Insulin lispro
Based on 1 Customer Validation
Insulin lispro is a recombinant h-Insulin (HY-P0035) analog whose primary structure is identical to regular h-Insulin, except that the proline-lysine at positions B28 and B29 is translocated to lysine-proline, thereby reducing self-association. Insulin lispro stimulates insulin receptor internalization to early endosomes, induces receptor phosphorylation, intracellular internalization, and colocalization with the early endosome marker EEA1. Insulin lispro shows stronger inhibition of hepatic glucose production and stronger inhibition of glucagon secretion. Insulin lispro has lower mitogenic potency. Insulin lispro can be used in the study of diabetes.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 133107-64-9
- 分子式: C257H383N65O77S6
- 分子量:5807.63
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保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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生物活性
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化学情報
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- 純度とドキュメンテーション
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生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| U2OS | EC50 |
0.0166 nM
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Stimulation of insulin receptor internalization to early endosomes in human osteosarcoma U2OS cells co-expressing hIR isoform B and PLCγ SH2 domain after 2 hrs incubation by PathHunter luminescence assay.
Stimulation of insulin receptor internalization to early endosomes in human osteosarcoma U2OS cells co-expressing hIR isoform B and PLCγ SH2 domain after 2 hrs incubation by PathHunter luminescence assay.
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28576743 |
体外実験
Insulin lispro (5 nM; 5-60 min) induces internalization of phosphorylated IRβ (Tyr1146) in U2OS-hIR cells and colocalizes with early endosomes at 5 to 60 minutes[1].
Insulin lispro (100-≥580 μU/mL) does not cross the placenta at concentrations up to 200 μU/mL, but a small concentration-dependent transfer occurs at concentrations ≥580 μU/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:U2OS cells co-expressing hIR isoform B
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Concentration:5 nM
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Incubation Time:5, 10, 30, 60 min
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Result:Produced a distinct punctate intracellular staining pattern for IRβ compared with control cells.
Showed overlap of EEA1 with the majority of the internalized IRβ signal.
Induced internalized IRβ starting at 5 minutes and throughout the time-course up to 60 minutes.
Showed no overlay of LAMP1 staining with IRβ staining before or after treatment.\nProduced a distinct punctate intracellular staining pattern for phospho-IRβ with no significant immunostaining at the cell surface.
Induced internalized phospho-IRβ starting at 5 minutes and throughout the time-course up to 60 minutes.
Showed overlap of EEA1 with most of the phospho-IRβ signal.
Showed a low level of co-localization of phospho-IRβ with lysosomes.
化学情報
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CAS 番号 133107-64-9
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性状 Solid
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分子量 5807.63
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分子式 C257H383N65O77S6
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Color White to off-white
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SMILES
[Insulin lispro]
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別名
インスリンリスプロ
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
純度とドキュメンテーション
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データシート (270 KB)
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SDS (251 KB)
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- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Insulin lispro 関連分類
HY-109530 関連分類
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