RO-5963
Based on 1 publication(s) in Google Scholar
RO-5963 is a dual p53-MDM2 and p53-MDMX inhibitor with IC50s of ~17 nM and ~24 nM, respectively.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.19%
- No. CAS: 1416663-77-8
- Fòrmula: C24H21ClF2N4O5
- Peso molecular:518.90
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) RO-5963
More
Actividad biológica
Descripciòn
IC50 & Target
IC50: ~17 nM (p53-MDM2), ~24 nM (p53-MDMX)[1]
In Vitro
RO-5963 (10-20 μM; 48 hours) shows much higher apoptotic activity than Nutlin in both MCF7 and ZR75-30 cell lines[1].
RO-5963 (10 μM, 24 hours) effectively activats p53 and elevates p21 and MDM2 levels[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:ZR75-30, MCF7 cells
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Concentration:10, 20 μM
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Incubation Time:48 hours
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Result:Potently showed apoptotic activity.
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Cell Line:LNCap, U2OS, RKO, A489, 22Rv1, HCT116, H460, LOX, MCF7, A549, G401 cells
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Concentration:10 μM
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Incubation Time:24 hour
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Result:Effectively activated p53 and elevated p21 and MDM2 levels.
Chemical Information
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No. CAS 1416663-77-8
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Appearance Solid
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Peso molecular 518.90
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Fòrmula C24H21ClF2N4O5
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Color Light yellow to yellow
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SMILES
OCC(CO)NC(C(C1=CC(F)=C(F)C=C1)N2C(/C(NC2=O)=C\C(C3=CC=C4Cl)=CNC3=C4C)=O)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Mol Syst Biol
A split intein and split luciferase-coupled system for detecting protein-protein interactions. [Abstract]2025 Feb;21(2):107-125. PMID: 39668253
Solvente y solubilidad
In Vitro:
DMSO : 150 mg/mL (289.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.75 mg/mL (7.23 mM); Clear solution
This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.75 mg/mL (7.23 mM); Clear solution
This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.9272 mL | 9.6358 mL | 19.2715 mL | 48.1788 mL |
| 5 mM | 0.3854 mL | 1.9272 mL | 3.8543 mL | 9.6358 mL | |
| 10 mM | 0.1927 mL | 0.9636 mL | 1.9272 mL | 4.8179 mL | |
| 15 mM | 0.1285 mL | 0.6424 mL | 1.2848 mL | 3.2119 mL | |
| 20 mM | 0.0964 mL | 0.4818 mL | 0.9636 mL | 2.4089 mL | |
| 25 mM | 0.0771 mL | 0.3854 mL | 0.7709 mL | 1.9272 mL | |
| 30 mM | 0.0642 mL | 0.3212 mL | 0.6424 mL | 1.6060 mL | |
| 40 mM | 0.0482 mL | 0.2409 mL | 0.4818 mL | 1.2045 mL | |
| 50 mM | 0.0385 mL | 0.1927 mL | 0.3854 mL | 0.9636 mL | |
| 60 mM | 0.0321 mL | 0.1606 mL | 0.3212 mL | 0.8030 mL | |
| 80 mM | 0.0241 mL | 0.1204 mL | 0.2409 mL | 0.6022 mL | |
| 100 mM | 0.0193 mL | 0.0964 mL | 0.1927 mL | 0.4818 mL |
RO-5963 Related Classifications
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