製品番号: HY-103504

(S)-SNAP5114

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

(S)-SNAP5114 is a non-covalent murine GABA transporter inhibitor with blood-brain barrier penetration ability, which exhibits significant subtype-selective inhibitory activity against mGAT4 (pIC₅₀=5.71, pK_i=4.56), much higher than its effects on mGAT1, mGAT2 and mGAT3. (S)-SNAP5114 elevates extracellular GABA concentrations by blocking the GABA reuptake mechanism, thereby enhancing thalamus-specific GABAergic signaling and exerting potential neuromodulatory effects. (S)-SNAP5114 is widely used in studies related to epilepsy, neuropathic pain, anxiety and depression, and various neurodegenerative diseases.

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純度: 98.09%
CAS 番号: 157604-55-2
分子式: C₃₀H₃₅NO₆
分子量:505.60

保管条件:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

(S)-SNAP5114 構造式

CAS 番号: 157604-55-2

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生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
化学情報
Publications (1)
溶剤 & 溶解度
- 体外
- 体内
純度とドキュメンテーション
参考文献
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target

IC50: 5 μM (hGAT-3), 21 μM (rGAT-2)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	1.12 μM Compound: 7, (S)-SNAP-5114	Inhibition of human GAT3 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis Inhibition of human GAT3 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis	[PMID: 23886812]
CHO	IC₅₀	11.9 μM Compound: 7, (S)-SNAP-5114	Inhibition of human GAT2 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis Inhibition of human GAT2 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis	[PMID: 23886812]
CHO	IC₅₀	170 μM Compound: 7, (S)-SNAP-5114	Inhibition of human GAT1 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis Inhibition of human GAT1 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis	[PMID: 23886812]
CHO	IC₅₀	71.7 μM Compound: 7, (S)-SNAP-5114	Inhibition of human BGT1 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis Inhibition of human BGT1 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis	[PMID: 23886812]
CHO	IC₅₀	>100 μM Compound: SNAP5114	Inhibition of human GAT1 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis Inhibition of human GAT1 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis	[PMID: 25147609]
CHO	IC₅₀	1.12 μM Compound: SNAP5114	Inhibition of human GAT3 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis Inhibition of human GAT3 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis	[PMID: 25147609]
CHO	IC₅₀	11.9 μM Compound: SNAP5114	Inhibition of human GAT2 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis Inhibition of human GAT2 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis	[PMID: 25147609]
CHO	IC₅₀	71.7 μM Compound: SNAP5114	Inhibition of human BGT1 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis Inhibition of human BGT1 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis	[PMID: 25147609]
HEK293	IC₅₀	22 μM Compound: 7, (S)-SNAP-5114	Inhibition of mouse GAT3-mediated [3H]GABA uptake expressed in human HEK cells Inhibition of mouse GAT3-mediated [3H]GABA uptake expressed in human HEK cells	[PMID: 21353350]
HEK293	IC₅₀	3 μM Compound: 7, (S)-SNAP-5114	Inhibition of mouse GAT4-mediated [3H]GABA uptake expressed in human HEK cells Inhibition of mouse GAT4-mediated [3H]GABA uptake expressed in human HEK cells	[PMID: 21353350]
HEK293	IC₅₀	35 μM Compound: 7, (S)-SNAP-5114	Inhibition of mouse GAT2-mediated [3H]GABA uptake expressed in human HEK cells Inhibition of mouse GAT2-mediated [3H]GABA uptake expressed in human HEK cells	[PMID: 21353350]
HEK293	IC₅₀	85 μM Compound: 7, (S)-SNAP-5114	Inhibition of mouse GAT1-mediated [3H]GABA uptake expressed in human HEK cells Inhibition of mouse GAT1-mediated [3H]GABA uptake expressed in human HEK cells	[PMID: 21353350]
HEK293	IC₅₀	>30 μM Compound: (S)-1b, SNAP 5114	Inhibition of mouse mGAT1 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis Inhibition of mouse mGAT1 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis	[PMID: 25882526]
HEK293	IC₅₀	20 μM Compound: (S)-1b, SNAP 5114	Inhibition of mouse mGAT3 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis Inhibition of mouse mGAT3 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis	[PMID: 25882526]
HEK293	IC₅₀	22 μM Compound: (S)-1b, SNAP 5114	Inhibition of mouse mGAT2 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis Inhibition of mouse mGAT2 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis	[PMID: 25882526]
HEK293	IC₅₀	6 μM Compound: (S)-1b, SNAP 5114	Inhibition of mouse mGAT4 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis Inhibition of mouse mGAT4 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis	[PMID: 25882526]
HEK293	IC₅₀	199 μM Compound: 23	Inhibition of mouse GAT1 mediated [3]GABA uptake expressed in HEK293 cells after 3 mins by scintillation counting Inhibition of mouse GAT1 mediated [3]GABA uptake expressed in HEK293 cells after 3 mins by scintillation counting	[PMID: 26706177]
HEK293	IC₅₀	21.9 μM Compound: 23	Inhibition of mouse GAT2 mediated [3]GABA uptake expressed in HEK293 cells after 3 mins by scintillation counting Inhibition of mouse GAT2 mediated [3]GABA uptake expressed in HEK293 cells after 3 mins by scintillation counting	[PMID: 26706177]
HEK293	IC₅₀	6.3 μM Compound: 23	Inhibition of mouse GAT3 mediated [3]GABA uptake expressed in HEK293 cells after 3 mins by scintillation counting Inhibition of mouse GAT3 mediated [3]GABA uptake expressed in HEK293 cells after 3 mins by scintillation counting	[PMID: 26706177]
HEK293	IC₅₀	>1000 μM Compound: 23	Inhibition of mouse BGT1 mediated [3]GABA uptake expressed in HEK293 cells after 3 mins by scintillation counting Inhibition of mouse BGT1 mediated [3]GABA uptake expressed in HEK293 cells after 3 mins by scintillation counting	[PMID: 26706177]

体外実験

(S)-SNAP-5114 (with 25 min pre-incubation) inhibits [³H]GABA uptake in HEK293 cells expressing mGAT1, mGAT3 and mGAT4, with pIC₅₀ values of 4.07, 5.29 and 5.71, respectively; it only exhibits weak inhibitory activity against mGAT2 at a concentration of 100 μM^[1].
(S)-SNAP5114 exhibits poor chemical stability in acidic media, with a half-life of approximately 4 h at pH 4.5^[3].
The calculated log D value of (S)-SNAP5114 at pH 7.4 is close to 2, suggesting its potential blood-brain barrier penetration property^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

化学情報

CAS 番号 157604-55-2
性状 Solid
分子量 505.60
分子式 C₃₀H₃₅NO₆
Color White to off-white
SMILES

O=C([C@@H]1CN(CCOC(C2=CC=C(OC)C=C2)(C3=CC=C(OC)C=C3)C4=CC=C(OC)C=C4)CCC1)O
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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iScience

Synaptic scaling of corticostriatal circuits underlies hyperactivity in GABA Transporter-1 deficient mice. [Abstract]

2023 Mar 3;26(4):106322. PMID: 36968092

溶剤 & 溶解度

体外:

DMSO : 200 mg/mL (395.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9778 mL

9.8892 mL

19.7785 mL

5 mM

0.3956 mL

1.9778 mL

3.9557 mL

10 mM

0.1978 mL

0.9889 mL

1.9778 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 5 mg/mL (9.89 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 5 mg/mL (9.89 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

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元素分析報告書 (2659 KB)
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OR (2659 KB)
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Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Böck MC, et al. N-Substituted Nipecotic Acids as (S)-SNAP-5114 Analogues with Modified Lipophilic Domains. ChemMedChem. 2020;15(9):756-771. [Content Brief]

[2]. Böck M C. Development of new mGAT4 inhibitors by variation of the amino acid subunit and lipophilic domain of (S)-SNAP-5114[D]. lmu, 2020.

[3]. Pabel J, et al. Development of an (S)-1-{2-[tris(4-methoxyphenyl)methoxy]ethyl}piperidine-3-carboxylic acid [(S)-SNAP-5114] carba analogue inhibitor for murine γ-aminobutyric acid transporter type 4. ChemMedChem. 2012;7(7):1245-1255. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9778 mL	9.8892 mL	19.7785 mL	49.4462 mL
	5 mM	0.3956 mL	1.9778 mL	3.9557 mL	9.8892 mL
	10 mM	0.1978 mL	0.9889 mL	1.9778 mL	4.9446 mL
	15 mM	0.1319 mL	0.6593 mL	1.3186 mL	3.2964 mL
	20 mM	0.0989 mL	0.4945 mL	0.9889 mL	2.4723 mL
	25 mM	0.0791 mL	0.3956 mL	0.7911 mL	1.9778 mL
	30 mM	0.0659 mL	0.3296 mL	0.6593 mL	1.6482 mL
	40 mM	0.0494 mL	0.2472 mL	0.4945 mL	1.2362 mL
	50 mM	0.0396 mL	0.1978 mL	0.3956 mL	0.9889 mL
	60 mM	0.0330 mL	0.1648 mL	0.3296 mL	0.8241 mL
	80 mM	0.0247 mL	0.1236 mL	0.2472 mL	0.6181 mL
	100 mM	0.0198 mL	0.0989 mL	0.1978 mL	0.4945 mL

Keywords

(S)-SNAP5114

1 Cited Publications

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(S)-SNAP5114

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

CNS-Penetrant Compound Library

Neurotransmitter Receptor Compound Library

Anti-Alzheimer's Disease Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

GABA Receptor Compound Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

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MedChemExpress（MCE）の使用を引用している文献 (S)-SNAP5114

生物活性

化学情報

Publications (1)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

(S)-SNAP5114

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

CNS-Penetrant Compound Library

Neurotransmitter Receptor Compound Library

Anti-Alzheimer's Disease Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

GABA Receptor Compound Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

MedChemExpress（MCE）の使用を引用している文献 (S)-SNAP5114

生物活性

化学情報

Publications (1)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

(S)-SNAP5114 関連分類

HY-103504 関連分類