Janelia Fluor® 549 TFA
Based on 1 Customer Validation
Janelia Fluor 549 TFA (JF549 TFA) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
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- Purity : 85.20%
- CAS No.: 2245946-45-4
- 화학식: C29H23F3N2O7
- 분자량:568.50
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보관:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
Janelia Fluor fluorescent dyes do not inherently possess HaloTag-binding activity; however, reactive groups serve as reaction sites for covalent coupling-via chemical synthesis-to an amino-functionalized HaloTag chloroalkane ligand scaffold, thereby yielding a fluorescent HaloTag ligand.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
571
Excitation (Ex)
549
Chemical Information
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CAS No. 2245946-45-4
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Appearance Solid
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분자량 568.50
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화학식 C29H23F3N2O7
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Color Light brown to brown
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SMILES
O=C(C1=CC=C(C(O)=O)C(C2=C3C=CC(N4CCC4)=CC3=[O+]C5=C2C=CC(N6CCC6)=C5)=C1)[O-].O=C(O)C(F)(F)F
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Synonyms
JF549 TFA
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
용액&용해도
In Vitro:
DMSO : 25 mg/mL (43.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7590 mL | 8.7951 mL | 17.5901 mL | 43.9754 mL |
| 5 mM | 0.3518 mL | 1.7590 mL | 3.5180 mL | 8.7951 mL | |
| 10 mM | 0.1759 mL | 0.8795 mL | 1.7590 mL | 4.3975 mL | |
| 15 mM | 0.1173 mL | 0.5863 mL | 1.1727 mL | 2.9317 mL | |
| 20 mM | 0.0880 mL | 0.4398 mL | 0.8795 mL | 2.1988 mL | |
| 25 mM | 0.0704 mL | 0.3518 mL | 0.7036 mL | 1.7590 mL | |
| 30 mM | 0.0586 mL | 0.2932 mL | 0.5863 mL | 1.4658 mL | |
| 40 mM | 0.0440 mL | 0.2199 mL | 0.4398 mL | 1.0994 mL |
Janelia Fluor® 549 TFA Related Classifications
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