Indole-4-carboxaldehyde
Based on 1 Customer Validation
Indole-4-carboxaldehyde is an ergot alkaloid precursor that regulates glycosylation and inflammation. Indole-4-carboxaldehyde upregulates Glo-1, inhibits MGO-induced NF-κB activation, and suppresses MGO-induced expression of TNF-α and IFN-γ. Indole-4-carboxaldehyde inhibits MGO-induced formation of advanced glycation end products (AGE) and expression of their receptor (RAGE). Indole-4-carboxaldehyde is a core metabolite produced in the pedicels of Summer Black grapes after exogenous gibberellin treatment, and it directly promotes fruit enlargement and fruit set of Summer Black grapes. Indole-4-carboxaldehyde can be used in studies related to hepatic steatosis and plant growth regulation.
For research use only. We do not sell to patients.
- Purity : 99.32%
- CAS No.: 1074-86-8
- Formula: C9H7NO
- Molecular Weight:145.16
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
NF-κB |
TNF-α |
In Vitro
Indole-4-carboxaldehyde (ST-14C) (50-200 µM; 24 h) induces the proliferation of HepG2 cells at concentrations of 50 µM and 100 µM[1].
Indole-4-carboxaldehyde (25-200 µM; 6-24 h) induces a dose-dependent increase in Glo-1 mRNA and protein expression, and enhances the fluorescence intensity of Glo-1 in HepG2 cells[1].
Indole-4-carboxaldehyde reacts with 4-substituted thiosemicarbazides in refluxing ethanol to form thioformamide derivatives[2].
Indole-4-carboxaldehyde shows significant upregulation and directly promotes fruit enlargement and fruit set of the pedicels of Summer Black grapes treated twice with exogenous gibberellin[3].
Indole-4-carboxaldehyde (100 µM; 2 h pre-incubation prior to 4 h MGO exposure) attenuates the expression of TNF-α, IFN-γ and RAGE mRNA in MGO-induced HepG2 cells, and inhibits the nuclear translocation of NF-κB p65[1].
Indole-4-carboxaldehyde (100 µM; 2 h pre-incubation prior to 24 h MGO exposure) reduces methylglyoxal-induced secretion of TNF-α and IFN-γ in HepG2 cells and attenuates the formation of AGE[1].
Indole-4-carboxaldehyde (100 µM; 2 h pre-incubation prior to 4-24 h MGO exposure) fails to attenuate MGO-induced IFN-γ mRNA expression and AGE formation in Glo-1-knockdown HepG2 cells, indicating that these effects are mediated by Glo-1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HepG2 human hepatocyte cell line
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Concentration:50, 100, 200 µM
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Incubation Time:24 h
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Result:Significantly increased HepG2 cell proliferation at 50 µM and 100 µM.
Did not increase cell proliferation relative to control at 200 µM.
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Cell Line:HepG2 human hepatocyte cell line
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Concentration:100 µM
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Incubation Time:2 h pre-incubation prior to 4 h MGO (0.25 mM) exposure
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Result:Significantly reduced the MGO-induced increase in TNF-α and IFN-γ mRNA expression, returning levels close to untreated control values.\nReduced MGO-induced RAGE mRNA levels to near untreated control values.
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Cell Line:HepG2 human hepatocyte cell line
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Concentration:100 µM
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Incubation Time:2 h pre-incubation prior to 24 h MGO (0.25 mM) exposure
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Result:Significantly reduced the MGO-induced increase in secreted TNF-α and IFN-γ levels, returning levels close to untreated control values.
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Cell Line:HepG2 human hepatocyte cell line
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Concentration:100 µM
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Incubation Time:2 h pre-incubation prior to 4 h MGO (0.25 mM) exposure
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Result:Prevented MGO-induced nuclear translocation of p65 and restored cytosolic p65 levels to near control values.
Chemical Information
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CAS No. 1074-86-8
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Appearance Solid
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Molecular Weight 145.16
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Formula C9H7NO
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Color Gray to brown
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SMILES
O=CC1=CC=CC2=C1C=CN2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (344.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
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Liver Histomorphometry
Liver histomorphometry is a quantitative histological approach used to measure structural alterations in hepatic tissue, including parenchymal loss, steatosis, fibrosis, and vascular remodeling, by combining stained tissue section analysis with stereological or computerized image-based measurements. Classical morphometric frameworks quantify volume fractions of liver compartments and fibrotic regions using systematic sampling and image analysis, enabling objective comparison of pathological changes across experimental groups. These approaches are widely applied in liver cirrhosis and fibrosis studies to reduce subjectivity in histological scoring and improve reproducibility of tissue evaluation. Recent methodological advances integrate automated image analysis and radiomics-based extraction of histological features from standard liver stains (e. g. , H&E and fibrotic stains), enabling quantitative correlation between morphometric features and fibrosis stages in non-alcoholic fatty live
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.8889 mL | 34.4447 mL | 68.8895 mL | 172.2237 mL |
| 5 mM | 1.3778 mL | 6.8889 mL | 13.7779 mL | 34.4447 mL | |
| 10 mM | 0.6889 mL | 3.4445 mL | 6.8889 mL | 17.2224 mL | |
| 15 mM | 0.4593 mL | 2.2963 mL | 4.5926 mL | 11.4816 mL | |
| 20 mM | 0.3444 mL | 1.7222 mL | 3.4445 mL | 8.6112 mL | |
| 25 mM | 0.2756 mL | 1.3778 mL | 2.7556 mL | 6.8889 mL | |
| 30 mM | 0.2296 mL | 1.1482 mL | 2.2963 mL | 5.7408 mL | |
| 40 mM | 0.1722 mL | 0.8611 mL | 1.7222 mL | 4.3056 mL | |
| 50 mM | 0.1378 mL | 0.6889 mL | 1.3778 mL | 3.4445 mL | |
| 60 mM | 0.1148 mL | 0.5741 mL | 1.1482 mL | 2.8704 mL | |
| 80 mM | 0.0861 mL | 0.4306 mL | 0.8611 mL | 2.1528 mL | |
| 100 mM | 0.0689 mL | 0.3444 mL | 0.6889 mL | 1.7222 mL |
Indole-4-carboxaldehyde Related Classifications
HY-W001288 Related Classifications
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Keywords
- Indole-4-carboxaldehyde
- 1074-86-8
- Glyoxalase (GLO)
- NF-κB
- TNF Receptor
- Drug Intermediate
- HepG2 cells
- Xiahei grapes
- Glyoxalase-1
- Nuclear factor-kappa B
- Receptor for advanced glycation end-products
- hepatic steatosis
- tumor necrosis factor-α
- summer black grapes
- IFN-γ
- advanced glycation end-products
- Inhibitor
- inhibitor
- inhibit