UZH2
Based on 1 publication(s) in Google Scholar
UZH2 is a potent and selective METTL3 inhibitor with an IC50 value of 5 nM.
For research use only. We do not sell to patients.
- Purity: 98.91%
- CAS No.: 2756566-45-5
- Formula: C27H37F2N7O
- Molecular Weight:513.63
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) UZH2
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Biological Activity
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METTL3-14 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MOLM-13 | EC50 |
0.7 μM
Compound: 22; UZH2
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Reduction in m6A/A ratio in poly-adenylated RNA fraction of human MOLM-13 cells measured after 16 hrs by UPLC-MS/MS analysis
Reduction in m6A/A ratio in poly-adenylated RNA fraction of human MOLM-13 cells measured after 16 hrs by UPLC-MS/MS analysis
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[PMID: 34431664] |
| MOLM-13 | GI50 |
12 μM
Compound: 22; UZH2
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Growth inhibition of human MOLM-13 cells assessed as cell viability measured after 3 days
Growth inhibition of human MOLM-13 cells assessed as cell viability measured after 3 days
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[PMID: 34431664] |
| PC-3 | EC50 |
2.5 μM
Compound: 22; UZH2
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Reduction in m6A/A ratio in poly-adenylated RNA fraction of human PC-3 cells measured after 16 hrs by UPLC-MS/MS analysis
Reduction in m6A/A ratio in poly-adenylated RNA fraction of human PC-3 cells measured after 16 hrs by UPLC-MS/MS analysis
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[PMID: 34431664] |
| PC-3 | GI50 |
70 μM
Compound: 22; UZH2
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Growth inhibition of human PC-3 cells assessed as cell viability measured after 3 days
Growth inhibition of human PC-3 cells assessed as cell viability measured after 3 days
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[PMID: 34431664] |
UZH2 (compound 22) (0-25 μM, 1 h) stabilizes METTL3 in a dosedependent manner at 54 °C， with an EC50 of 0.85 μM in MOLM-13 cells[1].
UZH2 (16 h) reduces m6A/A ratio in polyadenylated RNA in MOLM-13 and PC-3 cells，with the EC50 of 0.7 and 2.5 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MOLM-13 cells
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Concentration:0-25 μM
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Incubation Time:1h
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Result:Stabilized METTL3 in a dosedependent manner at 54 °C.
Chemical Information
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CAS No. 2756566-45-5
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Appearance Solid
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Molecular Weight 513.63
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Formula C27H37F2N7O
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Color White to off-white
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SMILES
CNC1=NC=NC(N2CCC3(CN(CC(N3)=O)C4=C(C=C(C(F)=C4)CN5CCC(C)(CC5)C)F)CC2)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 50 mg/mL (97.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.43 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9469 mL | 9.7346 mL | 19.4693 mL | 48.6732 mL |
| 5 mM | 0.3894 mL | 1.9469 mL | 3.8939 mL | 9.7346 mL | |
| 10 mM | 0.1947 mL | 0.9735 mL | 1.9469 mL | 4.8673 mL | |
| 15 mM | 0.1298 mL | 0.6490 mL | 1.2980 mL | 3.2449 mL | |
| 20 mM | 0.0973 mL | 0.4867 mL | 0.9735 mL | 2.4337 mL | |
| 25 mM | 0.0779 mL | 0.3894 mL | 0.7788 mL | 1.9469 mL | |
| 30 mM | 0.0649 mL | 0.3245 mL | 0.6490 mL | 1.6224 mL | |
| 40 mM | 0.0487 mL | 0.2434 mL | 0.4867 mL | 1.2168 mL | |
| 50 mM | 0.0389 mL | 0.1947 mL | 0.3894 mL | 0.9735 mL | |
| 60 mM | 0.0324 mL | 0.1622 mL | 0.3245 mL | 0.8112 mL | |
| 80 mM | 0.0243 mL | 0.1217 mL | 0.2434 mL | 0.6084 mL |
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