Cat. No.: HY-N10618

Epilactose

Name: Epilactose
Brand: MedChemExpress
SKU: HY-N10618
Price: 174.00 USD
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Epilactose is an orally active prebiotic disaccharide. Epilactose resists gastrointestinal digestion, stimulates the intestinal epithelium, and induces myosin regulatory light chain phosphorylation, activating the perijunctional actin-myosin ring and paracellular transport. Epilactose produces short-chain fatty acids and organic acids through cecal microbial fermentation, induces paracellular calcium and iron absorption, lowers cecal pH, and increases cecal calcium and magnesium solubility. Epilactose inhibits hepatic cholesterol synthesis. Epilactose can be used for research on osteopenia, colon cancer, Crohn's disease, post-gastrectomy osteopenia and anemia, and obesity.

For research use only. We do not sell to patients.

Purity : 99.89%
CAS No.: 50468-56-9
Formula: C₁₂H₂₂O₁₁
Molecular Weight:342.30

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Epilactose Chemical Structure

CAS No.: 50468-56-9

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Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Epilactose (1% (w/v); 68 h) supports the growth of human-derived Bifidobacterium species in vitro, with B. catenulatum JCM1194 showing the highest growth level among the tested strains (1.068 at 620 nm)^[6].
Epilactose (50-100 mM; 20 min) increases paracellular Ca absorption in rat small intestinal segments in a dose-dependent manner^[1].
Epilactose (50-100 mM; 20 min) induces MLC phosphorylation in rat small intestinal mucosa without affecting the association of tight junction proteins with the actin cytoskeleton^[1].
The promoting effect of Epilactose (100 mM; 20 min) on Ca absorption in rat small intestinal segments is inhibited by MLCK and ROCK inhibitors, but not by PLC or tyrosine kinase inhibitors^[1].
Epilactose (0-80 mM; 3 h) decreases the transepithelial electrical resistance of Caco-2 cell monolayers in a dose- and time-dependent manner at 40 and 80 mM, indicating increased paracellular transport through physiological regulation of tight junctions^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Epilactose (50 g/kg diet; diet; daily; 15 days) increases calcium and magnesium absorption, elevates cecal short-chain fatty acid and succinate levels, and tends to lower plasma total and LDL+VLDL cholesterol in rats^[2].
Epilactose (4.76 g/kg of BW per day; diet; ad libitum; 15 days) in healthy rats increases cecal wall and content weights, lowers cecal pH, proliferates lactobacilli and bifidobacteria, and inhibits the conversion of primary to secondary bile acids^[3].
Epilactose (50 g/kg diet; p.o.; daily; 30 days) ameliorates postgastrectomy osteopenia and completely restores anemia through the promotion of intestinal Ca and Fe absorption in gastrectomized rats^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Wistar-ST (Male, 4-week-old)^[2]
Dosage:

50 g/kg diet
Administration:

diet; daily; 15 days
Result:

Increased cecal wall weight to 0.38 g/100 g B.W. and cecal content weight to 2.24 g/100 g B.W.
Decreased cecal pH to 6.62.
Increased apparent calcium absorption compared to control and lactose groups.
Increased cecal calcium solubility to 32.6%.
Increased cecal magnesium solubility to 79.2%.
Decreased cecal zinc solubility to 4.9%.
Increased cecal acetate to 275 μmol, propionate to 127 μmol, n-butyrate to 46.2 μmol, total SCFAs to 447 μmol, succinate to 185 μmol, lactate to 44.9 μmol, and total organic acids to 677 μmol per whole cecal contents.
Tended to lower plasma total cholesterol and LDL+VLDL cholesterol levels.

Animal Model:

Wistar-ST rats (male, 4 weeks old, 113.6 g)^[3]
Dosage:

4.76 g/kg of BW per day
Administration:

diet; ad libitum; 15 days
Result:

Achieved a body weight gain of 104.0 g over 15 days.
Recorded a total food intake of 257.7 g over 15 days.
Increased cecal wall weight to 3.8 g/kg of BW.
Increased cecal content weight to 22.9 g/kg of BW.
Lowered cecal content pH to 6.61.
Increased the number of cecal lactobacilli significantly compared to control.
Reached a logarithmic cecal bifidobacteria number of 7.80 copies/g.
Resulted in cecal α-MCA of 20.63 μmol/g of dry feces, β-MCA of 35.78 μmol/g, and total primary bile acids of 63.99 μmol/g.
Resulted in cecal deoxycholic acid of 14.67 μmol/g, lithocholic acid of 4.88 μmol/g, and total secondary bile acids of 19.55 μmol/g.
Achieved a ratio of primary to secondary bile acids of 5.41.

Animal Model:

Sprague-Dawley (male, 4 weeks old, total gastrectomy)^[5]
Dosage:

50 g/kg diet
Administration:

p.o.; daily; 30 days
Result:

Increased Ca absorption rate in gastrectomized rats above that of sham rats fed the control diet.
Restored gastrectomy-impaired Fe absorption.
Reversed plasma Ca concentration to 9.22 mg/dL compared to 8.86 mg/dL in control diet.
Partially recovered plasma Fe concentration to 26.2 mg/dL compared to 17.5 mg/dL in control diet.
Increased femoral dry weight to 203 mg/rat compared to 168 mg/rat in control diet.
Increased femoral Ca content to 53.6 mg/whole femur compared to 40.8 mg/whole femur in control diet.
Increased bone strength compared to control diet.
Increased hematocrit and hemoglobin concentration on day 15 compared to control diet.
Recovered hematocrit and hemoglobin to levels comparable to sham rats on day 30.
Increased cecal tissue and content weights compared to control group.
Decreased pH of cecal contents compared to control group.
Increased soluble Ca concentration in cecal contents compared to control diet.
Increased soluble Fe concentration in cecal contents compared to control diet.
Increased acetic acid pool to 365 μmol/whole cecal content, propionic acid pool to 136 μmol/whole cecal content, butyric acid pool to 60.0 μmol/whole cecal content, and total SCFA pool to 562 μmol/whole cecal content compared to sham rats fed the epilactose diet.

Related Protocols :

Chemical Information

CAS No. 50468-56-9
Appearance Solid
Molecular Weight 342.30
Formula C₁₂H₂₂O₁₁
Color White to light yellow
SMILES

O=C[C@@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@@H]1O[C@@H]([C@@H]([C@@H]([C@H]1O)O)O)CO
Structure Classification
- Saccharides
- Polysaccharides
Initial Source
- Animals
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (295 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Suzuki T, et al. The nondigestible disaccharide epilactose increases paracellular Ca absorption via rho-associated kinase- and myosin light chain kinase-dependent mechanisms in rat small intestines. Journal of agricultural and food chemistry. 2010 Feb 10;58(3):1927-32.

[2]. Nishimukai M, et al. Effects of epilactose on calcium absorption and serum lipid metabolism in rats. Journal of agricultural and food chemistry. 2008 Nov 12;56(21):10340-5.

[3]. Watanabe J, et al. Prebiotic properties of epilactose. Journal of dairy science. 2008 Dec;91(12):4518-26.

[4]. Cardoso BB, et al. Epilactose as a Promising Butyrate-Promoter Prebiotic via Microbiota Modulation. Life (Basel, Switzerland). 2024 May 18;14(5):643. [Content Brief]

[5]. Suzuki T, et al. Ingestion of epilactose, a non-digestible disaccharide, improves postgastrectomy osteopenia and anemia in rats through the promotion of intestinal calcium and iron absorption. Journal of agricultural and food chemistry. 2010 Oct 13;58(19):10787-92.

[6]. Ito S, et al. Enzymatic properties of cellobiose 2-epimerase from Ruminococcus albus and the synthesis of rare oligosaccharides by the enzyme. Applied microbiology and biotechnology. 2008 Jun;79(3):433-41.

[7]. Chen Z, et al. Characterization of an epilactose-producing cellobiose 2-epimerase from Clostridium sp. TW13 and reutilization of waste milk. Food chemistry. 2025 Jul 15;480:143948.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Epilactose