Golgicide A
Based on 11 publication(s) in Google Scholar
Golgicide A (GCA) is a potent, highly specific, and reversible inhibitor of the cis-Golgi ADP-ribosylation factor guanine nucleotide exchange factors (ArfGEF) GBF1. Golgicide A drastically reduced replication of coxsackievirus B3 (CVB3) and other human enterovirus species.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.94%
- CAS. Nr.: 1139889-93-2
- Formel: C17H14F2N2
- Molecular Weight:284.30
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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HY-L205
Publications Citing Use of MedChemExpress (MCE) Golgicide A
More- Nature. 2025 Oct;646(8086):913-924. [Abstract]
- Autophagy. 2025 Dec 8. [Abstract]
- Nat Commun. 2025 May 27;16(1):4887. [Abstract]
- Proc Natl Acad Sci U S A. 2025 Jul 15;122(28):e2501347122. [Abstract]
- EMBO J. 2024 Oct;43(20):4625-4655. [Abstract]
- Mol Metab. 2021 Dec;54:101329. [Abstract]
- J Virol. 2024 Jul 23;98(7):e0081324. [Abstract]
- Res Sq. 2026 Jan 9.
- Res Sq. 2024 Nov 06.
- Research Square Preprint. 2024 Jan 18.
- bioRxiv. 2024 Jan 2:2023.12.31.573800. [Abstract]
Customer Validation & Images
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RT-PCR
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WB
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ELISA
Biologische Aktivität
GBF1[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Vero | IC50 |
3.3 μM
Compound: 2, GCA
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Restoration of protein synthesis in african green monkey Vero cells assessed as radioactive aminoacid incorporation after 30 min in presence of shiga toxin
Restoration of protein synthesis in african green monkey Vero cells assessed as radioactive aminoacid incorporation after 30 min in presence of shiga toxin
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[PMID: 19182783] |
Golgicide A (GCA) is a potent and highly effective inhibitor of shiga toxin activity. Golgicide A (GCA) inhibits the effect of shiga toxin on protein synthesis with an IC50 of 3.3 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS. Nr. 1139889-93-2
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Appearance Solid
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Molecular Weight 284.30
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Formel C17H14F2N2
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Color White to off-white
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SMILES
FC1=CC2=C(NC(C3=CC=CN=C3)[C@@]4([H])[C@]2([H])C=CC4)C(F)=C1
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Synonyms
GCA
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (11)
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Journal Impact Factor
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Most Recent
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Nature
2025 Oct;646(8086):913-924. PMID: 40769205 -
Autophagy
Trans-Golgi network-associated noncanonical autophagy depends on the V-ATPase-ATG16L1 axis and mediates IL1B secretion. [Abstract]2025 Dec 8. PMID: 41361996 -
Nat Commun
Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly. [Abstract]2025 May 27;16(1):4887. PMID: 40425567 -
Proc Natl Acad Sci U S A
Monensin suppresses EMT-driven cancer cell motility by inducing Golgi pH-dependent exocytosis of GOLIM4. [Abstract]2025 Jul 15;122(28):e2501347122. PMID: 40632561 -
EMBO J
Interaction of chikungunya virus glycoproteins with macrophage factors controls virion production. [Abstract]2024 Oct;43(20):4625-4655. PMID: 39261662 -
Mol Metab
An inhibitor-mediated beta-cell dedifferentiation model reveals distinct roles for FoxO1 in glucagon repression and insulin maturation. [Abstract]2021 Dec;54:101329. PMID: 34454092
Golgicide A purchased from MedChemExpress. Usage Cited in: Mol Metab. 2021 Dec;54:101329. [Abstract]
Intracellular insulin and proinsulin protein levels after 30 h loperamide treatment +/− 10 μM Golgicide A for the final 6 h, as measured by ELISA.
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J Virol
Enterovirus 3A protein disrupts endoplasmic reticulum homeostasis through interaction with GBF1. [Abstract]2024 Jul 23;98(7):e0081324. PMID: 38904364
Golgicide A purchased from MedChemExpress. Usage Cited in: J Virol. 2024 Jul 23;98(7):e0081324. [Abstract]
Expression of BiP and XBP1s in Golgicide A (GCA)-treated RD cells (1, 3, 10 µM) for 24 h was quantified using qPCR.
Golgicide A purchased from MedChemExpress. Usage Cited in: J Virol. 2024 Jul 23;98(7):e0081324. [Abstract]
RD cells expressing ARF1 Q71L at varying levels were treated with Golgicide A (GCA) (10 µM) 24 h post-transfection. The expression of ARF1 was detected using immunoblotting at 48 h post-transfection.
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bioRxiv
GTPase-activating protein ARAP1 regulates circular dorsal ruffles as a nutrient uptake mechanism in the Hep3B hepatocellular carcinoma cell line. [Abstract]2024 Jan 2:2023.12.31.573800. PMID: 38260345
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (351.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Sáenz JB, et al. Golgicide A reveals essential roles for GBF1 in Golgi assembly and function. Nat Chem Biol. 2009 Mar;5(3):157-65. [Content Brief]
[2]. van der Linden L, et al. Differential effects of the putative GBF1 inhibitors Golgicide A and AG1478 on enterovirus replication. J Virol. 2010 Aug;84(15):7535-42. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.5174 mL | 17.5871 mL | 35.1741 mL | 87.9353 mL |
| 5 mM | 0.7035 mL | 3.5174 mL | 7.0348 mL | 17.5871 mL | |
| 10 mM | 0.3517 mL | 1.7587 mL | 3.5174 mL | 8.7935 mL | |
| 15 mM | 0.2345 mL | 1.1725 mL | 2.3449 mL | 5.8624 mL | |
| 20 mM | 0.1759 mL | 0.8794 mL | 1.7587 mL | 4.3968 mL | |
| 25 mM | 0.1407 mL | 0.7035 mL | 1.4070 mL | 3.5174 mL | |
| 30 mM | 0.1172 mL | 0.5862 mL | 1.1725 mL | 2.9312 mL | |
| 40 mM | 0.0879 mL | 0.4397 mL | 0.8794 mL | 2.1984 mL | |
| 50 mM | 0.0703 mL | 0.3517 mL | 0.7035 mL | 1.7587 mL | |
| 60 mM | 0.0586 mL | 0.2931 mL | 0.5862 mL | 1.4656 mL | |
| 80 mM | 0.0440 mL | 0.2198 mL | 0.4397 mL | 1.0992 mL | |
| 100 mM | 0.0352 mL | 0.1759 mL | 0.3517 mL | 0.8794 mL |
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