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CFDA-SE

(Synonyms: CFSE; 5(6)-Carboxyfluorescein diacetate succinimidyl ester; 5(6)-CFDA N-succinmidyl ester)
119 Cited Publications
고객리뷰

Based on 119 publication(s) in Google Scholar

CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells.

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  • Purity: 97.0%
  • CAS No.: 150347-59-4
  • 화학식: C29H19NO11
  • 분자량:557.46
  • 보관:

    -20°C, sealed storage, away from moisture and light

    * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

  • CFDA-SE Chemical Structure
    CFDA-SE Chemical Structure
  • Experimental Validation Results for CFDA-SE
  • Experimental Validation Results for CFDA-SE
  • Experimental Validation Results for CFDA-SE
  • Experimental Validation Results for CFDA-SE
  • Experimental Validation Results for CFDA-SE
CAS No.: 150347-59-4
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Cell Proliferation/Viability AssayFlow CytometryIn Vivo Imaging
  • Flow Cytometry
  • Cell Proliferation/Viability Assay
  • Cell Proliferation/Viability Assay
  • Cell Proliferation/Viability Assay
  • In Vivo Imaging
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  • CFDA-SE Chemical Structure
    CFDA-SE Chemical Structure
    1/16
  • 1/16

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Aug 10:541:215746. [Abstract]

    Customer Validation
     PBMCs are labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE) (2.5 μM). The proliferation of T cells is assessed by CFSE intensity using flow cytometry.

  • 1/16

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2201899119. [Abstract]

    Customer Validation
     Representative images of CFDA-SE (CFSE)-based proliferation assay of CD4+ T cells cocultured with AT2 cells from healthy, LLC-bearing GPX3fl/fl, or GPX3cKO mice respectively.

  • 1/16

    CFDA-SE purchased from MedChemExpress. Usage Cited in: OncoImmunology. 2022 Feb 1;11(1):2032918. [Abstract]

    Customer Validation
     Flow cytometric analysis of the proliferation rates of bone marrow-derived CD11b+F4/80+ cells as the function of time being cultured in the presence and absence of TNFSF15. Bone marrow cells are incubated with 5 μM CFSE (CFDA-SE) for 10 min at 37°C.

  • 1/16

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Feb 20;11(9):4251-4261. [Abstract]

    Customer Validation
     After mouse BMDMs were pretreated with PBS (control; CON), heparin (HEP, 3 U/mL), warfarin (WAR, 100 ng/mL), dabigatran (DAB, 100 ng/mL), TMI-1 (10 µM), and baicalein (Bai, 10 µM) for 4 h, they were co-cultured with CFDA-SE (CFSE, 1 μM; 30 min)-labeled apoptotic MC38 cells at the ratio of 1:10 for 30 min, efferocytosis was performed with flow cytometry.

  • 1/16

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Mol Nutr Food Res. 2022 Jan;66(2):e2100619. [Abstract]

    Customer Validation
     CFDA-SE (10 μM) was added to bacterial suspension and then incubated at 37°C in the dark for 20 min. The four strains were labeled with fluorescent probe CFDA-SE and were gavaged to baby rats to evaluate their colonization ability. Compared to the control group, the CFDA-SE fluorescence was present in rats gavaged with the four strains.

  • CFDA-SE