Fluorescein
Based on 14 publication(s) in Google Scholar
Fluorescein (Uranine) is widely used as a fluorescent tracer in medicinal and biological applications and tumor infected tissues tracer. Fluorescein (Uranine) is a representative green fluorophore that has been widely used as a scaffold of practically useful green fluorescent probes.
For research use only. We do not sell to patients.
- Purity: 96.32%
- CAS No.: 2321-07-5
- Formula: C20H12O5
- Molecular Weight:332.31
-
Storage:
RT, protect from light, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Fluorescein
More- Sci Bull. 2024 Mar 15;69(5):621-635. [Abstract]
- Cell Death Dis. 2023 Feb 7;14(2):91. [Abstract]
- Food Chem. 2026 Mar 28:515:149066. [Abstract]
- Food Sci Hum Wellness. 2026.
- J Agric Food Chem. 2025 Jul 16;73(28):17696-17712. [Abstract]
- Commun Biol. 2025 Apr 26;8(1):667. [Abstract]
- Brain Res Bull. 2025 Sep:229:111479. [Abstract]
- J Biol Chem. 2026 May 27:113207. [Abstract]
- J Neurosurg. 2018 Sep 7;131(3):724-734. [Abstract]
- Brain Res. 2024 Jul 15:1835:148919. [Abstract]
- Gene. 2025 Jan 27:149292. [Abstract]
- Dev Dyn. 2025 Jun;254(6):513-537. [Abstract]
- Med Mol Morphol. 2025 Nov 10. [Abstract]
- J Otol. 2025 Nov 13;20(4):211-218. [Abstract]
Biological Activity
525
488
Chemical Information
-
CAS No. 2321-07-5
-
Appearance Solid
-
Molecular Weight 332.31
-
Formula C20H12O5
-
Color Pink to red
-
SMILES
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC=C5
-
Synonyms
Uranine
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
RT, protect from light, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
Publications (14)
-
Journal Impact Factor
-
Most Recent
-
Sci Bull
Neurological impairment is crucial for tire rubber-derived contaminant 6PPDQ-induced acute toxicity to rainbow trout. [Abstract]2024 Mar 15;69(5):621-635. PMID: 38185590 -
Cell Death Dis
RBM3 suppresses stemness remodeling of prostate cancer in bone microenvironment by modulating N6-methyladenosine on CTNNB1 mRNA. [Abstract]2023 Feb 7;14(2):91. PMID: 36750551 -
Food Chem
Optimization of multi-enzyme coupled debittering process: Effect on bitterness and bioactive components of Shatianyu (Citrus grandis L. Osbeck) whole fruit powder. [Abstract]2026 Mar 28:515:149066. PMID: 41990492 -
-
J Agric Food Chem
Tryptophan-Rich Diet Improves High-Fat Diet-Induced Cognitive Dysfunction and Blood-Brain Barrier Disruption in C57BL/6 Mice through FFAR3 Activation. [Abstract]2025 Jul 16;73(28):17696-17712. PMID: 40600293 -
Commun Biol
Nanocrystalline cellulose-geniposide complex enhances gut-brain axis modulation for depression treatment. [Abstract]2025 Apr 26;8(1):667. PMID: 40287572 -
Brain Res Bull
Sevoflurane reduces brain edema and improves blood-brain barrier by downregulating CaMKII to inhibit TMEM16A after cerebral ischemia injury both in vivo and in vitro. [Abstract]2025 Sep:229:111479. PMID: 40714175 -
J Biol Chem
Lactate-fueled H3K18 lactylation sustains ΔNp63α-dependent self-renewal in limbal epithelial stem cells. [Abstract]2026 May 27:113207. PMID: 42208899 -
J Neurosurg
Elucidating the kinetics of sodium fluorescein for fluorescence-guided surgery of glioma. [Abstract]2018 Sep 7;131(3):724-734. PMID: 30192200 -
Brain Res
2024 Jul 15:1835:148919. PMID: 38588846 -
Gene
Developmental endothelial locus-1 as a potential biomarker for the formation and progression of intracranial aneurysm. [Abstract]2025 Jan 27:149292. PMID: 39880340 -
Dev Dyn
Spatiotemporal characteristics of eustachian tube development in C57BL/6 mice: Correlation between morphological and functional maturation. [Abstract]2025 Jun;254(6):513-537. PMID: 39422348 -
Med Mol Morphol
THBS1 inhibition alleviates inflammatory response by inhibiting TGF-β and NLRP3 inflammasome in experimental murine dry eye. [Abstract]2025 Nov 10. PMID: 41212269 -
J Otol
2025 Nov 13;20(4):211-218. PMID: 41311539
Solvent & Solubility
DMSO : 83.33 mg/mL (250.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Nabel A Negm, et al. Fluorescein dye derivatives and their nanohybrids: Synthesis, characterization and antimicrobial activity. J Photochem Photobiol B. 2016 Sep;162:421-433. [Content Brief]
[2]. Hirabayashi K, et al. Analysis of chemical equilibrium of silicon-substituted fluorescein and its application to develop a scaffold for red fluorescent probes. Anal Chem. 2015;87(17):9061-9069. [Content Brief]
[3]. Li Liu, et al. Fluorescein as an artificial enzyme to mimic peroxidase. Chem Commun (Camb). 2016 Nov24;52(96):13912-13915. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0092 mL | 15.0462 mL | 30.0924 mL | 75.2310 mL |
| 5 mM | 0.6018 mL | 3.0092 mL | 6.0185 mL | 15.0462 mL | |
| 10 mM | 0.3009 mL | 1.5046 mL | 3.0092 mL | 7.5231 mL | |
| 15 mM | 0.2006 mL | 1.0031 mL | 2.0062 mL | 5.0154 mL | |
| 20 mM | 0.1505 mL | 0.7523 mL | 1.5046 mL | 3.7615 mL | |
| 25 mM | 0.1204 mL | 0.6018 mL | 1.2037 mL | 3.0092 mL | |
| 30 mM | 0.1003 mL | 0.5015 mL | 1.0031 mL | 2.5077 mL | |
| 40 mM | 0.0752 mL | 0.3762 mL | 0.7523 mL | 1.8808 mL | |
| 50 mM | 0.0602 mL | 0.3009 mL | 0.6018 mL | 1.5046 mL | |
| 60 mM | 0.0502 mL | 0.2508 mL | 0.5015 mL | 1.2538 mL | |
| 80 mM | 0.0376 mL | 0.1881 mL | 0.3762 mL | 0.9404 mL | |
| 100 mM | 0.0301 mL | 0.1505 mL | 0.3009 mL | 0.7523 mL |
Fluorescein Related Classifications
HY-D0251 Related Classifications
Powered by Bioz