CU-CPT17e
Based on 3 publication(s) in Google Scholar
CU-CPT17e is a potent multi-Toll-like receptor (TLR) agonist that activates TLR3, TLR8, and TLR9.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.67%
- CAS 番号: 2109805-75-4
- 分子式: C27H24N2O8
- 分子量:504.49
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 CU-CPT17e
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生物活性
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TLR3 |
TLR8 |
TLR9 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
>100 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR2 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR2 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
>100 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR4 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR4 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
>100 μM
Compound: 17e; CU-CPT17e
|
Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
>100 μM
Compound: 17e; CU-CPT17e
|
Agonist activity at human TLR7 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR7 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
13.45 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR8 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR8 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
4.8 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR3 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR3 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
|
[PMID: 28537730] |
| HEK293 | EC50 |
5.66 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR9 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR9 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HeLa | IC50 |
15.77 μM
Compound: 17e; CU-CPT17e
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Antiproliferative activity against human HeLa cells after 48 hrs by WST1 assay
Antiproliferative activity against human HeLa cells after 48 hrs by WST1 assay
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[PMID: 28537730] |
| HeLa | IC50 |
2.71 μM
Compound: 17e; CU-CPT17e
|
Antiproliferative activity against human HeLa cells after 72 hrs by WST1 assay
Antiproliferative activity against human HeLa cells after 72 hrs by WST1 assay
|
[PMID: 28537730] |
| HeLa | IC50 |
38.56 μM
Compound: 17e; CU-CPT17e
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Antiproliferative activity against human HeLa cells after 24 hrs by WST1 assay
Antiproliferative activity against human HeLa cells after 24 hrs by WST1 assay
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[PMID: 28537730] |
| HMEC | IC50 |
>100 μM
Compound: 17e; CU-CPT17e
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Antiproliferative activity against HMEC after 24 hrs by WST1 assay
Antiproliferative activity against HMEC after 24 hrs by WST1 assay
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[PMID: 28537730] |
| HMEC | IC50 |
>100 μM
Compound: 17e; CU-CPT17e
|
Antiproliferative activity against HMEC after 48 hrs by WST1 assay
Antiproliferative activity against HMEC after 48 hrs by WST1 assay
|
[PMID: 28537730] |
| HMEC | IC50 |
79.53 μM
Compound: 17e; CU-CPT17e
|
Antiproliferative activity against HMEC after 72 hrs by WST1 assay
Antiproliferative activity against HMEC after 72 hrs by WST1 assay
|
[PMID: 28537730] |
CU-CPT17e shows strong NF-κB activation in TLR3, TLR8 and TLR9 HEK293 cells with EC50 values of 4.80±0.73, 13.5±0.58 and 5.66±0.17 μM, respectively. CU-CPT17e significantly improves the activity with 13.9±0.9 fold of NF-κB activation and an EC50 value of 4.8±0.7 μM. CU-CPT17e inhibits the proliferation of HeLa cancer cells by triggering apoptosis and arresting the cell cycle at the S phase. The induction of apoptosis by CU-CPT17e in HeLa cells is investigated. HeLa cells are cultured with increasing concentrations of CU-CPT17e or poly I:C or blank control (DMSO) for 24 h. Treatment with CU-CPT17e for 24 h at different concentrations (10 to 40 μM) results in an elevation of apoptotic cell population ranging from 10% to 17%, which is more effective than poly I:C at 5 μg/mL. These results suggest that the antiproliferative activity of CU-CPT17e against HeLa cells might result from its ability to directly induce apoptosis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 2109805-75-4
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性状 Solid
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分子量 504.49
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分子式 C27H24N2O8
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Color Light yellow to yellow
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SMILES
O=[N+]([O-])C(C=C1)=CC=C1COC2=CC(C=CC3(CCOCC3)O4)=C4C=C2OCC5=CC=C([N+]([O-])=O)C=C5
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Wound Repair Regen
Foamy macrophages potentially inhibit tuberculous wound healing by inhibiting the TLRs/NF-κB signalling pathway. [Abstract]2022 May;30(3):376-396. PMID: 35384137 -
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溶剤 & 溶解度
DMSO : 5 mg/mL (9.91 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
プロトコル
HeLa cells are seeded at a density of 3×105 cells/well in 6-well plates and allowed to attach for 24 h. After treatment of indicated concentrations of CU-CPT17e or poly I:C (5 μg/mL) for another 24 h, cells are harvested with 0.25% trypsin without EDTA and rinsed twice with PBS, then stained using a Annexin V-FITC apoptosis detection kit. Cells are analyzed with a BD Accuri C6 flow cytometer[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
純度とドキュメンテーション
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データシート (276 KB)
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SDS (544 KB)
- English - EN (544 KB)
- Français - FR (544 KB)
- Deutsch - DE (544 KB)
- Norwegian - NO (544 KB)
- Español - ES (544 KB)
- Swedish - SV (544 KB)
- Italian - IT (544 KB)
- Korean - KR (544 KB)
- Portuguese - PT (544 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9822 mL | 9.9110 mL | 19.8220 mL | 49.5550 mL |
| 5 mM | 0.3964 mL | 1.9822 mL | 3.9644 mL | 9.9110 mL |
CU-CPT17e 関連分類
HY-101929 関連分類
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