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製品番号: HY-146038

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Antitumor agent-55

Antitumor agent-55 (compound 5q) is a potent antitumor agent. Antitumor agent-55 effectively inhibits PC3, with an IC₅₀ of 0.91 μM. Antitumor agent-55 effectively inhibits the colony formation, suppresses the cell migration in PC3. Antitumor agent-55 induces G1/S phase arrest and apoptosis in PC3.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 2522594-49-4
分子式: C₃₂H₃₄N₆O₄S
分子量:598.72

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (279 KB)

Antitumor agent-55 構造式

CAS 番号: 2522594-49-4

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

Bcl-2 Family アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	11.54 μM Compound: 5q	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 24 to 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 24 to 72 hrs by MTT assay	[PMID: 32619887]
MGC-803	IC₅₀	8.21 μM Compound: 5q	Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 to 72 hrs by MTT assay Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 to 72 hrs by MTT assay	[PMID: 32619887]
PC-3	IC₅₀	0.91 μM Compound: 5q	Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 24 to 72 hrs by MTT assay Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 24 to 72 hrs by MTT assay	[PMID: 32619887]
PC-9	IC₅₀	34.68 μM Compound: 5q	Antiproliferative activity against human PC9 cells assessed as reduction in cell viability after 24 to 72 hrs by MTT assay Antiproliferative activity against human PC9 cells assessed as reduction in cell viability after 24 to 72 hrs by MTT assay	[PMID: 32619887]

体外実験

Antitumor agent-55 (compound 5q) shows inhibitory activity against MCF-7, PC3, MGC-803, PC9, and WPMY-1 (normal human prostatic stromal myofibroblast cell line), with IC₅₀ values of 11.54 ± 0.18, 0.91 ± 0.31, 8.21 ± 0.50, 34.68 ± 0.67, and 48.15 ± 0.33, respectively^[1].
Antitumor agent-55 (0-10 μM, 24-72 h) significantly inhibits the proliferation of PC3 cells dose- and time-dependently^[1].
Antitumor agent-55 (0-4 μM, 24 h) increases the G1/S phase population, and dose-dependently elevates the expression of p27 protein^[1].
Antitumor agent-55 (0-4 μM, 24-48 h) dose-dependently induces the accumulation of ROS, and induces apoptosis of PC3 cells through activating the two apoptotic signaling pathways simultaneously^[1].
Antitumor agent-55 (0-1 μM, 48 h) effectively inhibits the wound healing and the migration of PC3 cells in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:

PC3 cells^[1]
Concentration:

0, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10 μM
Incubation Time:

24, 48, 72 h
Result:

Significantly inhibited the proliferation of PC3 cells dose- and time-dependently, formed fewer and smaller colonies.

Cell Cycle Analysis

Cell Line:

PC3 cells^[1]
Concentration:

0, 1, 2, 4 μM
Incubation Time:

24 h
Result:

Significantly increased the G1/S phase population while decreased G2/M content at high concentration in PC3 cells.

Western Blot Analysis

Cell Line:

PC3 cells^[1]
Concentration:

0, 1, 2, 4 μM
Incubation Time:

24 h， 48 h
Result:

Dose-dependently elevated the expression of p27 protein, markedly elevated the expression of pro-apoptotic Bax and P53 while anti-apoptotic Bcl-2 expression was down-regulated, and significantly increased the expression of cleaved caspase 3/9 and cleaved PARP in a dose-dependent manner.

Apoptosis Analysis

Cell Line:

PC3 cells^[1]
Concentration:

0, 1, 2, 4 μM
Incubation Time:

48 h
Result:

Dose-dependently led to significant increase of apoptotic population, and the apoptotic percentage was up to 70.7% at 4 μM, which was far higher than the control group (3.5%).

Related Protocols :

化学情報

CAS 番号 2522594-49-4
分子量 598.72
分子式 C₃₂H₃₄N₆O₄S
SMILES

O=C(NC1CN(C2=CC(C)=NC3=NC(SCC4=C5C=CC=CC5=CC=C4)=NN23)CCC1)C6=CC(OC)=C(OC)C(OC)=C6
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (279 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Lu N, Huo JL, Wang S, Yuan XH, Liu HM. Drug repurposing: Discovery of troxipide analogs as potent antitumor agents. Eur J Med Chem. 2020;202:112471. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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分子量分子量

希釈計算機

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濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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