ON1231320
Based on 3 publication(s) in Google Scholar
ON1231320 is a highly specific polo like kinase 2 (PLK2) inhibitor with an IC50 of 0.31 μM. ON1231320 blocks tumor cell cycle progression in the G2/M phase in mitosis, causing apoptotic cell death. ON1231320, an arylsulfonyl pyrido-pyrimidinone, has antitumor activity.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.90%
- CAS 番号: 1312471-39-8
- 分子式: C22H15F2N5O3S
- 分子量:467.45
-
保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
MedChemExpress(MCE)の使用を引用している文献 ON1231320
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生物活性
|
PLK2 0.31 μM (IC50) |
PLK1 >10 μM (IC50) |
PLK3 >10 μM (IC50) |
PLK4 >10 μM (IC50) |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| 5637 | GI50 |
0.125 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human 5637 cells after 72 hrs by trypan blue assay
Growth inhibition of human 5637 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| A-431 | GI50 |
0.03 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human A431 cells after 72 hrs by trypan blue assay
Growth inhibition of human A431 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| A549 | GI50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human A549 cells after 72 hrs by trypan blue assay
Growth inhibition of human A549 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| ASPC1 | GI50 |
0.3 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human AsPC1 cells after 72 hrs by trypan blue assay
Growth inhibition of human AsPC1 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| Bel-7402 | GI50 |
0.05 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human Bel7402 cells after 72 hrs by trypan blue assay
Growth inhibition of human Bel7402 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| BT-474 | GI50 |
0.1 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human BT474 cells after 72 hrs by trypan blue assay
Growth inhibition of human BT474 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| BT-549 | GI50 |
0.25 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human BT549 cells after 72 hrs by trypan blue assay
Growth inhibition of human BT549 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| BXPC-3 | GI50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human BxPC3 cells after 72 hrs by trypan blue assay
Growth inhibition of human BxPC3 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| CAPAN-1 | GI50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human Capan1 cells after 72 hrs by trypan blue assay
Growth inhibition of human Capan1 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| CCRF-CEM | GI50 |
0.05 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human CEM cells after 72 hrs by trypan blue assay
Growth inhibition of human CEM cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| COLO 205 | GI50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human COLO205 cells after 72 hrs by trypan blue assay
Growth inhibition of human COLO205 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| Daudi | GI50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human Daudi cells after 72 hrs by trypan blue assay
Growth inhibition of human Daudi cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| DU-145 | IC50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human DU145 cells after 72 hrs by trypan blue assay
Growth inhibition of human DU145 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| Granta-519 | GI50 |
0.04 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human GRANTA-519 cells after 72 hrs by trypan blue assay
Growth inhibition of human GRANTA-519 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| HCC1806 | GI50 |
0.1 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human HCC1806 cells after 72 hrs by trypan blue assay
Growth inhibition of human HCC1806 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| HCT-116 | GI50 |
0.15 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human HCT116 cells after 72 hrs by trypan blue assay
Growth inhibition of human HCT116 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| HeLa | GI50 |
0.05 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human HeLa cells after 72 hrs by trypan blue assay
Growth inhibition of human HeLa cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| HepG2 | GI50 |
0.5 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human HepG2 cells after 72 hrs by trypan blue assay
Growth inhibition of human HepG2 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| HFL1 | GI50 |
>2 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human HFL1 cells after 72 hrs by trypan blue assay
Growth inhibition of human HFL1 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| HL-60 | GI50 |
0.125 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human HL60 cells after 72 hrs by trypan blue assay
Growth inhibition of human HL60 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| Jurkat | GI50 |
0.025 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human Jurkat cells after 72 hrs by trypan blue assay
Growth inhibition of human Jurkat cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| K562 | IC50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human K562 cells after 72 hrs by trypan blue assay
Growth inhibition of human K562 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| LNCaP | GI50 |
0.04 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human LNCAP cells after 72 hrs by trypan blue assay
Growth inhibition of human LNCAP cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| MCF7 | GI50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human MCF7 cells after 72 hrs by trypan blue assay
Growth inhibition of human MCF7 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| MDA-MB-436 | GI50 |
0.15 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human MDA-MB-436 cells after 72 hrs by trypan blue assay
Growth inhibition of human MDA-MB-436 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| MDA-MB-453 | GI50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human MDA-MB-453 cells after 72 hrs by trypan blue assay
Growth inhibition of human MDA-MB-453 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| MIA PaCa-2 | GI50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human MIAPaCa2 cells after 72 hrs by trypan blue assay
Growth inhibition of human MIAPaCa2 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| NCI-H1650 | GI50 |
0.2 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human NCI-H1650 cells after 72 hrs by trypan blue assay
Growth inhibition of human NCI-H1650 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| NCI-H1734 | GI50 |
0.15 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human NCI-H1734 cells after 72 hrs by trypan blue assay
Growth inhibition of human NCI-H1734 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| NCI-H1975 | GI50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human NCI-H1975 cells after 72 hrs by trypan blue assay
Growth inhibition of human NCI-H1975 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| NCI-H23 | GI50 |
0.2 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human H23 cells after 72 hrs by trypan blue assay
Growth inhibition of human H23 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| NCI-H292 | GI50 |
0.15 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human NCI-H292 cells after 72 hrs by trypan blue assay
Growth inhibition of human NCI-H292 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| NCI-H460 | GI50 |
0.1 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human H460 cells after 72 hrs by trypan blue assay
Growth inhibition of human H460 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| Panc1005 | GI50 |
0.4 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human Panc 10.05 cells after 72 hrs by trypan blue assay
Growth inhibition of human Panc 10.05 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| PC-3 | GI50 |
0.15 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human PC3 cells after 72 hrs by trypan blue assay
Growth inhibition of human PC3 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| PLC-PRF-5 | GI50 |
0.75 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human PLC/PRF/5 cells after 72 hrs by trypan blue assay
Growth inhibition of human PLC/PRF/5 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| Raji | GI50 |
0.05 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human Raji cells after 72 hrs by trypan blue assay
Growth inhibition of human Raji cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| RT-4 | GI50 |
0.5 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human RT4 cells after 72 hrs by trypan blue assay
Growth inhibition of human RT4 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| SK-HEP1 | GI50 |
0.1 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human SKHEP1 cells after 72 hrs by trypan blue assay
Growth inhibition of human SKHEP1 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| SK-MEL-28 | GI50 |
0.2 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human SK-MEL-28 cells after 72 hrs by trypan blue assay
Growth inhibition of human SK-MEL-28 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| SK-OV-3 | GI50 |
0.075 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human SKOV3 cells after 72 hrs by trypan blue assay
Growth inhibition of human SKOV3 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| SNU-398 | GI50 |
0.15 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human SNU398 cells after 72 hrs by trypan blue assay
Growth inhibition of human SNU398 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| SNU-449 | GI50 |
0.4 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human SNU449 cells after 72 hrs by trypan blue assay
Growth inhibition of human SNU449 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| SNU-475 | GI50 |
7.5 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human SNU475 cells after 72 hrs by trypan blue assay
Growth inhibition of human SNU475 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| SU.86.86 | GI50 |
0.4 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human SU8686 cells after 72 hrs by trypan blue assay
Growth inhibition of human SU8686 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| SW780 | GI50 |
0.15 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human SW780 cells after 72 hrs by trypan blue assay
Growth inhibition of human SW780 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| T-24 | GI50 |
0.15 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human T24 cells after 72 hrs by trypan blue assay
Growth inhibition of human T24 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| TCC-SUP | GI50 |
0.25 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human TCCSUP cells after 72 hrs by trypan blue assay
Growth inhibition of human TCCSUP cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| U2OS | GI50 |
0.035 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human U2OS cells after 72 hrs by trypan blue assay
Growth inhibition of human U2OS cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| U-87MG ATCC | GI50 |
0.02 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human U87 cells after 72 hrs by trypan blue assay
Growth inhibition of human U87 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| U-937 | GI50 |
0.125 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human U937 cells after 72 hrs by trypan blue assay
Growth inhibition of human U937 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| UMUC3 | GI50 |
0.3 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human UM-UC-3 cells after 72 hrs by trypan blue assay
Growth inhibition of human UM-UC-3 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| WI-38 | GI50 |
>5 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human WI38 cells after 72 hrs by trypan blue assay
Growth inhibition of human WI38 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
| Z-138 | GI50 |
0.2 μM
Compound: 7ao; ON1231320
|
Growth inhibition of human Z138 cells after 72 hrs by trypan blue assay
Growth inhibition of human Z138 cells after 72 hrs by trypan blue assay
|
[PMID: 26762835] |
ON1231320 (Compound 7ao) has no inhibitory activity against PLK1, PLK3 and PLK4 (all IC50>10 μM)[1].
ON1231320 (0-5 μM; 24 hours) activates programmed cell death in human tumor cells[1].
ON1231320 inhibits cell proliferation in 16 tumor cell lines (DU145, MCF-7, BT474, SK-OV-3, MIA-PaCa-2, SK-MEL-28, A549, U87, COLO-205, HELA, H1975, RAJI, U205, K562, GRANTA-519; IC50= 0.035-0.2 μM)[1].
ON1231320 does not appreciably inhibit tubulin polymerization[1]. ON1231320 does not affect normal human fibroblasts[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:U2OS cells
-
Concentration:0-5 µM
-
Incubation Time:24 hours
-
Result:Increased the activity of Caspases 3/7 in a dose-dependent manner.
Induced apoptosis.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:6-8 week old NCR nu/nu female mice with MDAMB-231 triple negative breast cancer cells[1]
-
Dosage:75 mg/kg
-
Administration:IP; alternate days (Q2D) for 20 days
-
Result:Resulted in significant inhibition of tumor growth (86.5%)
化学情報
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CAS 番号 1312471-39-8
-
性状 Solid
-
分子量 467.45
-
分子式 C22H15F2N5O3S
-
Color Light yellow to green yellow
-
SMILES
O=C1C(S(=O)(C2=CC=C(F)C=C2F)=O)=CC3=CN=C(NC4=CC5=C(NC=C5)C=C4)N=C3N1C
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
-
Most Recent
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Comput Struct Biotechnol J
A deep tabular data learning model predicting cisplatin sensitivity identifies BCL2L1 dependency in cancer. [Abstract]2023 Jan 16:21:956-964. PMID: 36733702 -
Transl Cancer Res
Integrative analysis of polo-like kinase family identifies a prognostic signature and validates PLK2 as a therapeutic target in glioma. [Abstract]2025 Oct 31;14(10):6348-6363. PMID: 41234853 -
溶剤 & 溶解度
DMSO : 50 mg/mL (106.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.35 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (276 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1393 mL | 10.6963 mL | 21.3927 mL | 53.4817 mL |
| 5 mM | 0.4279 mL | 2.1393 mL | 4.2785 mL | 10.6963 mL | |
| 10 mM | 0.2139 mL | 1.0696 mL | 2.1393 mL | 5.3482 mL | |
| 15 mM | 0.1426 mL | 0.7131 mL | 1.4262 mL | 3.5654 mL | |
| 20 mM | 0.1070 mL | 0.5348 mL | 1.0696 mL | 2.6741 mL | |
| 25 mM | 0.0856 mL | 0.4279 mL | 0.8557 mL | 2.1393 mL | |
| 30 mM | 0.0713 mL | 0.3565 mL | 0.7131 mL | 1.7827 mL | |
| 40 mM | 0.0535 mL | 0.2674 mL | 0.5348 mL | 1.3370 mL | |
| 50 mM | 0.0428 mL | 0.2139 mL | 0.4279 mL | 1.0696 mL | |
| 60 mM | 0.0357 mL | 0.1783 mL | 0.3565 mL | 0.8914 mL | |
| 80 mM | 0.0267 mL | 0.1337 mL | 0.2674 mL | 0.6685 mL | |
| 100 mM | 0.0214 mL | 0.1070 mL | 0.2139 mL | 0.5348 mL |