2-Thiouridine
Based on 1 Customer Validation
2-Thiouridine is an orally active modified nucleobase. 2-Thiouridine stabilizes U:A base pairs and destabilizes U:G wobble base pairs. 2-Thiouridine significantly improves the efficiency and accuracy of nonenzymatic replication of mixed-sequence A/U-containing RNA templates. 2-Thiouridine exhibits antiviral activity against multiple positive-sense single-stranded RNA viruses (DENV2, ZIKV, YFV, JEV, WNV, CHIKV, human coronaviruses ( [HCoV]-229E, HCoV-OC43, SARS-CoV), and MERS-CoV).
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 20235-78-3
- Formula: C9H12N2O5S
- Molecular Weight:260.27
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
2-Thiouridine (0.625-40 μM) inhibits the replication of ssRNA+ viruses (DENV2, ZIKV, YFV, JEV, WNV, CHIKV, human coronaviruses ([HCoV]-229E, HCoV-OC43, SARS-CoV), and MERS-CoV) in a dose-dependent manner[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
2-Thiouridine (2-300 mg/kg; i.v. (2-20 mg/kg; once a day) or p.o. (300 mg/kg; twice a day); 5 days) improves the survival rate and reduces the lung viral titer and inflammatory factor levels in the SARS-CoV-2 infection mouse model[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 20235-78-3
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Appearance Solid
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Molecular Weight 260.27
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Formula C9H12N2O5S
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Color White to light yellow
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SMILES
S=C(N1)N([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)C=CC1=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (192.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 10 mg/mL (38.42 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Heuberger BD, et al. Replacing uridine with 2-thiouridine enhances the rate and fidelity of nonenzymatic RNA primer extension. J Am Chem Soc. 2015 Feb 25;137(7):2769-75. [Content Brief]
[2]. Kumar RK, et al. Synthesis and studies on the effect of 2-thiouridine and 4-thiouridine on sugar conformation and RNA duplex stability. Nucleic Acids Res. 1997 Mar 15;25(6):1272-80. [Content Brief]
[3]. Larsen AT, et al. Thermodynamic insights into 2-thiouridine-enhanced RNA hybridization. Nucleic Acids Res. 2015 Sep 18;43(16):7675-87. [Content Brief]
[4]. Uemura K, et al. 2-thiouridine is a broad-spectrum antiviral nucleoside analogue against positive-strand RNA viruses. Proc Natl Acad Sci U S A. 2023 Oct 17;120(42):e2304139120. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.8422 mL | 19.2108 mL | 38.4216 mL | 96.0541 mL |
| 5 mM | 0.7684 mL | 3.8422 mL | 7.6843 mL | 19.2108 mL | |
| 10 mM | 0.3842 mL | 1.9211 mL | 3.8422 mL | 9.6054 mL | |
| 15 mM | 0.2561 mL | 1.2807 mL | 2.5614 mL | 6.4036 mL | |
| 20 mM | 0.1921 mL | 0.9605 mL | 1.9211 mL | 4.8027 mL | |
| 25 mM | 0.1537 mL | 0.7684 mL | 1.5369 mL | 3.8422 mL | |
| 30 mM | 0.1281 mL | 0.6404 mL | 1.2807 mL | 3.2018 mL | |
| DMSO | 40 mM | 0.0961 mL | 0.4803 mL | 0.9605 mL | 2.4014 mL |
| 50 mM | 0.0768 mL | 0.3842 mL | 0.7684 mL | 1.9211 mL | |
| 60 mM | 0.0640 mL | 0.3202 mL | 0.6404 mL | 1.6009 mL | |
| 80 mM | 0.0480 mL | 0.2401 mL | 0.4803 mL | 1.2007 mL | |
| 100 mM | 0.0384 mL | 0.1921 mL | 0.3842 mL | 0.9605 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
2-Thiouridine Related Classifications
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