HMRef-αMan
HMRef-αMan is a substrate-based green fluorescent probe (Ex/Em=465 nm/515 nm) targeting MAN2C1 (α-mannosidase). HMRef-αMan can be specifically cleaved by MAN2C1 to generate a highly fluorescent product, which thus gets activated to produce green fluorescence in malignant breast tissues, benign lesions and living cancer cells. The signal intensity of HMRef-αMan is directly correlated with MAN2C1 activity, and it can effectively detect tiny breast cancer lesions with a diameter of less than 1 mm. When used in combination with the red-emitting γ-glutamyl transpeptidase (GGT) probe gGlu-2OMe SiR600 (HY-D3188), HMRef-αMan enables precise optical differentiation of breast tissue types via a dual-color imaging strategy. HMRef-αMan has been widely used in the research of breast diseases such as breast cancer, fibroadenoma, phyllodes tumor and various types of papilloma.
For research use only. We do not sell to patients.
- CAS No.: 2484831-02-7
- Formula: C28H26F3NO8
- Molecular Weight:561.50
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
HMRef-αMan (10 μM; 1 h) visualizes α-mannosidase activity in diverse live cultured human cancer cell lines[1].
HMRef-αMan (50 μM; 1-30 min) selectively and time-dependently activates fluorescence in surgically resected human IDC and DCIS tissues, enabling breast cancer detection with 90% sensitivity and 100% specificity[1].
HMRef-αMan (50 μM; 5-30 min) rapidly visualizes (within 15 min) tiny, pathologically confirmed human IDC and DCIS lesions in surgically resected specimens, with fluorescence activation limited to cancerous tissue regions[1].
HMRef-αMan (50 μM; 5-30 min) activates fluorescence in surgically resected human benign breast lesions (FA, PT, ICP, IDP), enabling discrimination from normal tissue with 100% sensitivity and 100% specificity for FA[1].
HMRef-αMan (50 μM; 15 min), when combined with a red-emitting γ-glutamyltranspeptidase probe, enables optical discrimination of surgically resected human normal breast tissue, IDC/DCIS, and FA after 15 min of incubation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2484831-02-7
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Molecular Weight 561.50
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Formula C28H26F3NO8
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SMILES
OC[C@H]1O[C@@H]([C@H]([C@H]([C@@H]1O)O)O)OC2=CC=C3C(OC4=CC(NCC(F)(F)F)=CC=C4C35OCC6=C5C=CC=C6)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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