eCF309
Based on 1 Customer Validation
eCF309 is a potent and highly selective mTOR inhibitor with an IC50 of 15 nM. eCF309 displays higher selectivity over PI3Ks. eCF309 can be used for the study of breast cancer and prostate cancer.
For research use only. We do not sell to patients.
- Purity: 98.2%
- CAS No.: 2001571-40-8
- Formula: C18H21N7O3
- Molecular Weight:383.40
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | EC50 |
8 nM
Compound: 12
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Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis
Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis
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10.1039/C5MD00493D |
| MDA-MB-231 | EC50 |
72 nM
Compound: 12
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Cytotoxicity against human MDA-MB-231 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
Cytotoxicity against human MDA-MB-231 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
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10.1039/C5MD00493D |
| PC-3 | EC50 |
37 nM
Compound: 12
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Cytotoxicity against human PC3 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
Cytotoxicity against human PC3 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
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10.1039/C5MD00493D |
| T98 | EC50 |
100 nM
Compound: eCF309
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Antiproliferative activity against human T98 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
Antiproliferative activity against human T98 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
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[PMID: 31787462] |
| U-87MG ATCC | EC50 |
100 nM
Compound: eCF309
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Antiproliferative activity against human U87 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
Antiproliferative activity against human U87 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
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[PMID: 31787462] |
eCF309 (0.3-1000 nM; 5 days) exhibits highly potent antiproliferative activity against MCF7, MDA-MB-231 and PC3 cells[1].
eCF309 (0.3-1000 nM; 24 h) causes G0/G1 cell cycle arrest[1].
eCF309 (3-100 nM; 1.5 h) inhibits pP70S6K, pS6 and pAKT levels in MCF7 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF7, MDA-MB-231 and PC3 cells
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Concentration:0.3-1000 nM
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Incubation Time:5 days
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Result:Exhibits EC50 values upon MCF7, MDA-MB-231 and PC3 cells viability of 8.4 nM, 72 nM and 37 nM, respectively.
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Cell Line:MCF7, MDA-MB-231 and PC3 cells
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Concentration:0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM, 300 nM, 1000 nM
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Incubation Time:24 h
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Result:Caused G0/G1 cell cycle arrest.
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Cell Line:MCF7 cells
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Concentration:3 nM, 10 nM, 30 nM, 100 nM
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Incubation Time:For 30 min, followed by 1 h of serum stimulation
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Result:Mediated a dose-dependent reduction of the pP70S6K, pS6 and pAKT levels in MCF7 cells, achieving almost complete inhibition of the phosphorylation of these targets at 30 nM.
Chemical Information
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CAS No. 2001571-40-8
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Appearance Solid
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Molecular Weight 383.40
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Formula C18H21N7O3
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Color White to off-white
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SMILES
NC1=C2C(N(CC(OCC)OCC)N=C2C3=CC=C(OC(N)=N4)C4=C3)=NC=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 1 mg/mL (2.61 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6082 mL | 13.0412 mL | 26.0824 mL | 65.2061 mL |
eCF309 Related Classifications
HY-128027 Related Classifications
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