4-Thiothymidine
Based on 1 Customer Validation
4-Thiothymidine is a thymidine analog with UVA photosensitizing properties. 4-Thiothymidine incorporates into the DNA of dividing cells via the thymidine kinase-mediated salvage pathway, replacing thymine and sensitizing cells to UVA irradiation, thereby inducing apoptosis. 4-Thiothymidine induces DNA damage upon UVA activation and inhibits DNA replication. 4-Thiothymidine is used in research related to non-melanoma skin malignancies such as squamous cell carcinoma.
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- Purity : 99.69%
- CAS No.: 7236-57-9
- 화학식: C10H14N2O4S
- 분자량:258.29
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
4-Thiothymidine treatment (combined with UVA irradiation) induces pan-nuclear γ-H2AX staining in HaCaT cells, indicating replication inhibition rather than discrete focal double-strand breaks[1].
4‑Thiothymidine exhibits extremely potent cytotoxicity toward HR‑deficient xrcc2 hamster cells and XPF‑deficient UV41 cells under 0.1 kJ/m2 UVA conditions. S5‑(6‑4) T:T photoproducts are removed via nucleotide excision repair in HaCaT cells; these photoproducts are completely irreparable in XPA‑deficient XP12RO cells and exhibit significantly reduced repair capacity in CCRF‑CEM leukemia cells[1].
4-Thiothymidine efficiently generates singlet oxygen in O2-saturated acetonitrile solution with a quantum yield of 0.42[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HaCaT
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Concentration:100 μM
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Incubation Time:48 h
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Result:Induced pan-nuclear γ-H2AX staining in most cells after 4 h of irradiation, consistent with inhibited replication.
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Cell Line:SCC-15 human squamous cell carcinoma cells
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Concentration:100 μM
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Incubation Time:24 h
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Result:Induced almost complete apoptosis in SCC-15 cells when combined with 10 kJ/m2 UV-A irradiation.
Chemical Information
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CAS No. 7236-57-9
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Appearance Solid
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분자량 258.29
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화학식 C10H14N2O4S
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Color Light yellow to light brown
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SMILES
OC[C@@H]1[C@@H](O)C[C@H](N2C(NC(C(C)=C2)=S)=O)O1
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
In Vitro:
DMSO : 116.67 mg/mL (451.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (288 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8716 mL | 19.3581 mL | 38.7162 mL | 96.7904 mL |
| 5 mM | 0.7743 mL | 3.8716 mL | 7.7432 mL | 19.3581 mL | |
| 10 mM | 0.3872 mL | 1.9358 mL | 3.8716 mL | 9.6790 mL | |
| 15 mM | 0.2581 mL | 1.2905 mL | 2.5811 mL | 6.4527 mL | |
| 20 mM | 0.1936 mL | 0.9679 mL | 1.9358 mL | 4.8395 mL | |
| 25 mM | 0.1549 mL | 0.7743 mL | 1.5486 mL | 3.8716 mL | |
| 30 mM | 0.1291 mL | 0.6453 mL | 1.2905 mL | 3.2263 mL | |
| 40 mM | 0.0968 mL | 0.4840 mL | 0.9679 mL | 2.4198 mL | |
| 50 mM | 0.0774 mL | 0.3872 mL | 0.7743 mL | 1.9358 mL | |
| 60 mM | 0.0645 mL | 0.3226 mL | 0.6453 mL | 1.6132 mL | |
| 80 mM | 0.0484 mL | 0.2420 mL | 0.4840 mL | 1.2099 mL | |
| 100 mM | 0.0387 mL | 0.1936 mL | 0.3872 mL | 0.9679 mL |
4-Thiothymidine Related Classifications
HY-W587829 Related Classifications
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