Anserinone B
Anserinone B is an antifungal and antibacterial benzoquinone. Anserinone B causes radial growth reductions of 50% and 37% against S.fimicola and A. furfuraceus, respectively. Anserinones B also displays moderate cytotoxicity in the NCI’s 60 human tumor cell line panel (GI50=4.4 µg/mL).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 190895-96-6
- 分子式: C11H14O4
- 分子量:210.23
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
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Help & FAQs
Endogenous Metabolite アイソフォーム固有の製品をすべて表示
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生物活性
製品説明
IC50 & Target
[1]|
Microbial Metabolite |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| C6 | IC50 |
<10 μM
Compound: 8
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Cytotoxicity against rat C6 cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
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[PMID: 34787427] |
| HGC-27 | IC50 |
<10 μM
Compound: 8
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Cytotoxicity against human HGC-27 cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
Cytotoxicity against human HGC-27 cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
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[PMID: 34787427] |
| MDA-MB-435 | IC50 |
3.6 μg/mL
Compound: 2
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Cytotoxicity against human MDA-MB-435 after 48 hrs by SRB assay
Cytotoxicity against human MDA-MB-435 after 48 hrs by SRB assay
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[PMID: 15043411] |
| SH-SY5Y | IC50 |
<10 μM
Compound: 8
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Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
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[PMID: 34787427] |
化学情報
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CAS 番号 190895-96-6
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分子量 210.23
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分子式 C11H14O4
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SMILES
O=C(C=C1OC)C(C)=C(C[C@H](O)C)C1=O
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
純度とドキュメンテーション
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Anserinone B 関連分類
HY-N10307 関連分類
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