M1219
M1219 is a GSH/ATP dual near-infrared activated fluorescent probe that enables independent real-time monitoring of dynamic changes in intracellular GSH and ATP without spectral crosstalk (GSH: Ex=640 nm, Em=740~800 nm; ATP: Ex=594 nm/610 nm, Em=650~700 nm). M1219 not only visualizes the metabolic regulatory mechanism of TNBC under single/dual-target inhibition of SLC7A11/GLUT1 and accurately evaluates its in vivo efficacy, but also achieves precise localization of the TNBC tumor invasion boundary. M1219 can be used for the research of triple-negative breast cancer.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Fòrmula: C76H83ClN8O5S
- Peso molecular:1256.04
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
- Pureza y Documentación
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Help & FAQs
Actividad biológica
M1219 (0-24 h) is a bifunctional near-infrared fluorescent probe that can specifically, stably and independently detect GSH and ATP in cell-free buffer systems. Its detection range matches physiological concentrations, with an EC50 value of 6.711 mM for GSH and 4.108 mM for ATP[1].
M1219 (20 μM; 15 min) enables specific and independent detection of endogenous GSH and ATP in live 4T1 cells, thus allowing visualization of the dynamic changes of these molecules under the action of redox and energy metabolism regulators[1].
M1219 (15-60 min) enables high-contrast fluorescence imaging in breast cancer specimens for precise identification of tumor boundaries, thereby accurately distinguishing cancerous tissues from adjacent tissues[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
M1219 (Intratumoral injection) enables precise near-infrared fluorescence-guided resection of subcutaneous triple-negative breast cancer (TNBC) tumors in mice, achieving a negative surgical margin of <0.1 mm through high-contrast differentiation between tumor and normal tissues[1].
M1219 (1 mmol in 100 μL; intravenous injection; observation for 24 h after administration) exhibits low in vivo toxicity and favorable biosafety in healthy BALB/c mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (female)[1]
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Dosage:0.5 mmol in 200 μL
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Administration:tail vein injection; on days 0, 10, and 18 post-treatment initiation
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Result:Showed similar dual-channel (GSH at 815 nm, ATP at 650 nm) fluorescence intensities in tumor regions across all treatment groups on day 0.
Reached GSH channel fluorescence intensity 0.091-fold of the PBS control group, and ATP channel fluorescence intensity 0.090-fold of the control group in tumors by day 18.
Confirmed ex vivo GSH and ATP channel fluorescence intensities of 0.1067-fold and 0.093-fold of the control group, respectively.
Demonstrated a significant negative correlation between fluorescence signal intensity and tumor growth inhibition and therapeutic efficacy.
Chemical Information
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Peso molecular 1256.04
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Fòrmula C76H83ClN8O5S
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SMILES
CCN(C1=CC2=C(C3(C4=C(C5=C(CC4)C=C(C=C5)N(C)C)O2)C6=C(C(N3CCN7CCN(C(CC[N+]8=C(C(C)(C9=C8C=CC=C9)C)/C=C/C%10=C(/C(CCC%10)=C/C=C%11N(C%12=C(C/%11(C)C)C=CC=C%12)C)SC%13=CC=C(C=C%13)[N+]([O-])=O)=O)CC7)=O)C=CC=C6)C=C1)CC.[Cl-]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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