HMRef-αMan 

HMRef-αMan is a substrate-based green fluorescent probe (Ex/Em=465 nm/515 nm) targeting MAN2C1 (α-mannosidase). HMRef-αMan can be specifically cleaved by MAN2C1 to generate a highly fluorescent product, which thus gets activated to produce green fluorescence in malignant breast tissues, benign lesions and living cancer cells. The signal intensity of HMRef-αMan is directly correlated with MAN2C1 activity, and it can effectively detect tiny breast cancer lesions with a diameter of less than 1 mm. When used in combination with the red-emitting γ-glutamyl transpeptidase (GGT) probe gGlu-2OMe SiR600 (HY-D3188), HMRef-αMan enables precise optical differentiation of breast tissue types via a dual-color imaging strategy. HMRef-αMan has been widely used in the research of breast diseases such as breast cancer, fibroadenoma, phyllodes tumor and various types of papilloma^[1].

HMRef-αMan (10 μM; 1 h) visualizes α-mannosidase activity in diverse live cultured human cancer cell lines^[1].
HMRef-αMan (50 μM; 1-30 min) selectively and time-dependently activates fluorescence in surgically resected human IDC and DCIS tissues, enabling breast cancer detection with 90% sensitivity and 100% specificity^[1].
HMRef-αMan (50 μM; 5-30 min) rapidly visualizes (within 15 min) tiny, pathologically confirmed human IDC and DCIS lesions in surgically resected specimens, with fluorescence activation limited to cancerous tissue regions^[1].
HMRef-αMan (50 μM; 5-30 min) activates fluorescence in surgically resected human benign breast lesions (FA, PT, ICP, IDP), enabling discrimination from normal tissue with 100% sensitivity and 100% specificity for FA^[1].
HMRef-αMan (50 μM; 15 min), when combined with a red-emitting γ-glutamyltranspeptidase probe, enables optical discrimination of surgically resected human normal breast tissue, IDC/DCIS, and FA after 15 min of incubation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

561.50

C₂₈H₂₆F₃NO₈

2484831-02-7

OC[C@H]1O[C@@H]([C@H]([C@H]([C@@H]1O)O)O)OC2=CC=C3C(OC4=CC(NCC(F)(F)F)=CC=C4C35OCC6=C5C=CC=C6)=C2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Fujita K, et al. Rapid and accurate visualization of breast tumors with a fluorescent probe targeting α-mannosidase 2C1[J]. ACS central science, 2020, 6(12): 2217-2227.