Gboxin
Based on 4 publication(s) in Google Scholar
Gboxin is an oxidative phosphorylation (OXPHOS) inhibitor that targets glioblastoma. Gboxin inhibits the activity of F0F1 ATP synthase. Antitumour activity.
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- 純度: 99.03%
- CAS 番号: 2101315-36-8
- 分子式: C22H33ClN2O2
- 分子量:392.96
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保管条件:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
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In Vivo Efficacy Study
生物活性
F0F1 ATP synthase[1]
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Cell Line
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Type | Value | Description | References |
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| GBM | IC50 |
1 μM
Compound: Gboxin
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Growth inhibition of patient derived GBM cells incubated for 4 days by Celltiter glo assay
Growth inhibition of patient derived GBM cells incubated for 4 days by Celltiter glo assay
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[PMID: 33103432] |
| GBM | IC50 |
150 nM
Compound: Gboxin
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Anticancer activity against human GBM cells carrying high-throughput GBM sphere assessed as reduction in cell viability incubated for 96 hrs by Cell-Titer-Glo assay
Anticancer activity against human GBM cells carrying high-throughput GBM sphere assessed as reduction in cell viability incubated for 96 hrs by Cell-Titer-Glo assay
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[PMID: 32942072] |
Gboxin (0-15 μM; 96 hours) specifically inhibit the growth of primary ‘high-throughput GBM sphere’ (HTS) cells but not that of cycling primary low-passage mouse embryonic fibroblasts (MEFs) or astrocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HTS cells, MEFs and astrocytes
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Concentration:0, 185, 555, 1667, 5000, 15000 nM
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Incubation Time:96 hours
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Result:Inhibited the growth of HTS cells (IC50=150 nM).
化学情報
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CAS 番号 2101315-36-8
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性状 Solid
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分子量 392.96
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分子式 C22H33ClN2O2
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Color White to off-white
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SMILES
O=C(O[C@H]1[C@H](C(C)C)CC[C@@H](C)C1)CN2C(CC)=[N+](C)C3=CC=CC=C23.[Cl-]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (4)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Tumor cell-intrinsic epigenetic dysregulation shapes cancer-associated fibroblasts heterogeneity to metabolically support pancreatic cancer. [Abstract]2024 May 13;42(5):869-884.e9. PMID: 38579725 -
Nat Commun
Cancer cell-mitochondria hybrid membrane coated Gboxin loaded nanomedicines for glioblastoma treatment. [Abstract]2023 Jul 28;14(1):4557. PMID: 37507371
Gboxin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jul 28;14(1):4557. [Abstract]
U87MG cell viability after 72 h incubation with free Gboxin, NPs@G, CM-NPs@G, MM-NPs@G, or HM-NPs@G (n = 4 biologically independent samples).
Gboxin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jul 28;14(1):4557. [Abstract]
IC50 values in normal cells (N2a, BV2, HA1800 and hCMEC/D3 cells) and GBM cells (U87MG, U251, U251-TR and X01) after 72 h incubation with HM-NPs@G or free Gboxin.
Gboxin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jul 28;14(1):4557. [Abstract]
Pharmacokinetic profiles of HM-NPs@G, CM-NPs@G, MM-NPs@G or NPs@G in healthy BALB/c mice with free Gboxin as control (5 mg Gboxin equiv. kg−1, n = 3 mice in each group).
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ACS Appl Mater Interfaces
Peptide Hydrogels for Renal Carcinoma Therapy via Synergistic Inhibition of Glycolysis and Mitochondrial Metabolism Reprogramming. [Abstract]2025 Jun 25;17(25):36487-36496. PMID: 40518708 -
Biochem Pharmacol
ONC212 enhances YM155 cytotoxicity by triggering SLC35F2 expression and NOXA-dependent MCL1 degradation in acute myeloid leukemia cells. [Abstract]2024 Jun:224:116242. PMID: 38679209
Gboxin purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2024 Jun:224:116242. [Abstract]
Effect of gboxin on the viability of U937 and U937/HQ cells. Cells were treated with indicated gboxin (0-30 μM) concentrations for 24 h. Cell viability was determined using an MTT assay.
溶剤 & 溶解度
DMSO : 32.5 mg/mL (82.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 10 mg/mL (25.45 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.17 mg/mL (5.52 mM); Clear solution
This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.17 mg/mL (5.52 mM); Clear solution
This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 2 mg/mL (5.09 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.5448 mL | 12.7239 mL | 25.4479 mL | 63.6197 mL |
| 5 mM | 0.5090 mL | 2.5448 mL | 5.0896 mL | 12.7239 mL | |
| 10 mM | 0.2545 mL | 1.2724 mL | 2.5448 mL | 6.3620 mL | |
| 15 mM | 0.1697 mL | 0.8483 mL | 1.6965 mL | 4.2413 mL | |
| 20 mM | 0.1272 mL | 0.6362 mL | 1.2724 mL | 3.1810 mL | |
| 25 mM | 0.1018 mL | 0.5090 mL | 1.0179 mL | 2.5448 mL | |
| DMSO | 30 mM | 0.0848 mL | 0.4241 mL | 0.8483 mL | 2.1207 mL |
| 40 mM | 0.0636 mL | 0.3181 mL | 0.6362 mL | 1.5905 mL | |
| 50 mM | 0.0509 mL | 0.2545 mL | 0.5090 mL | 1.2724 mL | |
| 60 mM | 0.0424 mL | 0.2121 mL | 0.4241 mL | 1.0603 mL | |
| 80 mM | 0.0318 mL | 0.1590 mL | 0.3181 mL | 0.7952 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Gboxin 関連分類
HY-111651 関連分類
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