ThioLox
Based on 1 Customer Validation
ThioLox is a competitive 15-lipoxygenase-1 (15-LOX-1) inhibitor with a Ki of 3.30 μM. ThioLox shows anti-inflammatory and neuroprotective properties.
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- Pureza: 99.74%
- No. CAS: 1202193-89-2
- Fòrmula: C15H18N2OS
- Peso molecular:274.38
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Immunology/Inflammation Compound Library
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Metabolism/Protease Compound Library
HY-L012
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Ferroptosis Compound Library
HY-L051
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Pyroptosis Compound Library
HY-L059
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Neuroprotective Compound Library
HY-L070
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Lipid Metabolism Compound Library
HY-L091
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Metabolic Enzyme Compound Library
HY-L146
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
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Anti-Fibrosis Compound Library
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High-Throughput Bioactive Compound Library
HY-L205
Actividad biológica
Ki: 3.30 μM (15-LOX-1)[1]
ThioLox shows competitive inhibition thus indicating non-covalent interaction with the active site of the 15-LOX-1[1].
ThioLox (50 μM; 24 h) inhibits interleukin (IL-1β, IL-6, IL-8, IL-12b, TNFα and iNOS) expression in precision-cut lung slices (PCLS)[1].
ThioLox (10 μM; 16 h) prevent lipid peroxidation and mitochondrial superoxide formation in neuronal cells[1].
ThioLox (5-20 μM; 14-16 h) protects HT-22 cells against glutamate toxicity and significantly reduces neuronal cell death[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Precision-cut lung slices (PCLS)
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Concentration:1, 5, 10, 25 and 50 μM
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Incubation Time:In combination with 10 μM linoleic acid for 20 h and stimulated with 10 ng/mL LPS for the last 4 h.
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Result:Provided almost 50% inhibition of the expression of the pro-inflammatory genes IL-1β, IL-6, IL-8, IL-12b, TNFα and iNOS at 50 μM.
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Cell Line:Neuronal HT-22 cells
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Concentration:5, 10 and 20 μM
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Incubation Time:14-16 h
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Result:Significantly reduced neuronal cell death against glutamate toxicity.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 1202193-89-2
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Appearance Solid
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Peso molecular 274.38
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Fòrmula C15H18N2OS
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Color Off-white to light yellow
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SMILES
O=C(C1=C(N)SC(C2=CC=CC=C2)=C1)NCCCC
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (364.46 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6446 mL | 18.2229 mL | 36.4458 mL | 91.1145 mL |
| 5 mM | 0.7289 mL | 3.6446 mL | 7.2892 mL | 18.2229 mL | |
| 10 mM | 0.3645 mL | 1.8223 mL | 3.6446 mL | 9.1115 mL | |
| 15 mM | 0.2430 mL | 1.2149 mL | 2.4297 mL | 6.0743 mL | |
| 20 mM | 0.1822 mL | 0.9111 mL | 1.8223 mL | 4.5557 mL | |
| 25 mM | 0.1458 mL | 0.7289 mL | 1.4578 mL | 3.6446 mL | |
| 30 mM | 0.1215 mL | 0.6074 mL | 1.2149 mL | 3.0372 mL | |
| 40 mM | 0.0911 mL | 0.4556 mL | 0.9111 mL | 2.2779 mL | |
| 50 mM | 0.0729 mL | 0.3645 mL | 0.7289 mL | 1.8223 mL | |
| 60 mM | 0.0607 mL | 0.3037 mL | 0.6074 mL | 1.5186 mL | |
| 80 mM | 0.0456 mL | 0.2278 mL | 0.4556 mL | 1.1389 mL | |
| 100 mM | 0.0364 mL | 0.1822 mL | 0.3645 mL | 0.9111 mL |
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