Cat. No.: HY-N0773

Isovitexin

(Synonyms: Saponaretin; Homovitexin)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Isovitexin is a flavonoid isolated from passion flower, Cannabis and, and the palm, possesses anti-inflammatory and anti-oxidant activities; Isovitexin acts like a JNK1/2 inhibitor and inhibits the activation of NF-κB.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 38953-85-4
Formula: C₂₁H₂₀O₁₀
Molecular Weight:432.38

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Isovitexin Chemical Structure

CAS No.: 38953-85-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

JNK1

JNK2

NF-κB

All JNK Isoforms :

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	29.79 μM Compound: 39; Isov	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell viability measured after 24 hrs by CCK-8 method Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell viability measured after 24 hrs by CCK-8 method	[PMID: 38142509]
HCT-15	IC₅₀	>50 μM Compound: 7	Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
HepG2	IC₅₀	>50 μM Compound: 7	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
HL-60	IC₅₀	>50 μM Compound: 7	Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
HT-29	IC₅₀	37.24 μM Compound: 39; Isov	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell viability measured after 24 hrs by CCK-8 method Antiproliferative activity against human HT-29 cells assessed as inhibition of cell viability measured after 24 hrs by CCK-8 method	[PMID: 38142509]
Jurkat	IC₅₀	>50 μM Compound: 7	Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
THP-1	IC₅₀	>50 μM Compound: 7	Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
U-937	IC₅₀	>50 μM Compound: 7	Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]

In Vitro

Isovitexin protects against LPS-induced oxidative damage by suppressing intracellular ROS generation, and also attenuates the effect of H₂O₂ on cell viability. Isovitexin (0-100 μg/mL) with LPS (2 μg/mL) is not cytotoxic to RAW 264.7 cells, but 200 μg/mL Isovitexin shows significant cytotoxicity. Isovitexin (25, 50 μg/mL) inhibits LPS-induced increases in TNF-α, IL-6, iNOS, and COX-2 levels. Isovitexin (25, 50 μg/mL) also suppresses the IκBα phosphorylation and degradation in RAW 264.7 cells, and such an effect is consistent with that of JNK1/2 inhibitor^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Isovitexin (50 and 100 mg/kg, i.p.) causes less severe histopathological changes in the lung sections, and reduces inflammatory cell count in LPS-induced mice. Isovitexin (50 and 100 mg/kg, i.p.) protects against LPS-induced inflammation and oxidative stress in LPS-induced ALI mice by decreasing TNF-α and IL-6 production, ROS generation, and MPO and MDA content, increasing SOD and GSH levels and effectively inhibiting the protein expression of iNOS and COX-2^[1]. Isovitexin (25, 50, 100 mg/kg) dose-dependently reduces the survival rate of LPS/D-gal induced hepatic injury in mice. Isovitexin also inhibits NF-κB activation and up-regulates Nrf2 and HO-1 induced by LPS/D-gal in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 38953-85-4
Appearance Solid
Molecular Weight 432.38
Formula C₂₁H₂₀O₁₀
Color Light yellow to yellow
SMILES

OC1=C(C2=O)C(OC(C3=CC=C(O)C=C3)=C2)=CC(O)=C1[C@@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO
Synonyms

Saponaretin; Homovitexin
Structure Classification
- Flavonoids
- Flavones
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

All Cell Proliferation/Viability AssayWBRT-PCRELISAIF

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Journal Impact Factor

Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186
Food Chem

New insights into the influences of baking and storage on the nonvolatile compounds in oolong tea: A nontargeted and targeted metabolomics study. [Abstract]

2022 May 1:375:131872. PMID: 34953237
J Agric Food Chem

Identification and Functional Characterization of a Novel C-Glycosyltransferase from Euscaphis konishii Hayata and Its Role in C-Glycoside Biosynthesis. [Abstract]

2026 Feb 4;74(4):4059-4071. PMID: 41568575
Med Oncol

Identification of Isovitexin as a novel CYP17A1 inhibitor through virtual screening and evaluation of its anti-cancer effects in MCF-7 breast cancer cells. [Abstract]

2025 Mar 26;42(5):137. PMID: 40138050
J Inflamm Res

Isovitexin Depresses Osteoarthritis Progression via the Nrf2/NF-κB Pathway: An in vitro Study. [Abstract]

2021 Apr 13;14:1403-1414. PMID: 33883918

Isovitexin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Apr 13;14:1403-1414. [Abstract]

Assessment of IVX chondrocytic cells cytotoxicity, using differing concentrations of Isovitexin (IVX) (12, 25, 50, 100, 200, 400 μg/mL) for 24 hr and 48 hr.

Isovitexin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Apr 13;14:1403-1414. [Abstract]

Evaluation of ECM catabolic and anabolic protein expression in IL-1β- and Isovitexin (IVX) (25, 50 μg/mL)-treated chondrocytes, relative to control.

Isovitexin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Apr 13;14:1403-1414. [Abstract]

PCR analysis of inflammation-related transcripts after treated with Isovitexin (IVX) (25, 50 μg/mL).

Isovitexin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Apr 13;14:1403-1414. [Abstract]

Detection of inflammation-related protein expression, using ELISA after treated with Isovitexin (IVX) (25, 50 μg/mL).

Isovitexin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Apr 13;14:1403-1414. [Abstract]

Immunofluorescence demonstrated significant Nrf2 translocation to the nucleus, upon Isovitexin (IVX) (25, 50 μg/mL) treatment.
Tissue Cell

Isovitexin accelerates diabetic wound repair via coordinated angiogenesis and collagen remodeling: Mechanistic insights from cellular and streptozotocin-induced SD rat models. [Abstract]

2025 Aug 22:97:103100. PMID: 40882326

Solvent & Solubility

In Vitro:

DMSO : 23.33 mg/mL (53.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3128 mL

11.5639 mL

23.1278 mL

5 mM

0.4626 mL

2.3128 mL

4.6256 mL

10 mM

0.2313 mL

1.1564 mL

2.3128 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[1]

Cell viability is determined by an MTT assay. RAW 264.7 cells are plated in 96-well plates (1 × 10⁴ cells/well) and incubated with various concentrations of Isovitexin (final concentration: 0-200 μg/mL) and LPS (2 μg/mL) for 24 h. In addition, the cells are pretreated with IV (25 or 50 μg/mL) for 1 h, followed by the addition of H₂O₂ (300 μM). After 24 h, MTT (5 mg/mL) is added to the cells, which are then incubated for another 4 h^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[1]

Mice^[1]
To create an ALI model, mice are randomLy divided into six groups: control (saline), Isovitexin only (100 mg/kg, dissolved in 0.5% DMSO), LPS only (0.5 mg/kg, dissolved in saline), LPS (0.5 mg/kg) + Isovitexin (50 or 100 mg/kg), and LPS (0.5 mg/kg) + dexamethasone (Dex, 5 mg/kg dissolved in saline). Isovitexin or Dex (5 mg/kg) are administered Isovitexin. After exposure to Isovitexin or Dex for 1 h, the mice are anesthetized with diethyl ether, and LPS is administered intranasally (i.n.) to induce lung injury. After LPS administration for 12 h, the animals are euthanized. Accordingly, bronchoalveolar lavage fluid (BALF) and lung tissue samples are harvested to measure cytokine levels; ROS generation; SOD, GSH, MDA and MPO activity; and COX-2, iNOS, HO-1, and Nrf2 protein expression^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lv H, et al. Isovitexin Exerts Anti-Inflammatory and Anti-Oxidant Activities on Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting MAPK and NF-κB and Activating HO-1/Nrf2 Pathways. Int J Biol Sci. 2016 Jan 1;12(1):72-86. [Content Brief]

[2]. Hu JJ, et al. Isovitexin alleviates liver injury induced by lipopolysaccharide/d-galactosamine by activating Nrf2 and inhibiting NF-κB activation. Microb Pathog. 2018 Mar 29;119:86-92. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3128 mL	11.5639 mL	23.1278 mL	57.8195 mL
	5 mM	0.4626 mL	2.3128 mL	4.6256 mL	11.5639 mL
	10 mM	0.2313 mL	1.1564 mL	2.3128 mL	5.7820 mL
	15 mM	0.1542 mL	0.7709 mL	1.5419 mL	3.8546 mL
	20 mM	0.1156 mL	0.5782 mL	1.1564 mL	2.8910 mL
	25 mM	0.0925 mL	0.4626 mL	0.9251 mL	2.3128 mL
	30 mM	0.0771 mL	0.3855 mL	0.7709 mL	1.9273 mL
	40 mM	0.0578 mL	0.2891 mL	0.5782 mL	1.4455 mL
	50 mM	0.0463 mL	0.2313 mL	0.4626 mL	1.1564 mL

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Isovitexin

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

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Publications Citing Use of MedChemExpress (MCE) Isovitexin

All JNK Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Isovitexin Related Classifications

HY-N0773 Related Classifications