Solasonine
Based on 3 publication(s) in Google Scholar
Solasonine is a ferroptosis inducer which can be isolated from Solanum melongena that has anti-infection, anti-cancer, and neurogenesis promoting properties. Solasonine promotes ferroptosis of HCC cells via destruction of the glutathione redox system induced by inhibiting GPX4, and can be used for cancer research.
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- Pureza: 99.0%
- No. CAS: 19121-58-5
- Fòrmula: C45H73NO16
- Peso molecular:884.06
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Publications Citing Use of MedChemExpress (MCE) Solasonine
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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Flow Cytometry
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WB
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IF
Actividad biológica
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GPX4 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KB | ED50 |
11.4 μg/mL
Compound: solasonine
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 8450317] |
| PLC-PRF-5 | ED50 |
7.88 μg/mL
Compound: solasonine
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Cytotoxicity against human PLC/PRF/5 cells
Cytotoxicity against human PLC/PRF/5 cells
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[PMID: 8450317] |
Solasonine (2.5-50 ng/mL, 24 h) inhibits cell viability of HepG2 and HepRG in a dose-dependent manner with IC50s of about 15 ng/mL for both HepG2 and HepRG[2].
Solasonine (15 ng/mL, 24 h) significantly increases the proportions of apoptotic HepG2 and HepRG cells from about 4% to more than 20 %[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 19121-58-5
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Appearance Solid
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Peso molecular 884.06
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Fòrmula C45H73NO16
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Color White to light yellow
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SMILES
C[C@H](CC1)CN[C@]21O[C@@]3([H])C[C@@]4([H])[C@]5([H])CC=C6C[C@@H](O[C@@]7([H])[C@H](O[C@@]8([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O8)[C@@H](O[C@@]9([H])[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O9)[C@@H](O)[C@@H](CO)O7)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@@]3([H])[C@@H]2C
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Front Oncol
Solasonine Causes Redox Imbalance and Mitochondrial Oxidative Stress of Ferroptosis in Lung Adenocarcinoma. [Abstract]2022 May 18:12:874900. PMID: 35664792
Solasonine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2022 May 18:12:874900. [Abstract]
The cell viability of Calu-1 and A549 cells was measured using the CCK-8 assay after SS treatment for 24 h.
Solasonine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2022 May 18:12:874900. [Abstract]
Microscope observation of Calu-1 or A549 cells treated with 10, 15, 20 μM or 20, 25, 30 μM of Solasonine (SS) for 24 h, respectively.
Solasonine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2022 May 18:12:874900. [Abstract]
Flow cytometry were used to detect ROS production in Calu-1 or A549 cells treated for 6 h with 10, 15, 20 μM or 20, 25, 30 μM Solasonine (SS) and pretreated with or without Fer-1 (1 μM).
Solasonine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2022 May 18:12:874900. [Abstract]
Western blot analysis was used to detect the expressions of SLC7A11 and GPX4 in Calu-1 cells, which were treated with 10, 15, 20 μM Solasonine (SS) for 6 h.
Solasonine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2022 May 18:12:874900. [Abstract]
Fluorescence microscopy was used to detect the lipid ROS production in Calu-1cells, which were treated with 20 μM Solasonine (SS) for 6 h and pretreated with or without Trolox (100 mM) or Mito TEMPO (10 mM).
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Adv Biol (Weinh)
Solasonine Restores Sensitivity of Gastric Cancer to NK Cells through DNA Demethylation of MICA. [Abstract]2025 Aug 7:e00793. PMID: 40772533 -
Solvente y solubilidad
DMSO : 100 mg/mL (113.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (277 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
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- Korean - KR (392 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Ding X, et al. Purification, antitumor activity in vitro of steroidal glycoalkaloids from black nightshade (Solanum nigrum L.). Food Chem. 2013 Nov 15;141(2):1181-6. [Content Brief]
[2]. Jin M, et al. Solasonine promotes ferroptosis of hepatoma carcinoma cells via glutathione peroxidase 4-induced destruction of the glutathione redox system. Biomed Pharmacother. 2020 Sep;129:110282. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1311 mL | 5.6557 mL | 11.3114 mL | 28.2786 mL |
| 5 mM | 0.2262 mL | 1.1311 mL | 2.2623 mL | 5.6557 mL | |
| 10 mM | 0.1131 mL | 0.5656 mL | 1.1311 mL | 2.8279 mL | |
| 15 mM | 0.0754 mL | 0.3770 mL | 0.7541 mL | 1.8852 mL | |
| 20 mM | 0.0566 mL | 0.2828 mL | 0.5656 mL | 1.4139 mL | |
| 25 mM | 0.0452 mL | 0.2262 mL | 0.4525 mL | 1.1311 mL | |
| 30 mM | 0.0377 mL | 0.1885 mL | 0.3770 mL | 0.9426 mL | |
| 40 mM | 0.0283 mL | 0.1414 mL | 0.2828 mL | 0.7070 mL | |
| 50 mM | 0.0226 mL | 0.1131 mL | 0.2262 mL | 0.5656 mL | |
| 60 mM | 0.0189 mL | 0.0943 mL | 0.1885 mL | 0.4713 mL | |
| 80 mM | 0.0141 mL | 0.0707 mL | 0.1414 mL | 0.3535 mL | |
| 100 mM | 0.0113 mL | 0.0566 mL | 0.1131 mL | 0.2828 mL |
Solasonine Related Classifications
HY-N0070 Related Classifications
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