NIR‐fluorescent ceramide
NIR-fluorescent ceramide is a neutral near-infrared (NIF) fluorescent probe (λem=650 nm) based on the C-bridged nitrobenzoxadiazole SCOTfluor core, which enables visualization of lipid metabolic processes such as sphingolipid trafficking. The emission signal of NIR-fluorescent ceramide increases significantly in liposomal environments. NIR-fluorescent ceramide acts as an intracellular trafficking tracer, localizing to regions surrounding the endoplasmic reticulum and Golgi apparatus within a short period, and then translocating to recycling lysosomes over a longer period, thus enabling real-time visualization of sphingolipid trafficking and biological lipid functions in living cells. NIR-fluorescent ceramide also generates metabolic uptake profiles and provides multiple metabolic readouts in human cancer cell lines.
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- No. CAS: 2351937-34-1
- Fòrmula: C33H55N5O5
- Peso molecular:601.82
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Actividad biológica
NIR‐fluorescent ceramide (compound 8) exhibits a fluorescence response property, with its fluorescence emission intensity increasing by approximately 15-fold in liposomes with a phosphatidylcholine/cholesterol ratio of 7:1[1].
NIR-fluorescent ceramide (50 nM; 15 min, 3 h) mainly localizes to the endoplasmic reticulum of A549 cells at 15 min post-treatment, and translocates to recycling lysosomes by 3 h post-treatment[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human A549 lung cancer cells
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Concentration:50 nM
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Incubation Time:15 min (imaging); 3 h (imaging)
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Result:Showed high co-localization with ER Tracker Green (co-localization coefficient R = 0.91) and low co-localization with LysoTracker Blue (R = 0.26) at 15 min post-treatment.
Showed increased co-localization with LysoTracker Blue (R = 0.75) and reduced co-localization with ER Tracker Green (R = 0.49) at 3 h post-treatment.
Chemical Information
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No. CAS 2351937-34-1
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Peso molecular 601.82
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Fòrmula C33H55N5O5
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SMILES
CC1(C)N=C2C(NCCCCCC(N[C@@H]([C@@H](O)/C=C/CCCCCCCCCCCCC)CO)=O)=CC=C([N+]([O-])=O)C2=N1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
[1]. Benson S, et al. SCOTfluors: Small, Conjugatable, Orthogonal, and Tunable Fluorophores for In Vivo Imaging of Cell Metabolism. Angew Chem Int Ed Engl. 2019;58(21):6911-6915. [Content Brief]
[2]. Munan S, et al. Chronological development of functional fluorophores for bio-imaging. Chem Commun (Camb). 2024;60(5):501-521. Published 2024 Jan 11. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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