FIPI
Based on 5 publication(s) in Google Scholar
FIPI is a phospholipase D (PLD) inhibitor with an IC50 for PLD1 and PLD2 of about 25 nM. FIPI regulates cytoskeletal recombination, cell diffusion and chemotaxis. FIPI can be used in cancer research. In addition, FIPI can enhance the secretion and aggregation of platelet dense particles, inhibit thrombosis, reduce ischemic stroke infarct volume and improve nerve function.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 98.00%
- CAS No.: 939055-18-2
- 화학식: C23H24FN5O2
- 분자량:421.47
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Publications Citing Use of MedChemExpress (MCE) FIPI
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Migration/Invasion Assay
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Cell Migration/Invasion Assay
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Cell Migration/Invasion Assay
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IF
All Phospholipase Isoforms
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Biological Activity
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PLD1 |
PLD2 |
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Cell Line
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Type | Value | Description | References |
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| Calu-1 | IC50 |
1 nM
Compound: 14
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Inhibition of PLD1 in human Calu-1 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
Inhibition of PLD1 in human Calu-1 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
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[PMID: 19136975] |
| HEK293 | IC50 |
300 nM
Compound: 14
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Inhibition of GFP-tagged human PLD2 expressed in human HEK293 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
Inhibition of GFP-tagged human PLD2 expressed in human HEK293 cells assessed as decrease in phosphatidylbutanol-[d9] production after 30 mins by mass spectrometric analysis
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[PMID: 19136975] |
FIPI (750 nM; 30 min) effectively blocks the production of phosphatidic acid by PLD stimulated by PMA (HY-18739) in HEK293 cells[1].
FIPI (750 nM; 1-4 h) promotes the diffusion of CHO cells and inhibits the chemotaxis of neutrophils[1].
FIPI (1-100 nM; 1-45 min) inhibits cell migration and calcium release in EGF-induced MDA-MB-468-NEO (EGFR positive) and MDA-MB-468-HER2 (EGFR and HER2 positive)[2].
FIPI (10 μM; 2.5 min) enhances the secretion and aggregation of dense particles in platelets, and increases the phosphorylation of protein kinase C substrate Pleckstrin[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ferric chloride (HY-Y0266) or transient middle cerebral artery occlusion treated mice[4]
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Dosage:3 mg/kg
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Administration:Intraperitoneal injection (i.p.); 2 times, 12 h apart
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Result:Delayed the thrombus formation and only 3 of 9 vessels occluded during the 30-minute observation period in arterial thrombosis models.
Reduced the infarct volumes, improved motor and overall nerve function and did not impair normal hemostasis in ischemic stroke models.
Chemical Information
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CAS No. 939055-18-2
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Appearance Solid
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분자량 421.47
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화학식 C23H24FN5O2
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Color Light yellow to khaki
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SMILES
O=C(NCCN1CCC(CC1)N2C3=CC=CC=C3NC2=O)C4=CC5=C(C=CC(F)=C5)N4
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Synonyms
5-Fluoro-2-indolyl deschlorohalopemide
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (5)
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Journal Impact Factor
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Most Recent
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Anal Chem
Phospholipid-Tailored Titanium Carbide Nanosheets as a Novel Fluorescent Nanoprobe for Activity Assay and Imaging of Phospholipase D. [Abstract]2018 Jun 5;90(11):6742-6748. PMID: 29737155
FIPI purchased from MedChemExpress. Usage Cited in: Anal Chem. 2018 Jun 5;90(11):6742-6748. [Abstract]
Typical CSLM images of MDA-MB-231 cells incubated with 50 μg/mL RhB-PL-Ti3C2 for 4 h at 37°C in the absence (I) and presence of 1.0 mg/mL FIPI (II), and CSLM images of MCF-7 cells (III) incubated with 50 μg/mL RhB-PL-Ti3C2 for 4 h at 37°C.
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Biochem Pharmacol
Phospholipase D2 regulation by transcription factor FOXA1 promotes the drug resistance of vemurafenib in melanoma cells. [Abstract]2025 Dec;242(Pt 4):117434. PMID: 41110487 -
J Cell Sci
The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response. [Abstract]2018 Mar 16;131(6). pii: jcs207217. PMID: 29420297
FIPI purchased from MedChemExpress. Usage Cited in: J Cell Sci. 2018 Mar 16;131(6). pii: jcs207217. [Abstract]
ER:Ras IMR90 cells are treated with solvent or FIPI (250 nM, 2h).Western blotting are performed.
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Nanoscale Res Lett
Preparation and Evaluation of Liposomes Co-Loaded with Doxorubicin, Phospholipase D Inhibitor 5-Fluoro-2-Indolyl Deschlorohalopemide (FIPI) and D-Alpha Tocopheryl Acid Succinate (α-TOS) for Anti-Metastasis. [Abstract]2019 Apr 18;14(1):138. PMID: 31001703
FIPI purchased from MedChemExpress. Usage Cited in: Nanoscale Res Lett. 2019 Apr 18;14(1):138. [Abstract]
Cell viability of MDA-MB-231 cells incubated with Blank-lip, FIPI-lip, DF-lip, DT-lip, and DFT-lip for 7 h or 24 h at a concentration of 2 μM FIPI, respectively.
FIPI purchased from MedChemExpress. Usage Cited in: Nanoscale Res Lett. 2019 Apr 18;14(1):138. [Abstract]
Inhibition effects on the cell motility in vitro. Images of MDA-MB-231 cells wound edge after incubation with control, Blank-lip, FIPI-lip, DF-lip, DT-lip, and DFT-lip for 48 h, respectively (magnification, × 100).
FIPI purchased from MedChemExpress. Usage Cited in: Nanoscale Res Lett. 2019 Apr 18;14(1):138. [Abstract]
Inhibition effects on cell migration. Images of MDA-MB-231 cell migration after incubation with control, Blank-lip, FIPI-lip, DF-lip, DT-lip, and DFT-lip for 7 h at a concentration of 2 μM FIPI.
FIPI purchased from MedChemExpress. Usage Cited in: Nanoscale Res Lett. 2019 Apr 18;14(1):138. [Abstract]
Inhibition effects on the cell invasion. Images of MDA-MB-231 cell invasion after incubation with control, Blank-lip, FIPI-lip, DF-lip, DT-lip, and DFT-lip for 24 h a concentration of 2 μM FIPI (magnification, × 100).
용액&용해도
DMSO : 3.33 mg/mL (7.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Su W, et al. 5-Fluoro-2-indolyl des-chlorohalopemide (FIPI), a phospholipase D pharmacological inhibitor that alters cell spreading and inhibits chemotaxis. Mol Pharmacol. 2009 Mar;75(3):437-46. [Content Brief]
[2]. Stricker HM, et al. The phospholipase D inhibitor FIPI potently blocks EGF-induced calcium signaling in human breast cancer cells. Cell Commun Signal. 2021 Apr 8;19(1):43. [Content Brief]
[3]. Elvers M, et al. A novel role for phospholipase D as an endogenous negative regulator of platelet sensitivity. Cell Signal. 2012 Sep;24(9):1743-52. [Content Brief]
[4]. Stegner D, et al. Pharmacological inhibition of phospholipase D protects mice from occlusive thrombus formation and ischemic stroke--brief report. Arterioscler Thromb Vasc Biol. 2013 Sep;33(9):2212-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3726 mL | 11.8632 mL | 23.7265 mL | 59.3162 mL |
| 5 mM | 0.4745 mL | 2.3726 mL | 4.7453 mL | 11.8632 mL |
FIPI Related Classifications
HY-12807 Related Classifications
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