JD-13
JD-13 is a HPV-1 inhibitor and anti-proliferative agent against cancer cells. JD-13 downregulates the expression of HSV-1 DNA polymerase-related genes UL30 and UL42, and inhibits viral replication. JD-13 induces apoptosis (apoptosis) in colorectal cancer cells and suppresses their colony formation. JD-13 can be applied to the research of herpes simplex virus infection and related diseases including colorectal cancer, lung cancer, esophageal cancer and breast cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 2969172-58-3
- 分子式: C25H32N4O3
- 分子量:436.55
-
保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
製品説明
体外実験
JD-13 (0.125-0.5 μM; 72 h) potently inhibits acyclovir-resistant herpes simplex virus type 1 (HSV-1-153) infection in Vero cells, with near-complete inhibition at concentrations of 0.125, 0.25, and 0.5 μM[1].
JD-13 (0.5 μM) most potently inhibits herpes simplex virus type 1 protein expression in Vero cells when administered within the first 6 hours of infection[1].
JD-13 (0.5 μM; 2-6 h) downregulates the expression of herpes simplex virus type 1 DNA polymerase-related genes UL30 and UL42 in Vero cells, with significant inhibition observed at 4 hours for UL42 and 6 hours for both UL30 and UL42[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Vero cells
-
Concentration:0.5 μM
-
Incubation Time:24 h (added at 0 h post-infection); 21 h (added at 3 h post-infection); 18 h (added at 6 h post-infection); 15 h (added at 9 h post-infection); 12 h (added at 12 h post-infection)
-
Result:Showed the strongest inhibitory effect on the expression of HSV-1 proteins gB and ICP0 when added within 6 hours post-infection.
Exhibited weakened inhibitory effect when added at 9 or 12 hours post-infection.
-
Cell Line:Vero cells
-
Concentration:0.5 μM
-
Incubation Time:2 h; 4 h; 6 h
-
Result:Had no significant effect on the expression of UL30 and UL42 at 2 hours post-treatment.
Significantly downregulated UL42 expression at 4 hours post-treatment.
Significantly downregulated both UL30 and UL42 expression at 6 hours post-treatment.
-
Cell Line:HCT116, SW620, A549, Eca109, MDA-MB-231
-
Concentration:Variable concentrations
-
Incubation Time:48 h
-
Result:Inhibited the viability of HCT116, SW620, A549, Eca109, and MDA-MB-231 cells in a concentration-dependent manner.
Had IC50 values of 35.49 nM for HCT116, 24.64 nM for SW620, 36.19 nM for A549, 35.82 nM for Eca109, and 52.14 nM for MDA-MB-231.
Showed significantly lower IC50 values than the reference compound JD-02.
-
Cell Line:HCT116, SW620
-
Concentration:20-80 nM
-
Incubation Time:24 h (followed by 14 days of drug-free culture)
-
Result:Significantly reduced the size and number of cell colonies formed by HCT116 and SW620 cells compared to the control group.
-
Cell Line:HCT116, SW620
-
Concentration:Low, medium, and high doses
-
Incubation Time:48 h
-
Result:Induced typical apoptotic characteristics including cell shrinkage, cytoplasmic condensation, highly condensed and marginalized nuclear chromatin, fragmented nuclei, and apoptotic bodies compared to the blank control group.
Increased the number of cells with strong blue fluorescent apoptotic nuclei in a concentration-dependent manner.
- RNA extraction experimental
- Research Protocol for Infectious Diseases
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Annexin V plus membrane-impermeant dye apoptosis staining
- Apoptosis Solutions
- Soft Agar Colony Formation Assay
- Colony Formation (Clonogenic) Assay
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
化学情報
-
CAS 番号 2969172-58-3
-
分子量 436.55
-
分子式 C25H32N4O3
-
SMILES
O=C(C1=CC=C(N2N=C(C)C3=C2CC(C)(C)CC3=O)C=C1NC45CCC(CC4)(CC5)O)N
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
JD-13 関連分類
HY-184394 関連分類
Powered by Bioz