Hibifolin
Based on 1 Customer Validation
Hibifolin is a flavonol glycoside that can be isolated from Helicteres isora. Hibifolin is an inhibitor of adenosine deaminase (ADA) (Ki = 49.92 μM). Hibifolin protects neurons against β-amyloid-induced neurotoxicity. Hibifolin possesses a potent protective activity against cell death induced by aggregated Aβ. Hibifolin can abolish Aβ-induced caspase-3 and caspase-7 activation. Hibifolin induces Akt phosphorylation in cortical neurons. Hibifolin is also a natural sortase A (SrtA) inhibitor (IC50 = 31.2 μM) through direct binding to SrtA protein. Hibifolin attenuates the pathogenic behavior of Staphylococcus aureus including adhesion, invasion, and biofilm formation. Hibifolin improves the survival of pneumonia induced by Staphylococcus aureus in mouse model and alleviates pathological damage. Hibifolin shows a synergistic antibacterial effect with Cefotaxime (HY-A0088A).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.70%
- CAS 番号: 55366-56-8
- 分子式: C21H18O14
- 分子量:494.36
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
Ki: 49.92 μM (ADA)[1]
Hibifolin (100 μM) shows about 60% inhibition of the ADA (10 μM) enzymatic activity[1].
Hibifolin (0.5-50 μM, 24 h) reduces the release of LDH induced by Aβ in cortical neurons[2].
Hibifolin (50 μM, 0-120 sec) reduces the amount of Aβ-induced Ca2+ by 70% in cortical neurons[2].
Hibifolin (50 μM) induces the phosphorylation of Akt to approximately 2-fold[2].
Hibifolin (0-1000 μg/mL) remarkably inhibits the activity of SrtA with an IC50 of 31.2 μM[3].
Hibifolin (32-256 μg/mL) decreases the adhesion of S. fibrinogen to fribinogen with the concentration of 256 μg/mL and significantly inhibits bacterial invasion of A549 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Cortical neurons
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Concentration:0.5, 5, 50 μM
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Incubation Time:24 h
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Result:Had protective effect by preventing Aβ-induced cell death dose-dependently.
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Cell Line:Cortical neurons
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Concentration:50 μM
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Incubation Time:24 h
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Result:Blocked the DNA fragmentation triggered by Aβ.
化学情報
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CAS 番号 55366-56-8
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性状 Solid
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分子量 494.36
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分子式 C21H18O14
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Color Yellow to orange
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SMILES
O[C@H]([C@H]([C@@H]([C@@H](C(O)=O)O1)O)O)[C@@H]1OC2=C3C(C(C(O)=C(C4=CC=C(O)C(O)=C4)O3)=O)=C(O)C=C2O
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
DMSO : 100 mg/mL (202.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.06 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (279 KB)
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SDS (393 KB)
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- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. K G Arun, et al. Inhibitory activity of hibifolin on adenosine deaminase- experimental and molecular modeling study. Comput Biol Chem. 2016 Oct;64:353-358. [Content Brief]
[2]. Judy T T Zhu, et al. Hibifolin, a flavonol glycoside, prevents beta-amyloid-induced neurotoxicity in cultured cortical neurons. Neurosci Lett. 2009 Sep 18;461(2):172-6. [Content Brief]
[3]. Song, W., et al., (2022). Hibifolin, a Natural Sortase A Inhibitor, Attenuates the Pathogenicity of Staphylococcus aureus and Enhances the Antibacterial Activity of Cefotaxime. Microbiology spectrum, 10(4), e0095022. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0228 mL | 10.1141 mL | 20.2282 mL | 50.5704 mL |
| 5 mM | 0.4046 mL | 2.0228 mL | 4.0456 mL | 10.1141 mL | |
| 10 mM | 0.2023 mL | 1.0114 mL | 2.0228 mL | 5.0570 mL | |
| 15 mM | 0.1349 mL | 0.6743 mL | 1.3485 mL | 3.3714 mL | |
| 20 mM | 0.1011 mL | 0.5057 mL | 1.0114 mL | 2.5285 mL | |
| 25 mM | 0.0809 mL | 0.4046 mL | 0.8091 mL | 2.0228 mL | |
| 30 mM | 0.0674 mL | 0.3371 mL | 0.6743 mL | 1.6857 mL | |
| 40 mM | 0.0506 mL | 0.2529 mL | 0.5057 mL | 1.2643 mL | |
| 50 mM | 0.0405 mL | 0.2023 mL | 0.4046 mL | 1.0114 mL | |
| 60 mM | 0.0337 mL | 0.1686 mL | 0.3371 mL | 0.8428 mL | |
| 80 mM | 0.0253 mL | 0.1264 mL | 0.2529 mL | 0.6321 mL | |
| 100 mM | 0.0202 mL | 0.1011 mL | 0.2023 mL | 0.5057 mL |
Hibifolin 関連分類
HY-N7368 関連分類
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