L-Anserine nitrate
Based on 5 publication(s) in Google Scholar
L-Anserine nitrate is a naturally occurring histidine-containing dipeptide that is found in vertebrate skeletal muscle and animal tissues. L-Anserine nitrate serves as a growth-promoting supplement in serum-free HMI-244 medium for bloodstream forms of Trypanosoma brucei and Trypanosoma evansi. L-Anserine nitrate supports the continuous in vitro proliferation of these parasites, and the proliferated parasites retain their morphological characteristics and infectivity to mice. L-Anserine nitrate can be used in studies of African trypanosomiasis.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.64%
- CAS 番号: 10030-52-1
- 分子式: C10H17N5O6
- 分子量:303.28
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 L-Anserine nitrate
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生物活性
製品説明
体外実験
L-Anserine nitrate (17.0 mg/L; 0-90 days), as part of the serum-free HMI-244 medium, supports continuous axenic growth of bloodstream forms of Trypanosoma brucei IL2343 and Trypanosoma evansi G6 for over 90 days, reaching maximum cell densities of 2.6 × 106 cells/mL and 2.2 × 106 cells/mL with doubling times of 10.6 hours and 10.9 hours, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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CAS 番号 10030-52-1
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性状 Solid
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分子量 303.28
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分子式 C10H17N5O6
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Color White to off-white
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SMILES
O=C(O)[C@@H](NC(CCN)=O)CC1=CN=CN1C.O[N+]([O-])=O
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別名
L-Anserine nitrate
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (5)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
Foods
Quantitative Analysis of Biomarkers to Distinguish Between Korean and Chinese Mud Loaches. [Abstract]2026 Jan 14;15(2):304. PMID: 41596903 -
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Life
Biomarker Development for Identifying Mud Loach (Misgurnus mizolepis) Origin Country Using Untargeted Metabolite Profiling. [Abstract]2023 Oct 31;13(11):2149. PMID: 38004289
溶剤 & 溶解度
体外:
H2O : 250 mg/mL (824.32 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (164.86 mM); Clear solution; Need ultrasonic
プロトコル
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
純度とドキュメンテーション
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データシート (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.2973 mL | 16.4864 mL | 32.9728 mL | 82.4321 mL |
| 5 mM | 0.6595 mL | 3.2973 mL | 6.5946 mL | 16.4864 mL | |
| 10 mM | 0.3297 mL | 1.6486 mL | 3.2973 mL | 8.2432 mL | |
| 15 mM | 0.2198 mL | 1.0991 mL | 2.1982 mL | 5.4955 mL | |
| 20 mM | 0.1649 mL | 0.8243 mL | 1.6486 mL | 4.1216 mL | |
| 25 mM | 0.1319 mL | 0.6595 mL | 1.3189 mL | 3.2973 mL | |
| 30 mM | 0.1099 mL | 0.5495 mL | 1.0991 mL | 2.7477 mL | |
| 40 mM | 0.0824 mL | 0.4122 mL | 0.8243 mL | 2.0608 mL | |
| 50 mM | 0.0659 mL | 0.3297 mL | 0.6595 mL | 1.6486 mL | |
| 60 mM | 0.0550 mL | 0.2748 mL | 0.5495 mL | 1.3739 mL | |
| 80 mM | 0.0412 mL | 0.2061 mL | 0.4122 mL | 1.0304 mL | |
| 100 mM | 0.0330 mL | 0.1649 mL | 0.3297 mL | 0.8243 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Anserine nitrate 関連分類
HY-W011393 関連分類
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