Grape seed extract
Based on 2 publication(s) in Google Scholar
Grape seed extract is a natural product, with anti-inflammatory and anti-proliferative effects. Grape seed extract shows inhibitory activity on the fat-metabolizing enzymes pancreatic lipase and lipoprotein lipase. Grape seed extract induces apoptotic in human colorectal cancer cells.
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- Assay : 95.15%
- No. CAS: 84929-27-1
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Almacenamiento:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Grape seed extract
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Actividad biológica
Descripciòn
In Vitro
Grape seed extract (25-100 μg/mL; 12-48 hours) inhibits growth and induces death in human colorectal cancer cells[3].
Grape seed extract (25-100 μg/mL; 12-24 hours) modulates the protein levels of cell cycle regulatory molecules in HT29 and LoVo cells[3].
Grape seed extract (25-100 μg/mL; 24 hours) causes apoptotic cell death of human colorectal cancer cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT29 cells, LoVo cells
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Concentration:25 μg/mL, 50 μg/mL, 100 μg/mL
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Incubation Time:12 hours, 24 hours, 48 hours
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Result:Inhibited cell growth in a dose- and a time-dependent manner.
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Cell Line:HT29 cells, LoVo cells
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Concentration:25 μg/mL, 50 μg/mL, 100 μg/mL
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Incubation Time:12 hours, 24 hours, 48 hours
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Result:Induced cell cycle arrest (a marked increase in Cip1/p21protein level and a decrease in G 1 phase^associated cyclins and cyclin-dependentkinases).
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Cell Line:HT29 cells, LoVo cells
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Concentration:25 μg/mL, 50 μg/mL, 100 μg/mL
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Incubation Time:12 hours, 24 hours
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Result:Modulates the protein levels of CDKIs, CDKs, and cyclins.
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Cell Line:HT29 cells, LoVo cells
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Concentration:25 μg/mL, 50 μg/mL, 100 μg/mL
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Incubation Time:24 hours
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Result:Showed a significant dose-dependent increase in apoptotic cell population in HT29 cells at 50 μg/mL and 100 μg/mL; all three Concentrations caused a dose-dependent increase in apoptotic cells.
In Vivo
Grape seed extract inhibits cell proliferation but increases apoptotic cell death in tumors[3].
Grape seed extract enhances Cip1/p21protein levels and poly(ADP-ribose) poly-merase cleavage in tumors[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Athymic male nu/nu mice, with human colon carcinoma HT29 tumor xenograft[3]
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Dosage:200 mg/kg
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Administration:Oral gavage, 5 days/wk, for 8 weeks
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Result:Showed time-dependent inhibition of tumor growth.
Ensayo clínico
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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No. CAS 84929-27-1
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Appearance Solid
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Color Brown to reddish brown
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SMILES
[Grape seed extract]
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell Rep
2025 Apr 16;44(5):115572. PMID: 40249703 -
Int J Mol Sci
Stearoyl-CoA Desaturases1 Accelerates Non-Small Cell Lung Cancer Metastasis by Promoting Aromatase Expression to Improve Estrogen Synthesis. [Abstract]2023 Apr 6;24(7):6826. PMID: 37047797
Solvente y solubilidad
In Vitro:
DMSO : 6 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.6 mg/mL; Clear solution
This protocol yields a clear solution of ≥ 0.6 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.6 mg/mL; Clear solution
This protocol yields a clear solution of ≥ 0.6 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. The effects of grape seed extract (Vitis vinifera) supplement on inflammatory markers, neuropeptide Y, anthropometric measures, and appetite in obese or overweight individuals: A randomized clinical trial. [Content Brief]
[2]. Inhibitory effects of grape seed extract on lipases. Nutrition. 2003 Oct;19(10):876-9. [Content Brief]
[3]. Manjinder Kaur, et al. Grape seed extract inhibits in vitro and in vivo growth of human colorectal carcinoma cells. Clin Cancer Res. 2006 Oct 15;12(20 Pt 1):6194-202. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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