Mito-TP-2
Based on 1 Customer Validation
Mito-TP-2 is a triptolide (HY-32735) derivative. Mito-TP-2 exhibits concentration-dependent cytotoxicity in cancer cells. Mito-TP-2 is selectively driven and accumulated into the mitochondria of tumor cells by mitochondrial transmembrane potential and exerts specific mitochondrial toxicity. Mito-TP-2 can be used for the research of liver cancer, breast cancer, and non-small cell lung cancer.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 96.82%
- No. CAS: 2489184-70-3
- Fòrmula: C44H48BrO7P
- Peso molecular:799.73
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Anti-Breast Cancer Compound Library
HY-L074
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Anti-Lung Cancer Compound Library
HY-L075
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Anti-Liver Cancer Compound Library
HY-L101
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Anti-Cancer Compound Library
HY-L025
Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
10 μM
Compound: Mito-TP-2
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Cytotoxicity against human A549 cells assessed as inhibition of cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as inhibition of cell viability incubated for 48 hrs by MTT assay
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[PMID: 38169372] |
Mito-TP-2 (48 h) potently inhibits HepG-2 human hepatocellular carcinoma cells (IC50=0.067 μM) and MCF-7 human breast cancer cells (IC50=0.238 μM), while showing 16-fold reduced toxicity to L-02 human normal hepatocytes (IC50=0.334 μM) compared to Triptolide (HY-32735)[1].
Mito-TP-2 (48 h) potently inhibits NCI-H1975 cell proliferation with an IC50 of 0.14 μM, while showing minimal activity against BEAS-2B, MCF-7, A549, and NCI-H460 cells[2].
Mito-TP-2 accumulates to a higher level than Triptolide (HY-32735) in HepG2 cell mitochondria, shows enhanced antitumor selectivity, and exhibits reduced toxicity toward normal cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2489184-70-3
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Appearance Solid
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Peso molecular 799.73
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Fòrmula C44H48BrO7P
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Color White to off-white
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SMILES
C[C@@]12[C@@]34[C@@]5([C@@](C[C@@]1([H])C6=C(C(OC6)=O)CC2)([H])O5)[C@@H]([C@@]7([C@](O7)([H])[C@]3([H])O4)C(C)C)OC(CCCCC[P+](C8=CC=CC=C8)(C9=CC=CC=C9)C%10=CC=CC=C%10)=O.[Br-]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (125.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (6.25 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (6.25 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.2504 mL | 6.2521 mL | 12.5042 mL | 31.2606 mL |
| 5 mM | 0.2501 mL | 1.2504 mL | 2.5008 mL | 6.2521 mL | |
| 10 mM | 0.1250 mL | 0.6252 mL | 1.2504 mL | 3.1261 mL | |
| 15 mM | 0.0834 mL | 0.4168 mL | 0.8336 mL | 2.0840 mL | |
| 20 mM | 0.0625 mL | 0.3126 mL | 0.6252 mL | 1.5630 mL | |
| 25 mM | 0.0500 mL | 0.2501 mL | 0.5002 mL | 1.2504 mL | |
| 30 mM | 0.0417 mL | 0.2084 mL | 0.4168 mL | 1.0420 mL | |
| 40 mM | 0.0313 mL | 0.1563 mL | 0.3126 mL | 0.7815 mL | |
| 50 mM | 0.0250 mL | 0.1250 mL | 0.2501 mL | 0.6252 mL | |
| 60 mM | 0.0208 mL | 0.1042 mL | 0.2084 mL | 0.5210 mL | |
| 80 mM | 0.0156 mL | 0.0782 mL | 0.1563 mL | 0.3908 mL | |
| 100 mM | 0.0125 mL | 0.0625 mL | 0.1250 mL | 0.3126 mL |
Mito-TP-2 Related Classifications
HY-183716 Related Classifications
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