MSC-4381
Based on 1 Customer Validation
MSC-4381 (MCT4-IN-1), a chemical probe, is an orally active and selective monocarboxylate transporter 4 (MCT4/SLC16A3) inhibitor with an IC50 of 77 nM and a Ki of 11 nM. MSC-4381 targets to the cytosolic domain of MCT4. MSC-4381 results in lactate efflux inhibition and reduction of cellular viability in MCT4 high expressing cells. MSC-4381 has the potential for MCT4 transporter inhibition research. MSC-4381 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
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- Pureza : 98.89%
- No. CAS: 2445185-57-7
- Fòrmula: C26H20ClN3O6S
- Peso molecular:537.97
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
IC50 & Target
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MCT4 77 nM (IC50) |
MCT4 11 nM (Ki) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| MDA-MB-231 | IC50 |
1 nM
Compound: 18n
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Cytotoxicity against human MDA-MB-231 cells assessed as reduction in intracellular lactate accumulation preincubated for 30 mins followed by D(+)glucose and measured after 4 hrs by UHPLC-ESI-Q-Orbitrap-MS analysis
Cytotoxicity against human MDA-MB-231 cells assessed as reduction in intracellular lactate accumulation preincubated for 30 mins followed by D(+)glucose and measured after 4 hrs by UHPLC-ESI-Q-Orbitrap-MS analysis
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[PMID: 34382802] |
| MDA-MB-231 | IC50 |
11 nM
Compound: 18n
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Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 6 days by MTS assay
Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 6 days by MTS assay
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[PMID: 34382802] |
| MIA PaCa-2 | IC50 |
4720 nM
Compound: 18n
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Cytotoxicity against human MIA PaCa-2 cells assessed as inhibition of cell growth incubated for 6 days by MTS assay
Cytotoxicity against human MIA PaCa-2 cells assessed as inhibition of cell growth incubated for 6 days by MTS assay
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[PMID: 34382802] |
| MIA PaCa-2 | IC50 |
>25000 nM
Compound: 18n
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Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in intracellular lactate accumulation preincubated for 30 mins followed by D(+)glucose and measured after 4 hrs by UHPLC-ESI-Q-Orbitrap-MS analysis
Cytotoxicity against human MIA PaCa-2 cells assessed as reduction in intracellular lactate accumulation preincubated for 30 mins followed by D(+)glucose and measured after 4 hrs by UHPLC-ESI-Q-Orbitrap-MS analysis
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[PMID: 34382802] |
| NCI-H358 | IC50 |
3 nM
Compound: 18n
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Cytotoxicity against human NCI-H358 cells assessed as reduction in intracellular lactate accumulation preincubated for 30 mins followed by D(+)glucose and measured after 4 hrs by UHPLC-ESI-Q-Orbitrap-MS analysis
Cytotoxicity against human NCI-H358 cells assessed as reduction in intracellular lactate accumulation preincubated for 30 mins followed by D(+)glucose and measured after 4 hrs by UHPLC-ESI-Q-Orbitrap-MS analysis
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[PMID: 34382802] |
| RT-4 | IC50 |
638 nM
Compound: 18n
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Cytotoxicity against human RT-4 cells assessed as reduction in intracellular lactate accumulation preincubated for 30 mins followed by D(+)glucose and measured after 4 hrs by UHPLC-ESI-Q-Orbitrap-MS analysis
Cytotoxicity against human RT-4 cells assessed as reduction in intracellular lactate accumulation preincubated for 30 mins followed by D(+)glucose and measured after 4 hrs by UHPLC-ESI-Q-Orbitrap-MS analysis
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[PMID: 34382802] |
| SNU-398 | IC50 |
>25000 nM
Compound: 18n
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Cytotoxicity against human SNU-398 cells assessed as reduction in intracellular lactate accumulation preincubated for 30 mins followed by D(+)glucose and measured after 4 hrs by UHPLC-ESI-Q-Orbitrap-MS analysis
Cytotoxicity against human SNU-398 cells assessed as reduction in intracellular lactate accumulation preincubated for 30 mins followed by D(+)glucose and measured after 4 hrs by UHPLC-ESI-Q-Orbitrap-MS analysis
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[PMID: 34382802] |
In Vitro
MSC-4381 (compound 18n) inhibits lactate efflux in the MDA-MB-231 cell line with an IC50 of 1 nM. The on-target activity is confirmed with a Ki of 11 nM by fluorescence cross-correlation spectroscopy (FCCS)[1].
MSC-4381 does not inhibit lactate efflux to a similar extent in SNU-398 and MiaPaca2, and only 600-fold less in RT-4 cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MSC-4381 (30 mg/kg/day; for 15 days) shows no significant antitumor activity[1].
MSC-4381 (0.2 mg/kg; iv) has a T1/2 of 1 hours, a CL of 0.33 L/h kg, a Cmax of 489 ng/mL and a Vss of 0.4 L/kg for mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:MC38 tumor-bearing C57/BL6 mice[1]
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Dosage:30 mg/kg
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Administration:PO; single dose
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Result:Only combined with MCT1/2 inhibitor exhibited a significant tumoral intracellular lactate accumulation.
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Animal Model:Mice[1]
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Dosage:0.2 mg/kg (Pharmacokinetic Analysis)
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Administration:IV
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Result:Had a T1/2 of 1 hours, a CL of 0.33 L/h•kg, a Cmax of 489 ng/mL and a Vss of 0.4 L/kg for mice.
Chemical Information
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No. CAS 2445185-57-7
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Appearance Solid
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Peso molecular 537.97
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Fòrmula C26H20ClN3O6S
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Color White to yellow
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SMILES
O=C(C1=NC=C(C#CC2=CC(Cl)=CC=C2NS(=O)(C3=C4N=CC=CC4=C(OCC)C=C3)=O)C(OC)=C1)O
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Synonyms
MCT4-IN-1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 83.33 mg/mL (154.90 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8588 mL | 9.2942 mL | 18.5884 mL | 46.4710 mL |
| 5 mM | 0.3718 mL | 1.8588 mL | 3.7177 mL | 9.2942 mL | |
| 10 mM | 0.1859 mL | 0.9294 mL | 1.8588 mL | 4.6471 mL | |
| 15 mM | 0.1239 mL | 0.6196 mL | 1.2392 mL | 3.0981 mL | |
| 20 mM | 0.0929 mL | 0.4647 mL | 0.9294 mL | 2.3235 mL | |
| 25 mM | 0.0744 mL | 0.3718 mL | 0.7435 mL | 1.8588 mL | |
| 30 mM | 0.0620 mL | 0.3098 mL | 0.6196 mL | 1.5490 mL | |
| 40 mM | 0.0465 mL | 0.2324 mL | 0.4647 mL | 1.1618 mL | |
| 50 mM | 0.0372 mL | 0.1859 mL | 0.3718 mL | 0.9294 mL | |
| 60 mM | 0.0310 mL | 0.1549 mL | 0.3098 mL | 0.7745 mL | |
| 80 mM | 0.0232 mL | 0.1162 mL | 0.2324 mL | 0.5809 mL | |
| 100 mM | 0.0186 mL | 0.0929 mL | 0.1859 mL | 0.4647 mL |
MSC-4381 Related Classifications
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