Glutor
Based on 1 Customer Validation
Glutor is a selective GLUT 1/2/3 inhibitor that can suppress glucose uptake. Glutor can inhibit glycolysis and has anti-tumor activity, inducing cell apoptosis.
For research use only. We do not sell to patients.
- Purity: 99.54%
- CAS No.: 2561471-22-3
- Formula: C31H32N6O3
- Molecular Weight:536.62
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Glutor reduced the uptake of 2-DG with similar efficacy in different cancer cell lines, such as HCT116 (IC50 = 10.8 nM), UM-UC-3 (IC50 = 8.3 nM), UO-31 (IC50 = 3.6 nM), and MIA PaCa-2 (IC50 = 1.1 nM)[1].
Glutor effectively decreases glycolysis in HCT116 cells, thereby preventing the reduction of oxidative phosphorylation rates after glucose addition[1].
Glutor inhibits the proliferation of malignant cell lines without harming the growth of non-malignant peripheral blood mononuclear cells (PBMCs) and IMR-90 embryonic lung cells[1].
Glutor (0.5μM, 24-48h) suppresses the glucose uptake-induced upregulation of GLUT-1 and GLUT-3 expression[1].
Glutor (0.0001-1μM, 24h) inhibits the number of DL cells in a time- and dose-dependent manner, with an Glutor reduced the uptake of 2-DG with similar efficacy in different cancer cell lines, such as HCT116 (IC50 = 10.8 nM), UM-UC-3 (IC50 = 8.3 nM), UO-31 (IC50 = 3.6 nM), and MIA PaCa-2 (IC50 = 1.1 nM)[1].
Glutor effectively decreases glycolysis in HCT116 cells, thereby preventing the reduction of oxidative phosphorylation rates after glucose addition[1].
Glutor inhibits the proliferation of malignant cell lines without harming the growth of non-malignant peripheral blood mononuclear cells (PBMCs) and IMR-90 embryonic lung cells[1].
Glutor (0.5 μM, 24-48 h) suppresses the glucose uptake-induced upregulation of GLUT-1 and GLUT-3 expression[1].
Glutor (0.0001-1 μM, 24 h) inhibits the number of DL cells in a time- and dose-dependent manner, with an IC50 of 0.01 μM, showing no cytotoxicity to thymocytes[2].
Glutor (0.01-1 μM, 24 h) induces apoptosis in DL cells[2].
Glutor (0.01-1 μM, 24 h) links tumor inhibition to the regulation of cell survival and metabolic regulatory molecules[2].
Glutor (0.01-1 μM, 2 h) increases the expression of reactive oxygen species (ROS) in DL cells[2].
Glutor (0.01-1 μM, 24 h) triggers depolarization of the mitochondrial membrane, disrupting the pH homeostasis of DL cells and altering their chemical sensitivity[2].
of 0.01 μM, showing no cytotoxicity to thymocytes[2].
Glutor (0.01-1 μM, 24 h) induces apoptosis in DL cells[2].
Glutor (0.01-1 μM, 24 h) links tumor inhibition to the regulation of cell survival and metabolic regulatory molecules[2].
Glutor (0.01-1 μM, 2 h) increases the expression of reactive oxygen species (ROS) in DL cells[2].
Glutor (0.01-1 μM, 24 h) triggers depolarization of the mitochondrial membrane, disrupting the pH homeostasis of DL cells and altering their chemical sensitivity[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:DLD-1
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Concentration:0.5 μM
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Incubation Time:24, 48 h
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Result:Showed under low blood sugar conditions, the levels of GLUT1 and GLUT3 increase.
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Cell Line:DLD-1
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Concentration:0.5 μM
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Incubation Time:24, 48 h
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Result:Showed under low blood sugar conditions, the levels of GLUT1 and GLUT3 increase.
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Cell Line:DL
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Concentration:0.0001, 0.001, 0.01, 0.1, 1μM
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Incubation Time:24 h
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Result:Showed the number of cells decreases in a dose- and time-dependent manner.
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Cell Line:DL
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Concentration:0.01 μM
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Incubation Time:24 h
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Result:Increased the number of cells with typical apoptotic morphological characteristics.
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Cell Line:DL
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Concentration:0.01 μM
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Incubation Time:24 h
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Result:Suppressed the expression of hypoxia-inducible factor 1-alpha (HIF-1a), hexokinase 2 (HK-2), and lactate dehydrogenase A (LDH-A), regulating the expression of cell survival molecules B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X (BAX), and p53. The expression of Bcl-2, transforming growth factor-beta (TGF-b), Cmyc, and heat shock protein 70 (Hsp70) decreased, while the expression of BAX and p53 increased.
Chemical Information
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CAS No. 2561471-22-3
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Appearance Solid
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Molecular Weight 536.62
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Formula C31H32N6O3
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Color White to off-white
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SMILES
O=C([C@@]1(C)N(C(C2=CC(C3=CC=CC=C3)=NN2C1)=O)CC(C=C4)=CC=C4N5CCOCC5)NCC6=CC=CN=C6
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 34 mg/mL (63.36 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (9.32 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Reckzeh ES, et al. Inhibition of Glucose Transporters and Glutaminase Synergistically Impairs Tumor Cell Growth. Cell Chem Biol. 2019;26(9):1214-1228.e25. [Content Brief]
[2]. Mithlesh Kumar Temre， et al. Glutor, a Glucose Transporter Inhibitor, Exerts Antineoplastic Action on Tumor Cells of Thymic Origin: Implication of Modulated Metabolism, Survival, Oxidative Stress, Mitochondrial Membrane Potential, pH Homeostasis, and Chemosensitivity. Front Oncol. 2022 Jun 30:12:925666. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8635 mL | 9.3176 mL | 18.6352 mL | 46.5879 mL |
| 5 mM | 0.3727 mL | 1.8635 mL | 3.7270 mL | 9.3176 mL | |
| 10 mM | 0.1864 mL | 0.9318 mL | 1.8635 mL | 4.6588 mL | |
| 15 mM | 0.1242 mL | 0.6212 mL | 1.2423 mL | 3.1059 mL | |
| 20 mM | 0.0932 mL | 0.4659 mL | 0.9318 mL | 2.3294 mL | |
| 25 mM | 0.0745 mL | 0.3727 mL | 0.7454 mL | 1.8635 mL | |
| 30 mM | 0.0621 mL | 0.3106 mL | 0.6212 mL | 1.5529 mL | |
| 40 mM | 0.0466 mL | 0.2329 mL | 0.4659 mL | 1.1647 mL | |
| 50 mM | 0.0373 mL | 0.1864 mL | 0.3727 mL | 0.9318 mL | |
| 60 mM | 0.0311 mL | 0.1553 mL | 0.3106 mL | 0.7765 mL |
Glutor Related Classifications
HY-158302 Related Classifications
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