Anserinone B
Anserinone B is an antifungal and antibacterial benzoquinone. Anserinone B causes radial growth reductions of 50% and 37% against S.fimicola and A. furfuraceus, respectively. Anserinones B also displays moderate cytotoxicity in the NCI’s 60 human tumor cell line panel (GI50=4.4 µg/mL).
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- CAS No.: 190895-96-6
- 화학식: C11H14O4
- 분자량:210.23
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocol
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
제품 설명
IC50 & Target
[1]|
Microbial Metabolite |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| C6 | IC50 |
<10 μM
Compound: 8
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Cytotoxicity against rat C6 cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
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[PMID: 34787427] |
| HGC-27 | IC50 |
<10 μM
Compound: 8
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Cytotoxicity against human HGC-27 cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
Cytotoxicity against human HGC-27 cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
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[PMID: 34787427] |
| MDA-MB-435 | IC50 |
3.6 μg/mL
Compound: 2
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Cytotoxicity against human MDA-MB-435 after 48 hrs by SRB assay
Cytotoxicity against human MDA-MB-435 after 48 hrs by SRB assay
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[PMID: 15043411] |
| SH-SY5Y | IC50 |
<10 μM
Compound: 8
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Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell viability measured for 48 hrs by CCK colorimetric assay
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[PMID: 34787427] |
Chemical Information
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CAS No. 190895-96-6
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분자량 210.23
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화학식 C11H14O4
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SMILES
O=C(C=C1OC)C(C)=C(C[C@H](O)C)C1=O
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
순도&문서
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anserinone B Related Classifications
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