Enterobactin
Based on 1 Customer Validation
Enterobactin is a siderophore produced by Gram-negative bacteria and has an extremely high affinity for iron. During the process of Salmonella typhimurium infecting macrophages, Enterobactin can not only help bacteria uptake iron but also reduce the antibacterial activity of macrophages. In addition, Enterobactin is also involved in the oxidative stress response of Escherichia coli. Enterobactin can be hydrolyzed by Fes in the cell and exert antioxidant effects by scavenging free radicals.
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- Pureza: 98.02%
- No. CAS: 28384-96-5
- Fòrmula: C30H27N3O15
- Peso molecular:669.55
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Actividad biológica
Enterobactin (25 μM; 1-24 h) inhibits the intracellular antibacterial response of macrophages to Salmonella typhimurium infection and disrupts macrophage iron homeostasis and M1/M2 polarization[1].
Enterobactin (1-50 μM) can be hydrolyzed by Fes in the cytoplasm of Escherichia coli, releasing free hydroxyl groups and exerting antioxidant effects by scavenging free radicals[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 28384-96-5
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Appearance Solid
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Peso molecular 669.55
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Fòrmula C30H27N3O15
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Color White to off-white
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SMILES
O=C1OC[C@H](NC(C2=CC=CC(O)=C2O)=O)C(OC[C@H](NC(C3=CC=CC(O)=C3O)=O)C(OC[C@@H]1NC(C4=CC=CC(O)=C4O)=O)=O)=O
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Structure Classification
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Initial Source
Salmonella typhimurium
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (149.35 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.73 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.73 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Saha P, et al. The bacterial siderophore enterobactin confers survival advantage to Salmonella in macrophages. Gut Microbes. 2019;10(3):412-423. [Content Brief]
[2]. Peralta DR, et al. Enterobactin as Part of the Oxidative Stress Response Repertoire. PLoS One. 2016 Jun 16;11(6):e0157799. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4935 mL | 7.4677 mL | 14.9354 mL | 37.3385 mL |
| 5 mM | 0.2987 mL | 1.4935 mL | 2.9871 mL | 7.4677 mL | |
| 10 mM | 0.1494 mL | 0.7468 mL | 1.4935 mL | 3.7339 mL | |
| 15 mM | 0.0996 mL | 0.4978 mL | 0.9957 mL | 2.4892 mL | |
| 20 mM | 0.0747 mL | 0.3734 mL | 0.7468 mL | 1.8669 mL | |
| 25 mM | 0.0597 mL | 0.2987 mL | 0.5974 mL | 1.4935 mL | |
| 30 mM | 0.0498 mL | 0.2489 mL | 0.4978 mL | 1.2446 mL | |
| 40 mM | 0.0373 mL | 0.1867 mL | 0.3734 mL | 0.9335 mL | |
| 50 mM | 0.0299 mL | 0.1494 mL | 0.2987 mL | 0.7468 mL | |
| 60 mM | 0.0249 mL | 0.1245 mL | 0.2489 mL | 0.6223 mL | |
| 80 mM | 0.0187 mL | 0.0933 mL | 0.1867 mL | 0.4667 mL | |
| 100 mM | 0.0149 mL | 0.0747 mL | 0.1494 mL | 0.3734 mL |
Enterobactin Related Classifications
HY-128525 Related Classifications
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