Referencia número: HY-149302

MC4033

1 Cited Publications

Revisión del cliente

Based on 1 publication(s) in Google Scholar

MC4033 shows IC₅₀s of 39.4 μM, 52.1 μM, 41 μM and 30.1 μM in HCT116, H1299, A549 and U937, respectively.
MC4033 (25, 50, 100, and 200 μM, 72 h) reduces the level of H4K16Ac in HT29 cells, suggesting its ability to inhibit KAT8 in cells.

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Pureza: 98.26%
No. CAS: 28532-21-0
Fòrmula: C₁₆H₁₃N₃O₃
Peso molecular:295.29

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

MC4033 Chemical Structure

No. CAS: 28532-21-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (1)
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

MC4033 shows IC₅₀s of 39.4 μM, 52.1 μM, 41 μM and 30.1 μM in HCT116, H1299, A549 and U937, respectively^[1].
MC4033 (25, 50, 100, and 200 μM, 72 h) reduces the level of H4K16Ac in HT29 cells, suggesting its ability to inhibit KAT8 in cells^[1].

IC₅₀ & Target

12.1μM (lysine acetyltransferase 8, KAT8)^[1]

Ver todos los productos específicos de isoformas Histone Acetyltransferase :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	41 μM Compound: 19; MC4033	Antiproliferative activity against human A549 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay	[PMID: 37155735]
HCT-116	IC₅₀	39.4 μM Compound: 19; MC4033	Antiproliferative activity against human HCT-116 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay	[PMID: 37155735]
HT-29	IC₅₀	>100 μM Compound: 19; MC4033	Antiproliferative activity against human HT-29 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay	[PMID: 37155735]
NCI-H1299	IC₅₀	52.1 μM Compound: 19; MC4033	Antiproliferative activity against human NCI-H1299 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H1299 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay	[PMID: 37155735]
U-937	IC₅₀	30.1 μM Compound: 19; MC4033	Antiproliferative activity against human U-937 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human U-937 cells assessed as cell proliferation inhibition incubated for 72 hrs by MTT assay	[PMID: 37155735]

In Vitro

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

HT29, HCT116, HeLa, H1299, A549, H460, MCF7, U937, and U251 cells
Concentration:

10, 25, 50, and 100 μM
Incubation Time:

72 h
Result:

Displayed dose-dependent antiproliferative effects in HCT116, H1299, A549, and U937 cell lines.
The inhibition rate of cell proliferation was 70% at 50 μM and >80% at 100 μM in U937 cells.

Cell Cycle Analysis^[1]

Cell Line:

HT29, HCT116, and HeLa cells
Concentration:

50 μM and 100 μM
Incubation Time:

72 h
Result:

Propidium iodide (PI) staining showed a slight increase in the percentage of cells with DNA hypodiploid peak, indicative of apoptosis.

RT-PCR^[1]

Cell Line:

HCT116 cells
Concentration:

100 μM
Incubation Time:

48h
Result:

Reduced the mRNA levels of oncogenes UCP2.

Immunofluorescence^[1]

Cell Line:

HT29 cells
Concentration:

50 μM
Incubation Time:

24 h
Result:

Reduced H4K16Ac signal intensity by 80%.

Western Blot Analysis^[1]

Cell Line:

HCT116 cells
Concentration:

0,10, 25, 50,100 μM
Incubation Time:

48 h
Result:

Showed that the altered ratio of LC3-II/-I and the regulation of p62 autophagy markers indicated the activation of autophagy in HCT116 cells.

Apoptosis Analysis^[1]

Cell Line:

HCT116 cells
Concentration:

100 μM or 10 μM (MC4033/CQ)
Incubation Time:

72 h
Result:

Showed that exposure of HCT116 cells to CQ increased the apoptotic effect of KAT8i.

Chemical Information

No. CAS 28532-21-0
Appearance Solid
Peso molecular 295.29
Fòrmula C₁₆H₁₃N₃O₃
Color Light yellow to yellow
SMILES

CC1=NN(C2=CC=C(C=C2)C(O)=O)C(/C1=C\C3=CC=CN3)=O
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

Adv Sci (Weinh)

Blocking Lysine Crotonylation and Aerobic Glycolysis as Targeting Strategy Against mpox Virus Replication. [Abstract]

2025 Oct 27:e09148. PMID: 41144710

Solvente y solubilidad

In Vitro:

DMSO : 25 mg/mL (84.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3865 mL

16.9325 mL

33.8650 mL

5 mM

0.6773 mL

3.3865 mL

6.7730 mL

10 mM

0.3387 mL

1.6933 mL

3.3865 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (278 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Fiorentino F, et al. First-in-Class Selective Inhibitors of the Lysine Acetyltransferase KAT8. J Med Chem. 2023 May 25;66(10):6591-6616 [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.3865 mL	16.9325 mL	33.8650 mL	84.6625 mL
	5 mM	0.6773 mL	3.3865 mL	6.7730 mL	16.9325 mL
	10 mM	0.3387 mL	1.6933 mL	3.3865 mL	8.4663 mL
	15 mM	0.2258 mL	1.1288 mL	2.2577 mL	5.6442 mL
	20 mM	0.1693 mL	0.8466 mL	1.6933 mL	4.2331 mL
	25 mM	0.1355 mL	0.6773 mL	1.3546 mL	3.3865 mL
	30 mM	0.1129 mL	0.5644 mL	1.1288 mL	2.8221 mL
	40 mM	0.0847 mL	0.4233 mL	0.8466 mL	2.1166 mL
	50 mM	0.0677 mL	0.3387 mL	0.6773 mL	1.6933 mL
	60 mM	0.0564 mL	0.2822 mL	0.5644 mL	1.4110 mL
	80 mM	0.0423 mL	0.2117 mL	0.4233 mL	1.0583 mL

Keywords

MC4033

1 Cited Publications

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MC4033

Apoptosis Compound Library

Epigenetics Compound Library

Histone Modification Research Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Covalent Screening Library

Cell Death Library

Bioactive Compound Library Max

Covalent Screening Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) MC4033

Ver todos los productos específicos de isoformas Histone Acetyltransferase

Actividad biológica

Chemical Information

Publications (1)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

MC4033

Apoptosis Compound Library

Epigenetics Compound Library

Histone Modification Research Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Covalent Screening Library

Cell Death Library

Bioactive Compound Library Max

Covalent Screening Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) MC4033

Ver todos los productos específicos de isoformas Histone Acetyltransferase

Actividad biológica

Chemical Information

Publications (1)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

MC4033 Related Classifications

HY-149302 Related Classifications