(+)-Blebbistatin
Based on 1 publication(s) in Google Scholar
(+)-Blebbistatin is the inactive enantiomer of (-)-Blebbistatin (HY-13441). (-)-Blebbistatin is a selective inhibitor of non-muscle myosin II ATPase activity.
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- Pureza : 98.17%
- No. CAS: 1177356-70-5
- Fòrmula: C18H16N2O2
- Peso molecular:292.33
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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HY-13441
(-)-BlebbistatinMyosin II ATPase Inhibitor -
HY-10108
LY294002PI3K Inhibitor -
HY-13813
BlebbistatinMyosin II Inhibitor -
HY-109037
MavacamtenCardiac Myosin Modulator -
HY-16690
BTSMyosin S1 ATPase Inhibitor -
HY-14233
Omecamtiv mecarbilCardiac Myosin Activator -
HY-100932
ML-9Akt Kinase Inhibitor -
HY-107657R
(+)-Blebbistatin (Standard)Reference Standard -
HY-139465
AficamtenCardiac Myosin Inhibitor -
HY-100685
MS-444HuR (ELAVL1) Inhibitor -
HY-15417
ML-7 hydrochlorideMLCK Inhibitor -
HY-109037R
Mavacamten (Standard)Cardiac Myosin Modulator
Publications Citing Use of MedChemExpress (MCE) (+)-Blebbistatin
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Actividad biológica
Descripciòn
In Vitro
(+)-Blebbistatin (5 μM; 20 minutes), the inactive enantiomer, does not prevent thrombin-induced inhibition of intercellular Ca2+ wave propagation in primary cultured bovine corneal endothelial cells[3].
(+)-Blebbistatin (5 μM; 20 minutes) does not prevent thrombin-induced inhibition of hemichannel-mediated Lucifer Yellow uptake in primary cultured bovine corneal endothelial cells[3].
(+)-Blebbistatin (5 μM; 20 minutes) does not prevent thrombin-induced inhibition of mechanical stimulation-evoked ATP release in primary cultured bovine corneal endothelial cells[3].
(+)-Blebbistatin (5 μM; 20 minutes) does not prevent thrombin-induced inhibition of gap junctional intercellular communication in primary cultured bovine corneal endothelial cells[3].
(+)-Blebbistatin (0-100 μM) does not inhibit actin-activated ATPase activity of purified human platelet nonmuscle myosin II S1 at concentrations up to 100 μM[5].
(+)-Blebbistatin (50-100 μM) does not inhibit actin-activated ATPase activity of purified skeletal muscle myosin II-HMM, nonmuscle myosin IIA-HMM, nonmuscle myosin IIB-HMM, smooth muscle myosin II-HMM, myosin Ib, myosin Va-S1, or myosin X-HMM at concentrations of 50 μM and 100 μM[5].
(+)-Blebbistatin (0-160 μM) does not inhibit cytokinesis or induce binucleate cell formation in HeLa cells at concentrations up to 160 μM[5].
(+)-Blebbistatin (100 μM) does not inhibit cytokinesis in Xenopus tissue culture cells at a concentration of 100 μM[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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No. CAS 1177356-70-5
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Appearance Solid
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Peso molecular 292.33
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Fòrmula C18H16N2O2
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Color Light yellow to yellow
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SMILES
O[C@]12C(N(C3=CC=CC=C3)CC2)=NC4=CC=C(C)C=C4C1=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Sci China Life Sci
2022 Feb;65(2):341-361. PMID: 34047913
Solvente y solubilidad
In Vitro:
DMSO : 120 mg/mL (410.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 6 mg/mL (20.52 mM); Clear solution
This protocol yields a clear solution of ≥ 6 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (60.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (294 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[3]. Ponsaerts R, et al. The myosin II ATPase inhibitor blebbistatin prevents thrombin-induced inhibition of intercellular calcium wave propagation in corneal endothelial cells. Investigative ophthalmology & visual science. 2008 Nov;49(11):4816-27. [Content Brief]
[5]. Straight AF, et al. Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor. Science (New York, N.Y.). 2003 Mar 14;299(5613):1743-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4208 mL | 17.1040 mL | 34.2079 mL | 85.5198 mL |
| 5 mM | 0.6842 mL | 3.4208 mL | 6.8416 mL | 17.1040 mL | |
| 10 mM | 0.3421 mL | 1.7104 mL | 3.4208 mL | 8.5520 mL | |
| 15 mM | 0.2281 mL | 1.1403 mL | 2.2805 mL | 5.7013 mL | |
| 20 mM | 0.1710 mL | 0.8552 mL | 1.7104 mL | 4.2760 mL | |
| 25 mM | 0.1368 mL | 0.6842 mL | 1.3683 mL | 3.4208 mL | |
| 30 mM | 0.1140 mL | 0.5701 mL | 1.1403 mL | 2.8507 mL | |
| 40 mM | 0.0855 mL | 0.4276 mL | 0.8552 mL | 2.1380 mL | |
| 50 mM | 0.0684 mL | 0.3421 mL | 0.6842 mL | 1.7104 mL | |
| 60 mM | 0.0570 mL | 0.2851 mL | 0.5701 mL | 1.4253 mL | |
| 80 mM | 0.0428 mL | 0.2138 mL | 0.4276 mL | 1.0690 mL | |
| 100 mM | 0.0342 mL | 0.1710 mL | 0.3421 mL | 0.8552 mL |
(+)-Blebbistatin Related Classifications
HY-107657 Related Classifications
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Keywords
- (+)-Blebbistatin
- 1177356-70-5
- Myosin
- myosin II motor domain
- HeLa cells
- cytoskeletal diseases
- myosin light chain phosphorylation
- Xenopus tissue culture cells
- myosin II
- primary cultured bovine corneal endothelial cells
- corneal endothelial cells
- actin-activated ATPase activity
- skeletal muscle myosin II subfragment-1
- Inhibitor
- inhibitor
- inhibit