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AFM24

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Based on 1 Customer Validation

AFM24 is a bispecific antibody with a TandAb structure expressed in CHO, targeting EGFR&Fc-gamma-RIIIA. AFM24 contains a huIgG1-SCFV heavy chain and a λ light chain, with a predicted molecular weight (MW) of 196.34 kDa. The isotype control for AFM24 can refer to Human IgG1 kappa, Isotype Control (HY-P99001).

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  • 純度: 96.26%
  • 分子量:196.34 kDa
  • 保管条件:

    Please store the product under the recommended conditions in the Certificate of Analysis.

  • Experimental Validation Results for AFM24
  • Experimental Validation Results for AFM24
  • Experimental Validation Results for AFM24
  • Experimental Validation Results for AFM24
  • Experimental Validation Results for AFM24
  • AFM24 Chemical Structure
    AFM24 構造式
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
最寄りの代理店をお探しください Your local distributor: Hycultec GmbHHycultec
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Assay Types
  • 1/16

    tetravalent bispecific IgG1-scFv fusion antibody (scFv-IgAb)

  • 1/16

    Immobilized Fc gamma RIIA/CD32a Protein, Human can bind AFM24. The EC50 for this effect is 93.45 ng/mL.

  • 1/16

    Immobilized EGFR Protein, Human can bind AFM24. The EC50 for this effect is 86.89 ng/mL.

  • 1/16

    Flow Cytometry analysis of Hela cells labelling EGFR (red) with AFM24 (HY-P990314). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    Flow cytometric analysis of 1X106 Jurkat cells labeling Fc-gamma-RIIIA with AFM24 (HY-P990314, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 for an hour at 4℃. AF488-conjugated Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control.

  • AFM24 Chemical Structure
    1/16
  • AFM24
  • Immobilized Fc gamma RIIA/CD32a Protein, Human can bind AFM24. The EC50 for this effect is 93.45 ng/mL.
  • Immobilized EGFR Protein, Human can bind AFM24. The EC50 for this effect is 86.89 ng/mL.
  • Flow Cytometry analysis of Hela cells labelling EGFR (red) with AFM24 (HY-P990314). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Flow cytometric analysis of 1X106 Jurkat cells labeling Fc-gamma-RIIIA with AFM24 (HY-P990314, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 for an hour at 4℃. AF488-conjugated Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control.
  • tetravalent bispecific IgG1-scFv fusion antibody (scFv-IgAb)