mL | |
| 20 mM | 0.0659 mL | 0.3297 mL | 0.6595 mL | 1.6487 mL | |
| 25 mM | 0.0528 mL | 0.2638 mL | 0.5276 mL | 1.3189 mL | |
| 30 mM | 0.0440 mL | 0.2198 mL | 0.4396 mL | 1.0991 mL | |
| 40 mM | 0.0330 mL | 0.1649 mL | 0.3297 mL | 0.8243 mL |
DOTAP mesylate Related Classifications
HY-W127426 Related Classifications
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