dNaMTP
dNaMTP is an unnatural deoxyribonucleoside triphosphate substrate that DNA polymerases use to replicate unnatural base pairs containing dNaM. dNaMTP enables replication, transcription, and translation of DNA with an expanded genetic alphabet in semi-synthetic organisms. dNaMTP is used in synthetic biology research such as unnatural base pair replication analysis, preparation of DNA with an expanded genetic alphabet, and general sequencing of DNA containing unnatural bases.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 1117893-11-4
- 分子式: C16H21O13P3
- 分子量:514.25
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示
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生物活性
製品説明
体外実験
dNaMTP supports the amplification and assembly of pINF containing dNaM paired opposite dTPT3 in cell-free PCR[2].
dNaMTP can participate in gap insertion, labeled ligation, and PCR amplification reactions of KRAS-related DNA substrates under specific conditions, and some unnatural base sites cause premature termination in Sanger sequencing[3].
dNaMTP can be used together with dTPT3TP for PCR amplification to prepare IL-2 and pylT gene fragments containing the XY unnatural base pair, which are then assembled via Golden Gate to obtain an expression plasmid carrying this unnatural base pair[4].
dNaMTP and dTPT3TP are added together to the culture medium to maintain the stability of the XY unnatural base pair in E. coli during electroporation-based construction of the AzK-substituted IL-2 expression strain[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 1117893-11-4
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分子量 514.25
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分子式 C16H21O13P3
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SMILES
OP(OP(OP(O)(OC[C@@H]1[C@@H](O)C[C@H](C2=CC3=C(C=CC=C3)C=C2OC)O1)=O)(O)=O)(O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Wu J, et al. Engineered polymerase-mediated efficient synthesis of site-specifically functionalized RNA and 2’-modified RNA oligonucleotides via genetic alphabet expansion. Nature Communications. 2026 Aug 13. [Content Brief]
[2]. Malyshev DA, et al. A semi-synthetic organism with an expanded genetic alphabet. Nature. 2014 May 15;509(7500):385-8. [Content Brief]
[3]. Riedl J, et al. Identification of DNA lesions using a third base pair for amplification and nanopore sequencing. Nature communications. 2015 Nov 06;6:8807. [Content Brief]
[4]. Ptacin JL, et al. An engineered IL-2 reprogrammed for anti-tumor therapy using a semi-synthetic organism. Nature communications. 2021 Aug 09;12(1):4785. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)