eCF309
Based on 1 Customer Validation
eCF309 is a potent and highly selective mTOR inhibitor with an IC50 of 15 nM. eCF309 displays higher selectivity over PI3Ks. eCF309 can be used for the study of breast cancer and prostate cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.2%
- CAS 番号: 2001571-40-8
- 分子式: C18H21N7O3
- 分子量:383.40
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保管条件:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | EC50 |
8 nM
Compound: 12
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Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis
Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis
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10.1039/C5MD00493D |
| MDA-MB-231 | EC50 |
72 nM
Compound: 12
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Cytotoxicity against human MDA-MB-231 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
Cytotoxicity against human MDA-MB-231 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
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10.1039/C5MD00493D |
| PC-3 | EC50 |
37 nM
Compound: 12
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Cytotoxicity against human PC3 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
Cytotoxicity against human PC3 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
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10.1039/C5MD00493D |
| T98 | EC50 |
100 nM
Compound: eCF309
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Antiproliferative activity against human T98 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
Antiproliferative activity against human T98 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
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[PMID: 31787462] |
| U-87MG ATCC | EC50 |
100 nM
Compound: eCF309
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Antiproliferative activity against human U87 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
Antiproliferative activity against human U87 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
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[PMID: 31787462] |
eCF309 (0.3-1000 nM; 5 days) exhibits highly potent antiproliferative activity against MCF7, MDA-MB-231 and PC3 cells[1].
eCF309 (0.3-1000 nM; 24 h) causes G0/G1 cell cycle arrest[1].
eCF309 (3-100 nM; 1.5 h) inhibits pP70S6K, pS6 and pAKT levels in MCF7 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF7, MDA-MB-231 and PC3 cells
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Concentration:0.3-1000 nM
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Incubation Time:5 days
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Result:Exhibits EC50 values upon MCF7, MDA-MB-231 and PC3 cells viability of 8.4 nM, 72 nM and 37 nM, respectively.
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Cell Line:MCF7, MDA-MB-231 and PC3 cells
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Concentration:0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM, 300 nM, 1000 nM
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Incubation Time:24 h
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Result:Caused G0/G1 cell cycle arrest.
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Cell Line:MCF7 cells
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Concentration:3 nM, 10 nM, 30 nM, 100 nM
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Incubation Time:For 30 min, followed by 1 h of serum stimulation
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Result:Mediated a dose-dependent reduction of the pP70S6K, pS6 and pAKT levels in MCF7 cells, achieving almost complete inhibition of the phosphorylation of these targets at 30 nM.
化学情報
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CAS 番号 2001571-40-8
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性状 Solid
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分子量 383.40
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分子式 C18H21N7O3
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Color White to off-white
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SMILES
NC1=C2C(N(CC(OCC)OCC)N=C2C3=CC=C(OC(N)=N4)C4=C3)=NC=N1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
溶剤 & 溶解度
DMSO : 1 mg/mL (2.61 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6082 mL | 13.0412 mL | 26.0824 mL | 65.2061 mL |
eCF309 関連分類
HY-128027 関連分類
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